RNase L (RNASEL) (NM_021133) Human Tagged ORF Clone

CAT#: RC214849

RNASEL (Myc-DDK-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL)

Product Images

Circular map for RC214849

• 

  Coomassie blue staining of purified RNASEL protein (Cat# TP314849). The protein was produced from HEK293T cells transfected with RNASEL cDNA clone (Cat# RC214849) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNase L
• Synonyms: PRCA1; RNS4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214849 representing NM_021133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAGGGATCATAACAACCCCCAGGAGGGACCCACGTCCTCCAGCGGTAGAAGGGCTGCAGTGG
  AAGACAATCACTTGCTGATTAAAGCTGTTCAAAACGAAGATGTTGACCTGGTCCAGCAATTGCTGGAAGG
  TGGAGCCAATGTTAATTTCCAGGAAGAGGAAGGGGGCTGGACACCTCTGCATAACGCAGTACAAATGAGC
  AGGGAGGACATTGTGGAACTTCTGCTTCGTCATGGTGCTGACCCTGTTCTGAGGAAGAAGAATGGGGCCA
  CGCCTTTTATCCTCGCAGCGATTGCGGGGAGCGTGAAGCTGCTGAAACTTTTCCTTTCTAAAGGAGCAGA
  TGTCAATGAGTGTGATTTTTATGGCTTCACAGCCTTCATGGAAGCCGCTGTGTATGGTAAGGTCAAAGCC
  CTAAAATTCCTTTATAAGAGAGGAGCAAATGTGAATTTGAGGCGAAAGACAAAGGAGGATCAAGAGCGGC
  TGAGGAAAGGAGGGGCCACAGCTCTCATGGACGCTGCTGAAAAAGGACACGTAGAGGTCTTGAAGATTCT
  CCTTGATGAGATGGGGGCAGATGTAAACGCCTGTGACAATATGGGCAGAAATGCCTTGATCCATGCTCTC
  CTGAGCTCTGACGATAGTGATGTGGAGGCTATTACGCATCTGCTGCTGGACCATGGGGCTGATGTCAATG
  TGAGGGGAGAAAGAGGGAAGACTCCCCTGATCCTGGCAGTGGAGAAGAAGCACTTGGGTTTGGTGCAGAG
  GCTTCTGGAGCAAGAGCACATAGAGATTAATGACACAGACAGTGATGGCAAAACAGCACTGCTGCTTGCT
  GTTGAACTCAAACTGAAGAAAATCGCCGAGTTGCTGTGCAAACGTGGAGCCAGTACAGATTGTGGGGATC
  TTGTTATGACAGCGAGGCGGAATTATGACCATTCCCTTGTGAAGGTTCTTCTCTCTCATGGAGCCAAAGA
  AGATTTTCACCCTCCTGCTGAAGACTGGAAGCCTCAGAGCTCACACTGGGGGGCAGCCCTGAAGGATCTC
  CACAGAATATACCGCCCTATGATTGGCAAACTCAAGTTCTTTATTGATGAAAAATACAAAATTGCTGATA
  CTTCAGAAGGAGGCATCTACCTGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGAAGACGTTCTGTGAGGG
  CAGCCCACGTGCACAGCGGGAAGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTC
  TATGGGAGTGAGAGCCACAGGGGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACTCTGGAAGCGT
  GTTTGGATGTGCACAGAGGGGAAGATGTGGAAAATGAGGAAGATGAATTTGCCCGAAATGTCCTGTCATC
  TATATTTAAGGCTGTTCAAGAACTACACTTGTCCTGTGGATACACCCACCAGGATCTGCAACCACAAAAC
  ATCTTAATAGATTCTAAGAAAGCTGCTCACCTGGCAGATTTTGATAAGAGCATCAAGTGGGCTGGAGATC
  CACAGGAAGTCAAGAGAGATCTAGAGGACCTTGGACGGCTGGTCCTCTATGTGGTAAAGAAGGGAAGCAT
  CTCATTTGAGGATCTGAAAGCTCAAAGTAATGAAGAGGTGGTTCAACTTTCTCCAGATGAGGAAACTAAG
  GACCTCATTCATCGTCTCTTCCATCCTGGGGAACATGTGAGGGACTGTCTGAGTGACCTGCTGGGTCATC
  CCTTCTTTTGGACTTGGGAGAGCCGCTATAGGACGCTTCGGAATGTGGGAAATGAATCCGACATCAAAAC
  ACGAAAATCTGAAAGTGAGATCCTCAGACTACTGCAACCTGGGCCTTCTGAACATTCCAAAAGTTTTGAC
  AAGTGGACGACTAAGATTAATGAATGTGTTATGAAAAAAATGAATAAGTTTTATGAAAAAAGAGGCAATT
  TCTACCAGAACACTGTGGGTGATCTGCTAAAGTTCATCCGGAATTTGGGAGAACACATTGATGAAGAAAA
  GCATAAAAAGATGAAATTAAAAATTGGAGACCCTTCCCTGTATTTTCAGAAGACATTTCCAGATCTGGTG
  ATCTATGTCTACACAAAACTACAGAACACAGAATATAGAAAGCATTTCCCCCAAACCCACAGTCCAAACA
  AGCCTCAGTGTGATGGAGCTGGTGGGGCCAGTGGGTTGGCCAGCCCTGGGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214849 representing NM_021133
  Red=Cloning site Green=Tags(s)
  
  MESRDHNNPQEGPTSSSGRRAAVEDNHLLIKAVQNEDVDLVQQLLEGGANVNFQEEEGGWTPLHNAVQMS
  REDIVELLLRHGADPVLRKKNGATPFILAAIAGSVKLLKLFLSKGADVNECDFYGFTAFMEAAVYGKVKA
  LKFLYKRGANVNLRRKTKEDQERLRKGGATALMDAAEKGHVEVLKILLDEMGADVNACDNMGRNALIHAL
  LSSDDSDVEAITHLLLDHGADVNVRGERGKTPLILAVEKKHLGLVQRLLEQEHIEINDTDSDGKTALLLA
  VELKLKKIAELLCKRGASTDCGDLVMTARRNYDHSLVKVLLSHGAKEDFHPPAEDWKPQSSHWGAALKDL
  HRIYRPMIGKLKFFIDEKYKIADTSEGGIYLGFYEKQEVAVKTFCEGSPRAQREVSCLQSSRENSHLVTF
  YGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFARNVLSSIFKAVQELHLSCGYTHQDLQPQN
  ILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDLGRLVLYVVKKGSISFEDLKAQSNEEVVQLSPDEETK
  DLIHRLFHPGEHVRDCLSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFD
  KWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLV
  IYVYTKLQNTEYRKHFPQTHSPNKPQCDGAGGASGLASPGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021133
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021133.4
• RefSeq Size: 4258 bp
• RefSeq ORF: 2226 bp
• Locus ID: 6041
• UniProt ID: Q05823
• Cytogenetics: 1q25.3
• Domains: pkinase, ANK, S_TKc, PUG
• Protein Families: Druggable Genome, Protein Kinase
• MW: 83.5 kDa
• Gene Summary: This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]