KIRREL2 (NM_032123) Human Tagged ORF Clone

CAT#: RC215296

KIRREL2 (Myc-DDK-tagged)-Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1

Product Images

Circular map for RC215296

• 

  Western blot validation of overexpression lysate (Cat# LY410052) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215296 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIRREL2
• Synonyms: FILTRIN; NEPH3; NLG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215296 representing NM_032123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGGATGCGGGTCCCCGCCCTCCTCGTCCTCCTCTTCTGCTTCAGAGGGAGAGCAGGCCCGTCGC
  CCCATTTCCTGCAACAGCCAGAGGACCTGGTGGTGCTGCTGGGGGAGGAAGCCCGGCTGCCGTGTGCTCT
  GGGCGCCTACTGGGGGCTAGTTCAGTGGACTAAGAGTGGGCTGGCCCTAGGGGGCCAAAGGGACCTACCA
  GGGTGGTCCCGGTACTGGATATCAGGGAATGCAGCCAATGGCCAGCATGACCTCCACATTAGGCCCGTGG
  AGCTAGAGGATGAAGCATCATATGAATGTCAGGCTACACAAGCAGGCCTCCGCTCCAGACCAGCCCAACT
  GCACGTGCTGGTCCCCCCAGAAGCCCCCCAGGTGCTGGGCGGCCCCTCTGTGTCTCTGGTTGCTGGAGTT
  CCTGCGAACCTGACATGTCGGAGCCGTGGGGATGCCCGCCCTACCCCTGAATTGCTGTGGTTCCGAGATG
  GGGTCCTGTTGGATGGAGCCACCTTCCATCAGACCCTGCTGAAGGAAGGGACCCCTGGGTCAGTGGAGAG
  CACCTTAACCCTGACCCCTTTCAGCCATGATGATGGAGCCACCTTTGTCTGCCGGGCCCGGAGCCAGGCC
  CTGCCCACAGGAAGAGACACAGCTATCACACTGAGCCTGCAGTACCCCCCAGAGGTGACTCTGTCTGCTT
  CGCCACACACTGTGCAGGAGGGAGAGAAGGTCATTTTCCTGTGCCAGGCCACAGCCCAGCCTCCTGTCAC
  AGGCTACAGGTGGGCAAAAGGGGGCTCTCCGGTGCTCGGGGCCCGCGGGCCAAGGTTAGAGGTCGTGGCA
  GACGCCTCGTTCCTGACTGAGCCCGTGTCCTGCGAGGTCAGCAACGCCGTGGGTAGCGCCAACCGCAGTA
  CTGCGCTGGATGTGCTGTTTGGGCCGATTCTGCAGGCAAAGCCGGAGCCCGTGTCCGTGGACGTGGGGGA
  AGACGCTTCCTTCAGCTGCGCCTGGCGCGGGAACCCGCTTCCACGGGTAACCTGGACCCGCCGCGGTGGC
  GCGCAGGTGCTGGGCTCTGGAGCCACACTGCGTCTTCCGTCGGTGGGGCCCGAGGACGCAGGCGACTATG
  TGTGCAGAGCTGAGGCTGGGCTATCGGGCCTGCGGGGCGGCGCCGCGGAGGCTCGGCTGACTGTGAACGC
  TCCCCCAGTAGTGACCGCCCTGCACTCTGCGCCTGCCTTCCTGAGGGGCCCTGCTCGCCTCCAGTGTCTG
  GTTTTCGCCTCTCCCGCCCCAGATGCCGTGGTCTGGTCTTGGGATGAGGGCTTCCTGGAGGCGGGGTCGC
  AGGGCCGGTTCCTGGTGGAGACATTCCCTGCCCCAGAGAGCCGCGGGGGACTGGGTCCGGGCCTGATCTC
  TGTGCTACACATTTCGGGGACCCAGGAGTCTGACTTTAGCAGGAGCTTTAACTGCAGTGCCCGGAACCGG
  CTGGGCGAGGGAGGTGCCCAGGCCAGCCTGGGCCGTAGAGACTTGCTGCCCACTGTGCGGATAGTGGCCG
  GAGTGGCCGCTGCCACCACAACTCTCCTTATGGTCATCACTGGGGTGGCCCTCTGCTGCTGGCGCCACAG
  CAAGGCCTCAGCCTCTTTCTCCGAGCAAAAGAACCTGATGCGAATCCCTGGCAGCAGCGACGGCTCCAGT
  TCACGAGGTCCTGAAGAAGAGGAGACAGGCAGCCGCGAGGACCGGGGCCCCATTGTGCACACTGACCACA
  GTGATCTGGTTCTGGAGGAGGAAGGGACTCTGGAGACCAAGGACCCAACCAACGGTTACTACAAGGTCCG
  AGGAGTCAGTCCACCCGCGTCTCCAGACTCACGTGTGACATCTTTCCAATGGAAGAGTCCTGGGATCTCC
  AACTTGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215296 representing NM_032123
  Red=Cloning site Green=Tags(s)
  
  MLRMRVPALLVLLFCFRGRAGPSPHFLQQPEDLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLP
  GWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAGLRSRPAQLHVLVPPEAPQVLGGPSVSLVAGV
  PANLTCRSRGDARPTPELLWFRDGVLLDGATFHQTLLKEGTPGSVESTLTLTPFSHDDGATFVCRARSQA
  LPTGRDTAITLSLQYPPEVTLSASPHTVQEGEKVIFLCQATAQPPVTGYRWAKGGSPVLGARGPRLEVVA
  DASFLTEPVSCEVSNAVGSANRSTALDVLFGPILQAKPEPVSVDVGEDASFSCAWRGNPLPRVTWTRRGG
  AQVLGSGATLRLPSVGPEDAGDYVCRAEAGLSGLRGGAAEARLTVNAPPVVTALHSAPAFLRGPARLQCL
  VFASPAPDAVVWSWDEGFLEAGSQGRFLVETFPAPESRGGLGPGLISVLHISGTQESDFSRSFNCSARNR
  LGEGGAQASLGRRDLLPTVRIVAGVAAATTTLLMVITGVALCCWRHSKASASFSEQKNLMRIPGSSDGSS
  SRGPEEEETGSREDRGPIVHTDHSDLVLEEEGTLETKDPTNGYYKVRGVSPPASPDSRVTSFQWKSPGIS
  NLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032123
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032123.7
• RefSeq Size: 2153 bp
• RefSeq ORF: 1902 bp
• Locus ID: 84063
• UniProt ID: Q6UWL6
• Cytogenetics: 19q13.12
• Protein Families: Transmembrane
• MW: 66.9 kDa
• Gene Summary: This gene encodes a type I transmembrane protein and member of the immunoglobulin superfamily of cell adhesion molecules. The encoded protein localizes to adherens junctions in pancreatic beta cells and regulates insulin secretion. Autoantibodies against the encoded protein have been detected in serum from patients with type 1 diabetes. This gene may also play a role in glomerular development and decreased expression of this gene has been observed in human glomerular diseases. This gene and the related opposite-strand gene nephrin (GeneID: 527362) are regulated by a bidirectional promoter. [provided by RefSeq, Jul 2016]