NCKAP1 (NM_205842) Human Untagged Clone

CAT#: SC308232

NCKAP1 (untagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCKAP1
• Synonyms: HEM2; NAP1; NAP125; p125Nap1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_205842, the custom clone sequence may differ by one or more nucleotides
  ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
  AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGCAAGGACAAGTT
  TGGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAA
  TCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAA
  CAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTAC
  TTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATT
  GACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA
  GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAG
  GCAATCATTGGATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAA
  TACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAA
  GAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATAT
  CCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGT
  GCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCT
  TTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT
  ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCT
  CTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATA
  CGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACAT
  GCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCT
  ACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCA
  TTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAG
  AAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA
  CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTT
  TCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAA
  GATGAATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAA
  GTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAG
  GCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAG
  ATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTG
  GAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA
  CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACT
  CATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGC
  AGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATC
  ACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCC
  AAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAA
  CCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAAC
  CTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC
  ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATA
  CGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCA
  AAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAA
  AACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAA
  CATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAA
  ACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTT
  GTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA
  GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGC
  CTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTT
  GATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTT
  AAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTA
  TCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTT
  CTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTT
  GCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA
  TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAA
  ATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTG
  ATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAA
  GCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGT
  CTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGAT
  AAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAA
  TCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT
  GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_205842
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205842.1, NP_995314.1
• RefSeq Size: 4637 bp
• RefSeq ORF: 3405 bp
• Locus ID: 10787
• UniProt ID: Q9Y2A7
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.