GCNT1 (NM_001490) Human Tagged ORF Clone

CAT#: RC214131

GCNT1 (Myc-DDK-tagged)-Human glucosaminyl (N-acetyl) transferase 1, core 2 (GCNT1), transcript variant 2

Product Images

Circular map for RC214131

• 

  Western blot validation of overexpression lysate (Cat# LY420411) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224441 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GCNT1
• Synonyms: C2GNT; C2GNT-L; C2GNT1; G6NT; NACGT2; NAGCT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214131 representing NM_001490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGACGTTGCTGCGAAGGAGACTTTTTTCTTATCCCACCAAATACTACTTTATGGTTCTTGTTT
  TATCCCTAATCACCTTCTCCGTTTTAAGGATTCATCAAAAGCCTGAATTTGTAAGTGTCAGACACTTGGA
  GCTTGCTGGGGAGAATCCTAGTAGTGATATTAATTGCACCAAAGTTTTACAGGGTGATGTAAATGAAATC
  CAAAAGGTAAAGCTTGAGATCCTAACAGTGAAATTTAAAAAGCGCCCTCGGTGGACACCTGACGACTATA
  TAAACATGACCAGTGACTGTTCTTCTTTCATCAAGAGACGCAAATATATTGTAGAACCCCTTAGTAAAGA
  AGAGGCGGAGTTTCCAATAGCATATTCTATAGTGGTTCATCACAAGATTGAAATGCTTGACAGGCTGCTG
  AGGGCCATCTATATGCCTCAGAATTTCTATTGCATTCATGTGGACACAAAATCCGAGGATTCCTATTTAG
  CTGCAGTGATGGGCATCGCTTCCTGTTTTAGTAATGTCTTTGTGGCCAGCCGATTGGAGAGTGTGGTTTA
  TGCATCGTGGAGCCGGGTTCAGGCTGACCTCAACTGCATGAAGGATCTCTATGCAATGAGTGCAAACTGG
  AAGTACTTGATAAATCTTTGTGGTATGGATTTTCCCATTAAAACCAACCTAGAAATTGTCAGGAAGCTCA
  AGTTGTTAATGGGAGAAAACAACCTGGAAACGGAGAGGATGCCATCCCATAAAGAAGAAAGGTGGAAGAA
  GCGGTATGAGGTCGTTAATGGAAAGCTGACAAACACAGGGACTGTCAAAATGCTTCCTCCACTCGAAACA
  CCTCTCTTTTCTGGCAGTGCCTACTTCGTGGTCAGTAGGGAGTATGTGGGGTATGTACTACAGAATGAAA
  AAATCCAAAAGTTGATGGAGTGGGCACAAGACACATACAGCCCTGATGAGTATCTCTGGGCCACCATCCA
  AAGGATTCCTGAAGTCCCGGGCTCACTCCCTGCCAGCCATAAGTATGATCTGTCTGACATGCAAGCAGTT
  GCCAGGTTTGTCAAGTGGCAGTACTTTGAGGGTGATGTTTCCAAGGGTGCTCCCTACCCGCCCTGCGATG
  GAGTCCATGTGCGCTCAGTGTGCATTTTCGGAGCTGGTGACTTGAACTGGATGCTGCGCAAACACCACTT
  GTTTGCCAATAAGTTTGACGTGGATGTTGACCTCTTTGCCATCCAGTGTTTGGATGAGCATTTGAGACAC
  AAAGCTTTGGAGACATTAAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214131 representing NM_001490
  Red=Cloning site Green=Tags(s)
  
  MLRTLLRRRLFSYPTKYYFMVLVLSLITFSVLRIHQKPEFVSVRHLELAGENPSSDINCTKVLQGDVNEI
  QKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLL
  RAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANW
  KYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLET
  PLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAV
  ARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRH
  KALETLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001490
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001490.5
• RefSeq Size: 5536 bp
• RefSeq ORF: 1287 bp
• Locus ID: 2650
• UniProt ID: Q02742
• Cytogenetics: 9q21.13
• Domains: Branch
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 49.6 kDa
• Gene Summary: This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]