PRDM13 (NM_021620) Human Tagged ORF Clone

CAT#: RC214016

PRDM13 (Myc-DDK-tagged)-Human PR domain containing 13 (PRDM13)

Product Images

Circular map for RC214016

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRDM13
• Synonyms: MU-MB-20.220; PFM10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214016 representing NM_021620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGAGCCGCCAGAGCGCCAGCCACCAGCGTGAGTGCCGACTGCTGCATCCCGGCCGGCTTGCGCC
  TCGGACCGGTGCCTGGTACCTTCAAGCTGGGCAAGTACCTGTCAGACCGCAGGGAGCCCGGGCCTAAGAA
  AAAGGTGCGCATGGTGAGAGGGGAGCTGGTGGACGAGTCGGGGGGCTCCCCTCTGGAGTGGATAGGGTTA
  ATCCGGGCAGCCAGAAACTCCCAGGAACAGACTCTGGAAGCTATTGCAGACTTACCCGGAGGACAGATCT
  TCTACCGAGCATTGCGAGACGTCCAGCCAGGGGAGGAGCTGACAGTGTGGTATTCTAACTCCTTGGCTCA
  GTGGTTCGACATCCCCACCACAGCGACTCCGACTCACGACGAGAAAGGGGAGGAGCGCTACATCTGCTGG
  TACTGCTGGAGGACGTTTAGATACCCCAACAGCCTTAAGGCACACCTGCGTTTCCACTGCGTGTTCAGCG
  GCGGTGGAGGCGGCGCCTTCCTGCACCACGAACACGCGGCTCGCCAAGGCGCCGTCCCAGCGGCTGATGG
  CCTCGGTCTCTCCCCAAAACCCCCGGCGCCCGATTTCGCCGCGCCTTCCCAGGCAGGAACTTTGCGACCC
  CACCCCCTGGGCCCGCCACCAGTTCAGGCCTGCGGTGCGCGGGAGGGCATCAAGCGCGAGGCCTCTTCCG
  CGCCCTCGGCCACCTCGCCGACCCCAGGCAAGTGGGGGCAGCCCAAGAAGGGCAAGGAGCAGCTGGACCG
  TGCCCTGGACATGAGCGGAGCCGCCCGAGGACAAGGGCACTTCCTCGGCATCGTGGGCGGCTCCTCGGCG
  GGGGTCGGCAGCCTGGCTTTCTACCCCGGCGTGCGCTCAGCTTTCAAGCCCGCCGGCCTAGCGAGGGCGG
  CGGCGGCCGCTCACGGCGACCCCTACCGGGAGGAGAGCAGCAGCAAGCAAGGAGCCGGCCTCGCTTTGGG
  CAGGCTGCTGGGCGGGGGCCGGGCGTGCGGGCGCCCCGGGAGCGGGGAGAACTCGGCGGCGGGCGGCGCG
  GGTCACCACCATCACCACCACGCGCACCACCACCACCATCCCAAGTGCCTGCTCGCTGGGGACCCGCCGC
  CGCCGCCGCCGCCTGGCCTGCCCTGCTCTGGGGCCCTGCGCGGCTTCCCTCTGCTCTCCGTCCCCCCGGA
  AGAGGCGTCCGCCTTCAAGCACGTGGAGCGCGCCCCGCCCGCAGCCGCCGCGCTGCCAGGAGCGCGTTAT
  GCGCAGCTGCCCCCTGCGCCGGGGTTGCCCCTCGAGCGCTGCGCGCTGCCGCCCCTCGACCCGGGCGGTC
  TCAAAGCCTATCCGGGTGGTGAGTGCAGCCACCTGCCCGCCGTCATGCCGGCCTTTACAGTCTACAACGG
  GGAGCTGCTCTACGGCTCACCGGCCACCACCGCTTATTACCCGCTCAAATTGCACTTCGGCGGGCTGCTG
  AAGTATCCGGAGTCCATCTCCTACTTCAGCGGGCCTGCAGCGGCCGCCCTAAGCCCCGCCGAGCTGGGGT
  CGCTGGCCAGCATCGACCGAGAGATCGCCATGCACAATCAGCAGCTGTCCGAGATGGCTGCCGGGAAGGG
  TCGCGGACGCCTGGACTCGGGGACGTTGCCACCGGCCGTCGCGGCGGCGGGAGGCACCGGGGGCGGCGGC
  AGCGGAGGCAGCGGCGCAGGTAAGCCCAAGACCGGCCACCTGTGCCTCTACTGTGGCAAGCTGTACTCGC
  GCAAGTATGGGCTCAAGATCCACATGCGGACGCACACGGGCTACAAGCCACTCAAGTGCAAAGTCTGTCT
  GCGGCCCTTCGGCGACCCCAGCAATCTCAACAAGCACATCCGGCTGCACGCCGAGGGCAATACGCCCTAC
  CGCTGCGAGTTCTGCGGCAAGGTACTTGTGCGCCGCCGGGACCTGGAGCGACATGTCAAGTCCCGCCACC
  CTGGCCAGAGTCTGCTCGCCAAAGCGGGCGACGGCCCGGGTGCCGAGCCCGGCTATCCCCCGGAGCCTGG
  GGATCCCAAGAGCGACGACAGTGACGTGGACGTCTGCTTCACAGACGACCAGAGCGACCCCGAGGTTGGG
  GGCGGCGGGGAGCGCGACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214016 representing NM_021620
  Red=Cloning site Green=Tags(s)
  
  MHGAARAPATSVSADCCIPAGLRLGPVPGTFKLGKYLSDRREPGPKKKVRMVRGELVDESGGSPLEWIGL
  IRAARNSQEQTLEAIADLPGGQIFYRALRDVQPGEELTVWYSNSLAQWFDIPTTATPTHDEKGEERYICW
  YCWRTFRYPNSLKAHLRFHCVFSGGGGGAFLHHEHAARQGAVPAADGLGLSPKPPAPDFAAPSQAGTLRP
  HPLGPPPVQACGAREGIKREASSAPSATSPTPGKWGQPKKGKEQLDRALDMSGAARGQGHFLGIVGGSSA
  GVGSLAFYPGVRSAFKPAGLARAAAAAHGDPYREESSSKQGAGLALGRLLGGGRACGRPGSGENSAAGGA
  GHHHHHHAHHHHHPKCLLAGDPPPPPPPGLPCSGALRGFPLLSVPPEEASAFKHVERAPPAAAALPGARY
  AQLPPAPGLPLERCALPPLDPGGLKAYPGGECSHLPAVMPAFTVYNGELLYGSPATTAYYPLKLHFGGLL
  KYPESISYFSGPAAAALSPAELGSLASIDREIAMHNQQLSEMAAGKGRGRLDSGTLPPAVAAAGGTGGGG
  SGGSGAGKPKTGHLCLYCGKLYSRKYGLKIHMRTHTGYKPLKCKVCLRPFGDPSNLNKHIRLHAEGNTPY
  RCEFCGKVLVRRRDLERHVKSRHPGQSLLAKAGDGPGAEPGYPPEPGDPKSDDSDVDVCFTDDQSDPEVG
  GGGERDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021620
• ORF Size: 2121 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021620.4
• RefSeq Size: 3204 bp
• RefSeq ORF: 2124 bp
• Locus ID: 59336
• UniProt ID: Q9H4Q3
• Cytogenetics: 6q16.2
• MW: 74.4 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]