PRDM13 (NM_021620) Human Tagged ORF Clone

CAT#: RG214016

PRDM13 (tGFP-tagged) - Human PR domain containing 13 (PRDM13)

Product Images

Circular map for RG214016

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRDM13
• Synonyms: MU-MB-20.220; PFM10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214016 representing NM_021620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGAGCCGCCAGAGCGCCAGCCACCAGCGTGAGTGCCGACTGCTGCATCCCGGCCGGCTTGCGCC
  TCGGACCGGTGCCTGGTACCTTCAAGCTGGGCAAGTACCTGTCAGACCGCAGGGAGCCCGGGCCTAAGAA
  AAAGGTATTGACCATTCAGACCTCTGCCCACCAGGTGCGCATGGTGAGAGGGGAGCTGGTGGACGAGTCG
  GGGGGCTCCCCTCTGGAGTGGATAGGGTTAATCCGGGCAGCCAGAAACTCCCAGGAACAGACTCTGGAAG
  CTATTGCAGACTTACCCGGAGGACAGATCTTCTACCGAGCATTGCGAGACGTCCAGCCAGGGGAGGAGCT
  GACAGTGTGGTATTCTAACTCCTTGGCTCAGTGGTTCGACATCCCCACCACAGCGACTCCGACTCACGAC
  GAGAAAGGGGAGGAGCGCTACATCTGCTGGTACTGCTGGAGGACGTTTAGATACCCCAACAGCCTTAAGG
  CACACCTGCGTTTCCACTGCGTGTTCAGCGGCGGTGGAGGCGGCGCCTTCCTGCACCACGAACACGCGGC
  TCGCCAAGGCGCCGTCCCAGCGGCTGATGGCCTCGGTCTCTCCCCAAAACCCCCGGCGCCCGATTTCGCC
  GCGCCTTCCCAGGCAGGAACTTTGCGACCCCACCCCCTGGGCCCGCCACCAGTTCAGGCCTGCGGTGCGC
  GGGAGGGCATCAAGCGCGAGGCCTCTTCCGCGCCCTCGGCCACCTCGCCGACCCCAGGCAAGTGGGGGCA
  GCCCAAGAAGGGCAAGGAGCAGCTGGACCGTGCCCTGGACATGAGCGGAGCCGCCCGAGGACAAGGGCAC
  TTCCTCGGCATCGTGGGCGGCTCCTCGGCGGGGGTCGGCAGCCTGGCTTTCTACCCCGGCGTGCGCTCAG
  CTTTCAAGCCCGCCGGCCTAGCGAGGGCGGCGGCGGCCGCTCACGGCGACCCCTACCGGGAGGAGAGCAG
  CAGCAAGCAAGGAGCCGGCCTCGCTTTGGGCAGGCTGCTGGGCGGGGGCCGGGCGTGCGGGCGCCCCGGG
  AGCGGGGAGAACTCGGCGGCGGGCGGCGCGGGTCACCACCATCACCACCACGCGCACCACCACCACCATC
  CCAAGTGCCTGCTCGCTGGGGACCCGCCGCCGCCGCCGCCGCCTGGCCTGCCCTGCTCTGGGGCCCTGCG
  CGGCTTCCCTCTGCTCTCCGTCCCCCCGGAAGAGGCGTCCGCCTTCAAGCACGTGGAGCGCGCCCCGCCC
  GCAGCCGCCGCGCTGCCAGGAGCGCGTTATGCGCAGCTGCCCCCTGCGCCGGGGTTGCCCCTCGAGCGCT
  GCGCGCTGCCGCCCCTCGACCCGGGCGGTCTCAAAGCCTATCCGGGTGGTGAGTGCAGCCACCTGCCCGC
  CGTCATGCCGGCCTTTACAGTCTACAACGGGGAGCTGCTCTACGGCTCACCGGCCACCACCGCTTATTAC
  CCGCTCAAATTGCACTTCGGCGGGCTGCTGAAGTATCCGGAGTCCATCTCCTACTTCAGCGGGCCTGCAG
  CGGCCGCCCTAAGCCCCGCCGAGCTGGGGTCGCTGGCCAGCATCGACCGAGAGATCGCCATGCACAATCA
  GCAGCTGTCCGAGATGGCTGCCGGGAAGGGTCGCGGACGCCTGGACTCGGGGACGTTGCCACCGGCCGTC
  GCGGCGGCGGGAGGCACCGGGGGCGGCGGCAGCGGAGGCAGCGGCGCAGGTAAGCCCAAGACCGGCCACC
  TGTGCCTCTACTGTGGCAAGCTGTACTCGCGCAAGTATGGGCTCAAGATCCACATGCGGACGCACACGGG
  CTACAAGCCACTCAAGTGCAAAGTCTGTCTGCGGCCCTTCGGCGACCCCAGCAATCTCAACAAGCACATC
  CGGCTGCACGCCGAGGGCAATACGCCCTACCGCTGCGAGTTCTGCGGCAAGGTACTTGTGCGCCGCCGGG
  ACCTGGAGCGACATGTCAAGTCCCGCCACCCTGGCCAGAGTCTGCTCGCCAAAGCGGGCGACGGCCCGGG
  TGCCGAGCCCGGCTATCCCCCGGAGCCTGGGGATCCCAAGAGCGACGACAGTGACGTGGACGTCTGCTTC
  ACAGACGACCAGAGCGACCCCGAGGTTGGGGGCGGCGGGGAGCGCGACTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214016 representing NM_021620
  Red=Cloning site Green=Tags(s)
  
  MHGAARAPATSVSADCCIPAGLRLGPVPGTFKLGKYLSDRREPGPKKKVLTIQTSAHQVRMVRGELVDES
  GGSPLEWIGLIRAARNSQEQTLEAIADLPGGQIFYRALRDVQPGEELTVWYSNSLAQWFDIPTTATPTHD
  EKGEERYICWYCWRTFRYPNSLKAHLRFHCVFSGGGGGAFLHHEHAARQGAVPAADGLGLSPKPPAPDFA
  APSQAGTLRPHPLGPPPVQACGAREGIKREASSAPSATSPTPGKWGQPKKGKEQLDRALDMSGAARGQGH
  FLGIVGGSSAGVGSLAFYPGVRSAFKPAGLARAAAAAHGDPYREESSSKQGAGLALGRLLGGGRACGRPG
  SGENSAAGGAGHHHHHHAHHHHHPKCLLAGDPPPPPPPGLPCSGALRGFPLLSVPPEEASAFKHVERAPP
  AAAALPGARYAQLPPAPGLPLERCALPPLDPGGLKAYPGGECSHLPAVMPAFTVYNGELLYGSPATTAYY
  PLKLHFGGLLKYPESISYFSGPAAAALSPAELGSLASIDREIAMHNQQLSEMAAGKGRGRLDSGTLPPAV
  AAAGGTGGGGSGGSGAGKPKTGHLCLYCGKLYSRKYGLKIHMRTHTGYKPLKCKVCLRPFGDPSNLNKHI
  RLHAEGNTPYRCEFCGKVLVRRRDLERHVKSRHPGQSLLAKAGDGPGAEPGYPPEPGDPKSDDSDVDVCF
  TDDQSDPEVGGGGERDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021620
• ORF Size: 3201 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021620.2, NP_067633.1
• RefSeq Size: 3234 bp
• RefSeq ORF: 2124 bp
• Locus ID: 59336
• UniProt ID: Q9H4Q3
• Cytogenetics: 6q16.2
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]