SLC22A17 (NM_016609) Human Tagged ORF Clone

CAT#: RC214220

SLC22A17 (Myc-DDK-tagged)-Human solute carrier family 22, member 17 (SLC22A17), transcript variant 2

Product Images

Circular map for RC214220

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC22A17 (Cat# RC214220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC22A17 (Cat# TA810195)(1:500). Positive lysates LY413864 (100ug) and LC413864 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413864) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214220 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC22A17
• Synonyms: 24p3R; BOCT; BOIT; hBOIT; NGALR; NGALR2; NGALR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214220 representing NM_016609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGACCCCATCTTCACGCTGGCGCCCCCGCTGCATTGCCACTACGGGGCCTTCCCCCCTAATG
  CCTCTGGCTGGGAGCAGCCTCCCAATGCCAGCGGCGTCAGCGTCGCCAGCGCTGCCCTAGCAGCCAGCGC
  CGCCAGCCGTGTCGCCACCAGTACCGACCCCTCGTGCAGCGGCTTCGCCCCGCCGGACTTCAACCATTGC
  CTCAAGGATTGGGACTATAATGGCCTTCCTGTGCTCACCACCAACGCCATCGGCCAGTGGGATCTGGTGT
  GTGACCTGGGCTGGCAGGTGATCCTGGAGCAGATCCTCTTCATCTTGGGCTTTGCCTCCGGCTACCTGTT
  CCTGGGTTACCCCGCAGACAGATTTGGCCGTCGCGGGATTGTGCTGCTGACCTTGGGGCTGGTGGGCCCC
  TGTGGAGTAGGAGGGGCTGCTGCAGGCTCCTCCACAGGCGTCATGGCCCTCCGATTCCTCTTGGGCTTTC
  TGCTTGCCGGTGTTGACCTGGGTGTCTACCTGATGCGCCTGGAGCTGTGCGACCCAACCCAGAGGCTTCG
  GGTGGCCCTGGCAGGGGAGTTGGTGGGGGTGGGAGGGCACTTCCTGTTCCTGGGCCTGGCCCTTGTCTCT
  AAGGATTGGCGATTCCTACAGCGAATGATCACCGCTCCCTGCATCCTCTTCCTGTTTTATGGCTGGCCTG
  GTTTGTTCCTGGAGTCCGCACGGTGGCTGATAGTGAAGCGGCAGATTGAGGAGGCTCAGTCTGTGCTGAG
  GATCCTGGCTGAGCGAAACCGGCCCCATGGGCAGATGCTGGGGGAGGAGGCCCAGGAGGCCCTGCAGGAC
  CTGGAGAATACCTGCCCTCTCCCTGCAACATCCTCCTTTTCCTTTGCTTCCCTCCTCAACTACCGCAACA
  TCTGGAAAAATCTGCTTATCCTGGGCTTCACCAACTTCATTGCCCATGCCATTCGCCACTGCTACCAGCC
  TGTGGGAGGAGGAGGGAGCCCATCGGACTTCTACCTGTGCTCTCTGCTGGCCAGCGGCACCGCAGCCCTG
  GCCTGTGTCTTCCTGGGGGTCACCGTGGACCGATTTGGCCGCCGGGGCATCCTTCTTCTCTCCATGACCC
  TTACCGGCATTGCTTCCCTGGTCCTGCTGGGCCTGTGGGATTATCTGAACGAGGCTGCCATCACCACTTT
  CTCTGTCCTTGGGCTCTTCTCCTCCCAAGCTGCCGCCATCCTCAGCACCCTCCTTGCTGCTGAGGTCATC
  CCCACCACTGTCCGGGGCCGTGGCCTGGGCCTGATCATGGCTCTAGGGGCGCTTGGAGGACTGAGCGGCC
  CGGCCCAGCGCCTCCACATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGCGCCCTCCT
  CTGCATTCTCAGCATTATGCTGCTGCCGGAGACCAAGCGCAAGCTCCTGCCCGAGGTGCTCCGGGACGGG
  GAGCTGTGTCGCCGGCCTTCCCTGCTGCGGCAGCCACCCCCTACCCGCTGTGACCACGTCCCGCTGCTTG
  CCACCCCCAACCCTGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214220 representing NM_016609
  Red=Cloning site Green=Tags(s)
  
  MASDPIFTLAPPLHCHYGAFPPNASGWEQPPNASGVSVASAALAASAASRVATSTDPSCSGFAPPDFNHC
  LKDWDYNGLPVLTTNAIGQWDLVCDLGWQVILEQILFILGFASGYLFLGYPADRFGRRGIVLLTLGLVGP
  CGVGGAAAGSSTGVMALRFLLGFLLAGVDLGVYLMRLELCDPTQRLRVALAGELVGVGGHFLFLGLALVS
  KDWRFLQRMITAPCILFLFYGWPGLFLESARWLIVKRQIEEAQSVLRILAERNRPHGQMLGEEAQEALQD
  LENTCPLPATSSFSFASLLNYRNIWKNLLILGFTNFIAHAIRHCYQPVGGGGSPSDFYLCSLLASGTAAL
  ACVFLGVTVDRFGRRGILLLSMTLTGIASLVLLGLWDYLNEAAITTFSVLGLFSSQAAAILSTLLAAEVI
  PTTVRGRGLGLIMALGALGGLSGPAQRLHMGHGAFLQHVVLAACALLCILSIMLLPETKRKLLPEVLRDG
  ELCRRPSLLRQPPPTRCDHVPLLATPNPAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016609
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016609.4, NP_057693.3
• RefSeq Size: 2344 bp
• RefSeq ORF: 1563 bp
• Locus ID: 51310
• UniProt ID: Q8WUG5
• Cytogenetics: 14q11.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 55.5 kDa
• Gene Summary: Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]