GPR161 (NM_153832) Human Untagged Clone

CAT#: SC108456

GPR161 (untagged)-Human G protein-coupled receptor 161 (GPR161), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR161
• Synonyms: RE2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153832, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTCAACTCCTCCCTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTGAGGAGGAGGGTGGCGAAG
  GGGGCGTCATCATCACCCAGTTCATCGCCATCATTGTCATCACCATTTTTGTCTGCCTGGGAAACCTGGT
  CATCGTGGTCACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGTTCGTCTTCAGCCTGACT
  CTGTCCAACTTCCTGCTGTCCGTGTTGGTGCTGCCTTTTGTGGTGACGAGCTCCATCCGCAGGGAATGGA
  TCTTTGGTGTAGTGTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAAC
  CCTCGGGGTCATTGCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACA
  GGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCCACCCCTGT
  TTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGGGAGCCTGG
  CTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCTATGGCTTC
  ATCTTCCGCGTGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATG
  CTCAGAGGACCGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCA
  GGGTGTGGTCTACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATG
  GTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGTCTCCCCGA
  GCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTATGGACTCTG
  GAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAACCATTTGTG
  CAACGACAGAGGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACC
  TCACTGCGCTCATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTG
  CTCCCAGGACTCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCAC
  TGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCTG
  CCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTGGC
  CAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTTACA
  GCACGGACTGTCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGC
  AGTTGCAGAGCATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAGATGA
  
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153832
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153832.2, NP_722561.1
• RefSeq Size: 8222 bp
• RefSeq ORF: 1590 bp
• Locus ID: 23432
• UniProt ID: Q8N6U8
• Cytogenetics: 1q24.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (2) is shorter compared to isoform 1. Variants 2, 3, 8, 9, and 10 all encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.