GPR161 (NM_153832) Human Tagged ORF Clone

CAT#: RC215665

GPR161 (Myc-DDK-tagged)-Human G protein-coupled receptor 161 (GPR161), transcript variant 2

Product Images

Circular map for RC215665

• 

  Western blot validation of overexpression lysate (Cat# LY403522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR161
• Synonyms: RE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215665 representing NM_153832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCAACTCCTCCCTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTGAGGAGGAGGGTGGCGAAG
  GGGGCGTCATCATCACCCAGTTCATCGCCATCATTGTCATCACCATTTTTGTCTGCCTGGGAAACCTGGT
  CATCGTGGTCACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGTTCGTCTTCAGCCTGACT
  CTGTCCAACTTCCTGCTGTCCGTGTTGGTGCTGCCTTTTGTGGTGACGAGCTCCATCCGCAGGGAATGGA
  TCTTTGGTGTAGTGTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAAC
  CCTCGGGGTCATTGCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACA
  GGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCCACCCCTGT
  TTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGGGAGCCTGG
  CTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCTATGGCTTC
  ATCTTCCGCGTGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATG
  CTCAGAGGACCGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCA
  GGGTGTGGTCTACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATG
  GTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGTCTCCCCGA
  GCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTATGGACTCTG
  GAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAACCATTTGTG
  CAACGACAGAGGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACC
  TCACTGCGCTCATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTG
  CTCCCAGGACTCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCAC
  TGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCTG
  CCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTGGC
  CAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTTACA
  GCACGGACTGTCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGC
  AGTTGCAGAGCATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215665 representing NM_153832
  Red=Cloning site Green=Tags(s)
  
  MSLNSSLSCRKELSNLTEEEGGEGGVIITQFIAIIVITIFVCLGNLVIVVTLYKKSYLLTLSNKFVFSLT
  LSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGVIAIDRYYAVLYPMVYPMKIT
  GNRAVMALVYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALFPFLVMLVCYGF
  IFRVARVKARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRNAFQGVVYSANQCKALITILVVLGAFM
  VTWGPYMVVIASEALWGKSSVSPSLETWATWLSFASAVCHPLIYGLWNKTVRKELLGMCFGDRYYREPFV
  QRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQDSGTDMMLLEDYTSDDNPPSH
  CTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAIEAEAKINLFGEEALPGVLVT
  ARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153832
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153832.3
• RefSeq Size: 8222 bp
• RefSeq ORF: 1590 bp
• Locus ID: 23432
• UniProt ID: Q8N6U8
• Cytogenetics: 1q24.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 59 kDa
• Gene Summary: The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017]