SLC9A1 (NM_003047) Human Tagged ORF Clone

CAT#: RC214890

SLC9A1 (Myc-DDK-tagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 1 (SLC9A1)

Product Images

Circular map for RC214890

• 

  Western blot validation of overexpression lysate (Cat# LY401064) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214890 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC9A1
• Synonyms: APNH; LIKNS; NHE-1; NHE1; PPP1R143
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214890 representing NM_003047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTGCGGTCTGGCATCTGTGGCCTCTCTCCACATCGGATCTTCCCTTCCTTACTCGTGGTGGTCG
  CTTTGGTGGGGCTGCTGCCTGTTCTCAGGAGCCATGGCCTCCAGCTCAGCCCAACTGCCAGCACCATTCG
  AAGCTCAGAGCCACCACGAGAACGCTCGATTGGGGATGTCACCACCGCTCCACCGGAGGTCACCCCAGAG
  AGCCGCCCTGTTAATCATTCCGTCACTGATCATGGCATGAAGCCGCGCAAGGCCTTTCCAGTCCTGGGCA
  TCGACTACACACACGTGCGCACCCCCTTCGAGATCTCCCTCTGGATCCTTCTGGCCTGCCTCATGAAGAT
  AGGTTTCCATGTGATCCCCACTATCTCAAGCATCGTCCCGGAGAGCTGCCTGCTGATCGTGGTGGGGCTG
  CTGGTGGGGGGCCTGATCAAGGGTGTAGGCGAGACACCCCCCTTCCTGCAGTCCGACGTCTTCTTCCTCT
  TCCTGCTGCCGCCCATCATCCTGGATGCGGGCTACTTCCTGCCACTGCGGCAGTTCACAGAAAACCTGGG
  CACCATCCTGATCTTTGCCGTGGTGGGCACGCTGTGGAACGCCTTCTTCCTGGGCGGCCTCATGTACGCC
  GTGTGCCTGGTGGGCGGTGAGCAGATCAACAACATCGGCCTCCTGGACAACCTGCTCTTCGGCAGCATCA
  TCTCGGCCGTGGACCCCGTGGCGGTTCTGGCTGTCTTTGAGGAAATTCACATCAATGAGCTGCTGCACAT
  CCTTGTTTTTGGGGAGTCCTTGCTCAATGACGCCGTCACTGTGGTCCTGTATCACCTCTTTGAGGAGTTT
  GCCAACTACGAACACGTGGGCATCGTGGACATCTTCCTCGGCTTCCTGAGCTTCTTCGTGGTGGCCCTGG
  GCGGGGTGCTTGTGGGCGTGGTCTACGGGGTCATCGCAGCCTTCACCTCCCGATTTACCTCCCACATCCG
  GGTCATCGAGCCGCTCTTCGTCTTCCTCTACAGCTACATGGCCTACTTGTCAGCCGAGCTCTTCCACCTG
  TCAGGCATCATGGCGCTCATAGCCTCAGGAGTGGTGATGCGCCCCTATGTGGAGGCCAACATCTCCCACA
  AGTCCCACACCACCATCAAATACTTCCTGAAGATGTGGAGCAGCGTCAGCGAGACCCTCATCTTCATCTT
  CCTCGGCGTCTCCACGGTGGCCGGCTCCCACCACTGGAACTGGACCTTCGTCATCAGCACCCTGCTCTTC
  TGCCTCATCGCCCGCGTGCTGGGGGTGCTGGGCCTGACCTGGTTCATCAACAAGTTCCGTATCGTGAAGC
  TGACCCCCAAGGACCAGTTCATCATCGCCTATGGGGGCCTGCGAGGGGCCATCGCCTTCTCTCTGGGCTA
  CCTCCTGGACAAGAAGCACTTCCCCATGTGTGACCTGTTCCTCACTGCCATCATCACTGTCATCTTCTTC
  ACCGTCTTTGTGCAGGGCATGACCATTCGGCCCCTGGTAGACCTGTTGGCTGTGAAGAAAAAGCAAGAGA
  CGAAGCGCTCCATCAACGAAGAGATCCACACACAGTTCCTGGACCACCTTCTGACAGGCATCGAAGACAT
  CTGTGGCCACTACGGTCACCACCACTGGAAGGACAAGCTCAACCGGTTTAATAAGAAATATGTGAAGAAG
  TGTCTGATAGCTGGCGAGCGCTCCAAGGAGCCCCAGCTCATTGCCTTCTACCACAAGATGGAGATGAAGC
  AGGCCATCGAGCTGGTGGAGAGCGGGGGCATGGGCAAGATCCCCTCTGCCGTCTCCACCGTCTCCATGCA
  GAACATCCACCCCAAGTCCCTGCCTTCCGAGCGCATCCTGCCAGCACTGTCCAAGGACAAGGAGGAGGAG
  ATCCGCAAAATCCTGAGGAACAACTTGCAGAAGACCAGGCAGCGGCTGCGGTCCTACAACAGACACACGC
  TGGTGGCAGACCCCTACGAGGAAGCCTGGAACCAGATGCTGCTCCGGAGGCAGAAGGCCCGGCAGCTGGA
  GCAGAAGATCAACAACTACCTGACGGTGCCAGCCCACAAGCTGGACTCACCCACCATGTCTCGGGCCCGC
  ATCGGCTCAGACCCACTGGCCTATGAGCCGAAGGAGGACCTGCCTGTCATCACCATCGACCCGGCTTCCC
  CGCAGTCACCCGAGTCTGTGGACCTGGTGAATGAGGAGCTGAAGGGCAAAGTCTTAGGGTTGAGCCGGGA
  TCCTGCAAAGGTGGCTGAGGAGGACGAGGACGACGATGGGGGCATCATGATGCGGAGCAAGGAGACTTCG
  TCCCCAGGAACCGACGATGTCTTCACCCCCGCGCCCAGTGACAGCCCCAGCTCCCAGAGGATACAGCGCT
  GCCTCAGTGACCCAGGCCCACACCCTGAGCCTGGGGAGGGAGAACCGTTCTTCCCCAAGGGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214890 representing NM_003047
  Red=Cloning site Green=Tags(s)
  
  MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPE
  SRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGL
  LVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYA
  VCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEF
  ANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHL
  SGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLF
  CLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFF
  TVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKK
  CLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEE
  IRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRAR
  IGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETS
  SPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003047
• ORF Size: 2445 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003047.5
• RefSeq Size: 4516 bp
• RefSeq ORF: 2448 bp
• Locus ID: 6548
• UniProt ID: P19634
• Cytogenetics: 1p36.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction, Regulation of actin cytoskeleton
• MW: 90.6 kDa
• Gene Summary: This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]