GCNT1 (NM_001490) Human Untagged Clone

CAT#: SC110905

GCNT1 (untagged)-Human glucosaminyl (N-acetyl) transferase 1, core 2 (GCNT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCNT1
• Synonyms: C2GNT; C2GNT-L; C2GNT1; G6NT; NACGT2; NAGCT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110905 sequence for NM_001490 edited (data generated by NextGen Sequencing)
  ATGCTGAGGACGTTGCTGCGAAGGAGACTTTTTTCTTATCCCACCAAATACTACTTTATG
  GTTCTTGTTTTATCCCTAATCACCTTCTCCGTTTTAAGGATTCATCAAAAGCCTGAATTT
  GTAAGTGTCAGACACTTGGAGCTTGCTGGGGAGAATCCTAGTAGTGATATTAATTGCACC
  AAAGTTTTACAGGGTGATGTAAATGAAATCCAAAAGGTAAAGCTTGAGATCCTAACAGTG
  AAATTTAAAAAGCGCCCTCGGTGGACACCTGACGACTATATAAACATGACCAGTGACTGT
  TCTTCTTTCATCAAGAGACGCAAATATATTGTAGAACCCCTTAGTAAAGAAGAGGCGGAG
  TTTCCAATAGCATATTCTATAGTGGTTCATCACAAGATTGAAATGCTTGACAGGCTGCTG
  AGGGCCATCTATATGCCTCAGAATTTCTATTGCATTCATGTGGACACAAAATCCGAGGAT
  TCCTATTTAGCTGCAGTGATGGGCATCGCTTCCTGTTTTAGTAATGTCTTTGTGGCCAGC
  CGATTGGAGAGTGTGGTTTATGCATCGTGGAGCCGGGTTCAGGCTGACCTCAACTGCATG
  AAGGATCTCTATGCAATGAGTGCAAACTGGAAGTACTTGATAAATCTTTGTGGTATGGAT
  TTTCCCATTAAAACCAACCTAGAAATTGTCAGGAAGCTCAAGTTGTTAATGGGAGAAAAC
  AACCTGGAAACGGAGAGGATGCCATCCCATAAAGAAGAAAGGTGGAAGAAGCGGTATGAG
  GTCGTTAATGGAAAGCTGACAAACACAGGGACTGTCAAAATGCTTCCTCCACTCGAAACA
  CCTCTCTTTTCTGGCAGTGCCTACTTCGTGGTCAGTAGGGAGTATGTGGGGTATGTACTA
  CAGAATGAAAAAATCCAAAAGTTGATGGAGTGGGCACAAGACACATACAGCCCTGATGAG
  TATCTCTGGGCCACCATCCAAAGGATTCCTGAAGTCCCGGGCTCACTCCCTGCCAGCCAT
  AAGTATGATCTGTCTGACATGCAAGCAGTTGCCAGGTTTGTCAAGTGGCAGTACTTTGAG
  GGTGATGTTTCCAAGGGTGCTCCCTACCCGCCCTGCGATGGAGTCCATGTGCGCTCAGTG
  TGCATTTTCGGAGCTGGTGACTTGAACTGGATGCTGCGCAAACACCACTTGTTTGCCAAT
  AAGTTTGACGTGGATGTTGACCTCTTTGCCATCCAGTGTTTGGATGAGCATTTGAGACAC
  AAAGCTTTGGAGACATTAAAACACTGA
  
  Clone variation with respect to NM_001490.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001490 unedited
  GCAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGGGGCG
  GGAGCTTGGTGGAGCAGGAGCGGCTGGGCATCCTCCTGAGACTCCGGGGTCAGACGCCCA
  CTCCAGCGCTTTCTGCGCAGCACAGGCACCCTCGCCTGTCACGCCTCCCTCTCCTCTTCC
  CGTCTCCCAGCGTGGATGAGGGCGCCTGCAGATCCCGGCTAGGGCACGGACCGCCTAGGC
  CGAGATTTCTTTAAAGCCTCGTGCAGCACATCATTATCGCTGGATGCCCGGACATGTAAT
  ACACCTGACAGCATGTGAAGTGCTCAGAATGGGGCAGGATGTCACCTGGAATCAGCACTA
  AGTGATTCAGACTTTCCTTACTTTTAAATGGGCTGCTGACACCAGCTAATGGACATTAAA
  ACATGTCAGGAGGAACTTAAATTACTAACGATTTCAAGACAAGGGGTGGTAATTCCGGCA
  CAGTGTCAGAAACTCATCAACTATAGACTGTTCAATCATTATTGCCTAGGCGTCCAACTA
  TGGTTTTGCACCTCAACATACCAAAGAGTGCTGCTCTTCATTTCAAGATGCCGTTGCAGC
  TCTGATAAATGCAAACTGACAACCTTCAAGGCCACGACGGAGGGAAAATCATTGGTGCTT
  GGAGCATAGAAGACTGCCCTTCACAAAGGAAATCCCTGATTATTGTTTGAAATGCTGAGG
  ACGTTGCTGCGAAGGAGACTNTNTTCTTATCCCACCAAATACTACTNTATGGGNTCTTGG
  TTTATCCCTAATCACCTTCCTCCGTTNTAAGGATTCATCANAAGCCTGAAATTGTAAGTG
  TCAGACACTTGGAGCTTGCTGGNGAGAAATCTAGTAGTGATATTAATTGCACCAAAGTTT
  TACAGGGTGATGTAAATGAAATCCAAAGGGTAAGCTGGAGATCCCTACAGTGAAATTAAA
  AGCGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001490 unedited
  GGCACGCTTCTAGNGACGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTAAAATAAACCCCATTTTAAGCCCCCGGGGAGGACATACGG
  GGACCCCCTACCCAGGCCAGTTTTAGAGCAAAATATAAATTTTTTACAAAAATCTTTTAT
  GGGACAATCAAATTTTTTTTTCAACAAATATAATACCAGATTAATGGGTTCTTGGATTAT
  TGGGGGGGGGAAGTTTATTCATCCCAAAAAAAGGGGCACATTTAAAATTCTAAAAAAAGT
  TGGGGGACAGGGAACATTTTCACATTTGGGTAAGCCCCGGAGCACTTTGAACAGGGGGAT
  TTTATGGATGGGCCTTTTCCCAAGCCACCCGGGGCCCCTTTTTCCAAAAGGCCTTGGTAC
  CGGGTTGGGCCTAAAGGGTACCTTTACTCCCCCCACTATTAACTCTAAAGGAACATTTAA
  GGCTATCACCTTTCTAGCTAACGGGGCTCGGGAAAAACCAGGCAGCTTCTCTGACAAAAA
  AATCTACTAAAGGCCCGGCCCCAAAGAGGACTTTATACCATTTTCCCGACGCTGACAGGG
  AAGGGGAAACAGATAGGGGGCCGTTTGGGGGACCCTTCTTCTGGCCATAAAATGGCCCGA
  AAGGGCCAGGGTTTTAAGGGCTCCAAAGCTTTGGGGCTAAAAGGCTCATCCAAACACTGG
  ATGGCNAAGAGGGCAACATCCACGTCAAACTTATTGCAAAACAGGGGTGTTGGGGCACAT
  TCAGTTAAAGTCCCAGCTCCGAAAATGCCACTGAGCGCCCATGAATCTCCTCGCAGGGGG
  GGTAGGGAGCCCCNCTGGAAACATCCCCTAAAAGACTGGCCCTTGAAAACCGGGCACTGG
  TTTATGGAAAACGATATACTTAGGCGTGAAGGGAGGAGCCGGAACTCAGGAATCTTGGTA
  GGGGNCCAAATACTAAAGGGCTGGTTGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001490
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001490.3, NP_001481.2
• RefSeq Size: 5536 bp
• RefSeq ORF: 1287 bp
• Locus ID: 2650
• UniProt ID: Q02742
• Cytogenetics: 9q21.13
• Domains: Branch
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1, and uses the P2 promoter.