RNase L (RNASEL) (NM_021133) Human Untagged Clone

CAT#: SC323686

RNASEL (untagged)-Kinase deficient mutant (K392M) of Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNase L
• Synonyms: PRCA1; RNS4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323686 sequence for NM_021133 edited (data generated by NextGen Sequencing)
  ATGGAGAGCAGGGATCATAACAACCCCCAGGAGGGACCCACGTCCTCCAGCGGTAGAAGG
  GCTGCAGTGGAAGACAATCACTTGCTGATTAAAGCTGTTCAAAACGAAGATGTTGACCTG
  GTCCAGCAATTGCTGGAAGGTGGAGCCAATGTTAATTTCCAGGAAGAGGAAGGGGGCTGG
  ACACCTCTGCATAACGCAGTACAAATGAGCAGGGAGGACATTGTGGAACTTCTGCTTCGT
  CATGGTGCTGACCCTGTTCTGAGGAAGAAGAATGGGGCCACGCCTTTTATCCTCGCAGCG
  ATTGCGGGGAGCGTGAAGCTGCTGAAACTTTTCCTTTCTAAAGGAGCAGATGTCAATGAG
  TGTGATTTTTATGGCTTCACAGCCTTCATGGAAGCCGCTGTGTATGGTAAGGTCAAAGCC
  CTAAAATTCCTTTATAAGAGAGGAGCAAATGTGAATTTGAGGCGAAAGACAAAGGAGGAT
  CAAGAGCGGCTGAGGAAAGGAGGGGCCACAGCTCTCATGGACGCTGCTGAAAAAGGACAC
  GTAGAGGTCTTGAAGATTCTCCTTGATGAGATGGGGGCAGATGTAAACGCCTGTGACAAT
  ATGGGCAGAAATGCCTTGATCCATGCTCTCCTGAGCTCTGACGATAGTGATGTGGAGGCT
  ATTACGCATCTGCTGCTGGACCATGGGGCTGATGTCAATGTGAGGGGAGAAAGAGGGAAG
  ACTCCCCTGATCCTGGCAGTGGAGAAGAAGCACTTGGGTTTGGTGCAGAGGCTTCTGGAG
  CAAGAGCACATAGAGATTAATGACACAGACAGTGATGGCAAAACAGCACTGCTGCTTGCT
  GTTGAACTCAAACTGAAGAAAATCGCCGAGTTGCTGTGCAAACGTGGAGCCAGTACAGAT
  TGTGGGGATCTTGTTATGACAGCGAGGCGGAATTATGACCATTCCCTTGTGAAGGTTCTT
  CTCTCTCATGGAGCCAAAGAAGATTTTCACCCTCCTGCTGAAGACTGGAAGCCTCAGAGC
  TCACACTGGGGGGCAGCCCTGAAGGATCTCCACAGAATATACCGCCCTATGATTGGCAAA
  CTCAAGTTCTTTATTGATGAAAAATACAAAATTGCTGATACTTCAGAAGGAGGCATCTAC
  CTGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGATGACGTTCTGTGAGGGCAGCCCACGT
  GCACAGCGGGAAGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTC
  TATGGGAGTGAGAGCCACAGGGGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACT
  CTGGAAGCGTGTTTGGATGTGCACAGAGGGGAAGATGTGGAAAATGAGGAAGATGAATTT
  GCCCGAAATGTCCTGTCATCTATATTTAAGGCTGTTCAAGAACTACACTTGTCCTGTGGA
  TACACCCACCAGGATCTGCAACCACAAAACATCTTAATAGATTCTAAGAAAGCTGCTCAC
  CTGGCAGATTTTGATAAGAGCATCAAGTGGGCTGGAGATCCACAGGAAGTCAAGAGAGAT
  CTAGAGGACCTTGGACGGCTGGTCCTCTATGTGGTAAAGAAGGGAAGCATCTCATTTGAG
  GATCTGAAAGCTCAAAGTAATGAAGAGGTGGTTCAACTTTCTCCAGATGAGGAAACTAAG
  GACCTCATTCATCGTCTCTTCCATCCTGGGGAACATGTGAGGGACTGTCTGAGTGACCTG
  CTGGGTCATCCCTTCTTTTGGACTTGGGAGAGCCGCTATAGGACGCTTCGGAATGTGGGA
  AATGAATCCGACATCAAAACACGAAAATCTGAAAGTGAGATCCTCAGACTACTGCAACCT
  GGGCCTTCTGAACATTCCAAAATTTTTGACAAGTGGACGACTAAGATTAATGAATGTGTT
  ATGAAAAAAATGAATAAGTTTTATGAAAAAAGAGGCAATTTCTACCAGAACACTGTGGGT
  GATCTGCTAAAGTTCATCCGGAATTTGGGAGAACACATTGATGAAGAAAAGCATAAAAAG
  ATGAAATTAAAAATTGGAGACCCTTCCCTGTATTTTCAGAAGACATTTCCAGATCTGGTG
  ATCTATGTCTACACAAAACTACAGAACACAGAATATAGAAAGCATTTCCCCCAAACCCAC
  AGTCCAAACAAGCCTCAGTGTGATGGAGCTGGTGGGGCCAGTGGGTTGGCCAGCCCTGGG
  TGCTGA
  
  Clone variation with respect to NM_021133.3
  1175 a=>t;1883 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_021133 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGCTCGGGCTGCAA
  GCAGTCTTCCAGGCTTTGCGGCTGCCAAAGGAATAATTGAGACGTCTGAGTTGAGCAGGTGGAATGTCAG
  AAGACTGAGAACATTGTTCCTTCTTCATACTGCTGCTCTGTTGCCAGAGAATCCCAATTTACACTCAAAG
  CTTCTTTGATTAAGTGCTAGGAGATAAATTTGCATTTTCTCAAGGAAAAGGCTAAAAGTGGTAGCAGGGT
  GGCATTTACCGTCATGGGAGAGCAGGGATCATAACAACCCCCCAGGAAGGGACCCACGTCCTTCCAGCGG
  TAGAAGGGCTGCAGTGGAAGACAATCACTTGCTGATTAAAGCTGGTTCAAAACGAAGAATGTTGACTTGT
  TCAGCATTGCTGGGAGGTGGAGCAAAGGTAAATTTCAGAAAAAGGAAGGGGCCTGGACCCCCCCCATTAA
  GGGATTCCAATGGGCGAGGGAGGACTTTGGAATTTTCCTCTGGCAAGGTGCCTGACCGGTTCTAGAGAAG
  AGATGGGCCACGCTTTTACTCTCACAGATTCGGGAGCCGTAACTCTCGAACCTTCCTTTAAGAGACAATG
  CAGAGGGAATTTAGGCCAACTTTGGACCCTGTGTTGAAGCAACCTAATCCTTAAAGCATGAATAGCCACA
  GGATCAGGTGAAAGGCCACCTAGCTCGAAGACTTAGCTGATCTGAATTGCAATACCGGTAATGCAATGCT
  TCGCTGCTTCAAGTGAGCTC
  
  Kinase domain sequence

  >SC323686 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CWTGATTGGCACTCAGTTCTTTATTGATGAAAATACAAAATTGCTGATACTTCAGAAGGAGGCATCTACC
  TGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGATGACGTTCTGTGAGGGCAGCCCACGTGCACAGCGGGA
  AGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTCTATGGGAGTGAGAGCCACAGG
  GGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACTCTGGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_021133
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021133.2, NP_066956.1
• RefSeq Size: 4166 bp
• RefSeq ORF: 2226 bp
• Locus ID: 6041
• UniProt ID: Q05823
• Cytogenetics: 1q25.3
• Domains: pkinase, ANK, S_TKc, PUG
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]