TEM4 (ARHGEF17) (NM_014786) Human Tagged ORF Clone

CAT#: RC214003

ARHGEF17 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 17 (ARHGEF17). Note: ORF is codon optimized

Product Images

Circular map for RC214003

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TEM4
• Synonyms: p164-RhoGEF; P164RHOGEF; RHOGEF17; TEM4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214003 ORF sequence, codon optimized.
  Due to the complexity of NM_014786, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGATGGCGCACCGCGCCCCCAGCTTTACAGATCCGTATCATTCAAGCTCCTGGAAAGGTGGTCTG
  GGGGACCAGGGCTGAGGGAGGAGGACACCGATACACCTGGTTTGCGCCGACGCGCTTCCTGCAGGCCTAC
  CACGGCTGCCCGGGGGCAGCCCAGTAGAAGGGTGTCAAAGCTTGCGAGCGGTCCACTCGCTGCTCCCGCC
  CAACCCCGACCCCTGAGGAGCCTGTCACCAAGTGTGCGGCAGTTGTCCCGCCGGTTTGACGCTCCCAGGC
  TCGATGACGGATCCGCTGGGACCAGGGACGGGGGTGTACTGCCCGCGGCTGCCGAGGAGGCCGCAGAAGG
  CCCGGCCCGCGGAGCCTGGCCGAGCGTCACCGAAATGCGGAAGCTGTTTGGGGGCCCAGGATCTAGAAGG
  CCAAGTGCCGACAGCGAGAGCCCCGGCACACCCAGCCCAGACGGGGCAGCCTGGGAACCCCCAGCTCGAG
  AGTCCCGCCAACCACCAACTCCTCCACCTCGCACCTGCTTCCCACTGGCTGGCCTGCGATCCGCTCGCCC
  CCTGACTGGGCCCGAAACTGAAGGAAGGCTCCGGCGCCCACAGCAGCAGCAGGAAAGAGCCCAGAGGCCG
  GCAGACGGACTCCATTCCTGGCACATTTTCAGCCAGCCGCAGGCAGGCGCGAGAGCATCATGTAGCTCCT
  CCAGCATTGCTGCCTCATACCCAGTATCTCGATCCAGGGCCGCCTCTAGCTCAGAGGAGGAAGAAGAAGG
  ACCACCCCAGTTGCCAGGGGCCCAGAGTCCAGCTTATCACGGAGGCCACAGCTCCGGGTCAGACGATGAC
  AGAGACGGGGAAGGTGGCCACCGCTGGGGCGGTCGACCGGGCCTCCGGCCCGGATCCAGCCTCCTGGACC
  AAGACTGCAGGCCCGACAGCGACGGCCTTAACCTCAGCTCAATGAACAGCGCCGGCGTTAGTGGGAGCCC
  TGAACCTCCCACCAGTCCTCGGGCACCTCGCGAGGAGGGCCTGCGGGAATGGGGATCTGGATCTCCTCCT
  TGTGTTCCAGGGCCCCAGGAAGGACTCCGCCCAATGTCCGACAGTGTGGGGGGGGCCTTTAGGGTTGCTA
  AAGTTAGCTTTCCCTCTTACTTGGCCTCACCCGCTGGCAGTCGCGGGTCTAGCAGATACAGTAGCACTGA
  AACCCTGAAGGACGATGACCTTTGGAGTTCCCGCGGTTCAGGGGGATGGGGAGTGTATAGAAGTCCATCT
  TTCGGAGCCGGTGAGGGGCTCCTGAGGAGTCAGGCCCGCACCAGGGCAAAGGGGCCTGGGGGTACATCCC
  GGGCTCTCCGGGACGGGGGCTTCGAACCGGAAAAGAGCAGACAACGGAAGTCCCTGTCCAACCCAGACAT
  TGCAAGCGAGACATTGACACTGCTGTCTTTCCTGCGATCTGATCTCAGCGAACTGCGCGTAAGGAAACCC
  GGAGGCAGTTCAGGGGACCGGGGCTCCAACCCACTTGATGGGAGAGACAGTCCCTCTGCAGGCGGTCCGG
  TGGGTCAATTGGAGCCTATCCCGATTCCCGCCCCCGCCTCCCCAGGCACTCGACCAACGCTCAAGGACCT
  GACTGCCACTCTGCGACGCGCAAAAAGTTTCACTTGCAGTGAAAAGCCAATGGCAAGAAGGCTTCCTCGA
  ACGTCCGCGCTGAAAAGCTCTTCTTCCGAGCTGCTGCTGACCGGTCCGGGGGCAGAGGAGGATCCCTTGC
  CTCTGATTGTTCAGGATCAATATGTCCAAGAGGCCCGGCAAGTGTTCGAGAAGATTCAGCGCATGGGCGC
  CCAGCAAGACGATGGCTCTGATGCCCCTCCAGGTTCTCCAGATTGGGCTGGGGATGTGACAAGGGGGCAG
  AGGAGCCAGGAGGAGCTGTCTGGCCCTGAATCTAGCCTGACTGACGAGGGCATAGGCGCAGATCCAGAGC
  CACCCGTCGCGGCATTTTGCGGACTGGGGACCACAGGCATGTGGCGGCCATTGTCTTCCTCCAGCGCCCA
  GACAAACCACCATGGACCCGGAACAGAAGATAGTCTGGGCGGCTGGGCACTGGTGTCCCCCGAGACGCCG
  CCTACCCCAGGTGCCCTGCGCCGGCGGCGGAAGGTGCCGCCAAGCGGATCTGGAGGGAGCGAACTTTCCA
  ATGGCGAAGCAGGCGAAGCGTATCGGTCCCTTTCCGATCCAATACCACAACGCCACCGCGCCGCCACCAG
  CGAAGAACCCACAGGCTTCTCCGTTGATTCCAACCTTCTCGGCAGCCTCTCACCCAAGACAGGATTGCCT
  GCGACGAGCGCCATGGACGAAGGGCTCACATCAGGACACTCTGACTGGTCCGTGGGCTCAGAGGAATCCA
  AGGGGTACCAGGAAGTAATTCAGAGTATCGTCCAGGGCCCGGGGACTTTGGGCCGAGTCGTTGACGACAG
  AATCGCAGGGAAAGCACCCAAAAAGAAATCCCTGTCTGACCCTTCAAGGAGGGGGGAGCTGGCCGGGCCT
  GGGTTCGAGGGGCCAGGTGGCGAACCGATCCGAGAGGTGGAGCCCATGCTCCCGCCCTCCTCCAGTGAGC
  CAATCCTGGTGGAACAGAGGGCTGAGCCAGAAGAACCGGGCGCCACCCGCAGCCGAGCTCAGAGTGAGCG
  CGCATTGCCGGAAGCACTGCCTCCTCCCGCCACTGCCCATAGGAACTTTCATCTGGACCCTAAGCTGGCC
  GACATCCTGAGTCCAAGGTTGATCAGGCGGGGTAGCAAAAAGCGCCCCGCTCGAAGTAGTCACCAGGAGC
  TGAGGCGAGACGAAGGGAGCCAGGATCAAACAGGCAGCCTTTCCCGGGCCCGACCCAGCTCTCGGCACGT
  GAGGCACGCCTCCGTGCCCGCCACCTTCATGCCTATTGTGGTTCCCGAGCCTCCTACTAGTGTGGGGCCA
  CCAGTGGCTGTGCCTGAGCCAATAGGCTTCCCCACCAGGGCGCACCCCACGCTGCAGGCCCCCTCTCTTG
  AGGACGTGACCAAACAGTATATGTTGAACCTGCACTCCGGGGAGGTCCCAGCCCCTGTACCCGTCGATAT
  GCCATGCCTGCCTCTGGCCGCCCCACCTTCCGCCGAAGCGAAACCTCCCGAGGCCGCCCGCCCCGCCGAT
  GAACCGACCCCAGCTTCAAAGTGCTGTAGTAAACCCCAGGTGGATATGAGGAAACACGTTGCCATGACAC
  TGCTCGACACGGAGCAGAGTTATGTGGAATCTCTCCGAACACTGATGCAGGGGTATATGCAACCTTTGAA
  ACAGCCTGAAAATAGTGTGCTGTGCGACCCTTCACTGGTTGACGAGATCTTTGACCAGATTCCCGAGTTG
  CTTGAACACCACGAACAATTTCTCGAACAAGTTAGACATTGCATGCAGACATGGCACGCACAACAGAAAG
  TTGGTGCTCTGCTCGTGCAGTCTTTCAGCAAGGATGTGCTGGTTAACATCTATTCAGCTTACATTGACAA
  TTTTCTGAATGCCAAGGATGCGGTACGGGTTGCTAAGGAAGCCCGACCCGCCTTCCTCAAGTTTCTGGAG
  CAATCAATGCGCGAGAACAAAGAAAAGCAGGCACTCTCCGACCTTATGATCAAACCCGTACAACGAATCC
  CCAGGTATGAGCTGCTCGTGAAGGACTTGTTGAAGCACACCCCTGAAGATCATCCTGACCACCCTCTTCT
  GTTGGAGGCCCAGAGAAACATTAAACAGGTCGCCGAGCGAATCAATAAAGGGGTGCGCTCTGCCGAAGAG
  GCTGAGCGCCATGCGAGGGTGCTGCAGGAAATCGAGGCGCATATCGAAGGAATGGAGGACCTGCAAGCCC
  CCCTGAGAAGATTCCTCCGCCAGGAAATGGTGATTGAAGTGAAAGCGATCGGGGGTAAAAAGGATCGATC
  CCTGTTTTTGTTCACAGACTTGATCGTTTGCACTACACTCAAGCGGAAGAGCGGCTCTCTGCGGAGATCT
  AGTATGAGTCTTTACACAGCTGCCAGTGTCATAGATACAGCATCCAAGTATAAGATGCTGTGGAAACTGC
  CACTCGAGGATGCAGACATTATCAAGGGTGCCTCTCAGGCGACCAACCGGGAAAATATTCAAAAGGCAAT
  TTCCCGCCTGGACGAGGATCTTACAACCCTGGGGCAGATGAGCAAACTGTCAGAGAGCCTGGGGTTTCCT
  CACCAGTCACTGGACGACGCCCTGCGCGATTTGAGCGCCGCCATGCACAGGGACTTGAGCGAGAAACAGG
  CCCTCTGCTACGCTTTGTCTTTCCCTCCTACAAAACTGGAGCTCTGCGCCACCAGACCCGAAGGTACCGA
  CAGCTATATCTTCGAATTTCCTCATCCCGACGCCAGGTTGGGGTTTGAACAGGCTTTTGACGAAGCTAAG
  CGGAAACTGGCAAGCAGTAAGTCATGCCTGGACCCCGAGTTTCTTAAGGCCATTCCGATAATGAAGACAA
  GGTCAGGCATGCAGTTCAGTTGTGCTGCGCCTACCCTGAACTCCTGTCCTGAGCCCAGCCCCGAGGTGTG
  GGTTTGCAACTCTGATGGTTACGTTGGGCAGGTTTGCCTGCTGAGTCTGAGGGCAGAACCGGACGTGGAA
  GCATGCATAGCCGTGTGCTCCGCACGCATTCTGTGCATCGGGGCGGTCCCTGGCCTGCAACCAAGGTGTC
  ACCGGGAACCTCCTCCCTCTCTGAGAAGTCCGCCCGAGACTGCCCCAGAGCCAGCCGGCCCCGAACTGGA
  CGTTGAGGCTGCCGCAGACGAAGAAGCTGCCACACTCGCCGAGCCTGGTCCCCAGCCCTGCCTGCACATC
  TCAATAGCGGGCTCCGGCCTTGAGATGACCCCGGGCCTCGGCGAGGGCGATCCTCGACCCGAACTCGTGC
  CTTTCGACTCTGACAGCGACGACGAATCCTCACCCTCTCCCAGCGGTACGCTGCAGAGTCAGGCCAGTAG
  AAGCACAATATCTTCCTCATTTGGGAATGAGGAAACACCTAGTAGCAAGGAGGCAACAGCTGAAACCACA
  AGCAGTGAAGAGGAACAGGAGCCTGGTTTTTTGCCTTTGTCCGGGTCATTCGGCCCCGGTGGCCCCTGTG
  GAACGTCACCGATGGATGGAAGAGCCCTGAGAAGATCCTCACATGGTTCCTTCACGCGAGGCAGCCTGGA
  GGACCTCCTCTCAGTGGACCCCGAGGCATATCAGTCATCAGTGTGGTTGGGAACCGAGGATGGCTGTGTT
  CACGTCTATCAAAGCTCCGATTCTATCCGGGATAGGAGGAATTCAATGAAACTGCAGCACGCTGCATCTG
  TCACTTGCATCTTGTACCTGAACAACCAGGTGTTCGTCTCCCTTGCAAATGGTGAGCTGGTTGTGTATCA
  AAGGGAGGCCGGTCACTTCTGGGACCCTCAGAATTTCAAATCAGTCACCCTGGGTACGCAAGGCTCCCCT
  ATAACTAAGATGGTGTCCGTCGGAGGCAGACTTTGGTGCGGATGCCAGAATCGCGTATTGGTCTTGAGCC
  CGGACACCCTCCAGCTGGAACATATGTTCTACGTGGGGCAGGACTCTTCACGCTGCGTAGCCTGTATGGT
  CGATTCCAGTCTGGGCGTTTGGGTGACTCTCAAAGGATCCGCACACGTTTGTCTGTATCACCCGGATACA
  TTTGAACAGCTGGCTGAGGTGGACGTTACTCCTCCTGTGCACCGGATGCTGGCCGGTAGCGACGCAATTA
  TCCGGCAACACAAGGCAGCTTGTTTGAGGATCACCGCCCTGCTCGTGTGTGAGGAGTTGCTTTGGGTGGG
  GACAAGCGCTGGAGTGGTGCTGACAATGCCGACTTCTCCTGGCACGGTCAGCTGCCCCAGGGCCCCACTG
  TCTCCAACTGGACTCGGCCAGGGACACACTGGGCACGTTAGGTTCCTCGCTGCCGTTCAGCTGCCAGATG
  GCTTCAATCTTCTGTGTCCTACACCACCTCCCCCTCCTGACACGGGCCCCGAAAAACTGCCCTCCCTCGA
  GCACAGGGACTCTCCCTGGCATCGCGGGCCGGCCCCTGCCAGACCCAAAATGCTGGTGATTTCCGGGGGA
  GACGGTTATGAAGACTTTCGGCTCTCATCAGGGGGCGGCTCCTCTTCTGAGACAGTGGGCCGGGATGACT
  CCACTAATCACTTGCTTCTCTGGCGGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214003 representing NM_014786
  Red=Cloning site Green=Tags(s)
  
  MADGAPRPQLYRSVSFKLLERWSGGPGLREEDTDTPGLRRRASCRPTTAARGQPSRRVSKLASGPLAAPA
  QPRPLRSLSPSVRQLSRRFDAPRLDDGSAGTRDGGVLPAAAEEAAEGPARGAWPSVTEMRKLFGGPGSRR
  PSADSESPGTPSPDGAAWEPPARESRQPPTPPPRTCFPLAGLRSARPLTGPETEGRLRRPQQQQERAQRP
  ADGLHSWHIFSQPQAGARASCSSSSIAASYPVSRSRAASSSEEEEEGPPQLPGAQSPAYHGGHSSGSDDD
  RDGEGGHRWGGRPGLRPGSSLLDQDCRPDSDGLNLSSMNSAGVSGSPEPPTSPRAPREEGLREWGSGSPP
  CVPGPQEGLRPMSDSVGGAFRVAKVSFPSYLASPAGSRGSSRYSSTETLKDDDLWSSRGSGGWGVYRSPS
  FGAGEGLLRSQARTRAKGPGGTSRALRDGGFEPEKSRQRKSLSNPDIASETLTLLSFLRSDLSELRVRKP
  GGSSGDRGSNPLDGRDSPSAGGPVGQLEPIPIPAPASPGTRPTLKDLTATLRRAKSFTCSEKPMARRLPR
  TSALKSSSSELLLTGPGAEEDPLPLIVQDQYVQEARQVFEKIQRMGAQQDDGSDAPPGSPDWAGDVTRGQ
  RSQEELSGPESSLTDEGIGADPEPPVAAFCGLGTTGMWRPLSSSSAQTNHHGPGTEDSLGGWALVSPETP
  PTPGALRRRRKVPPSGSGGSELSNGEAGEAYRSLSDPIPQRHRAATSEEPTGFSVDSNLLGSLSPKTGLP
  ATSAMDEGLTSGHSDWSVGSEESKGYQEVIQSIVQGPGTLGRVVDDRIAGKAPKKKSLSDPSRRGELAGP
  GFEGPGGEPIREVEPMLPPSSSEPILVEQRAEPEEPGATRSRAQSERALPEALPPPATAHRNFHLDPKLA
  DILSPRLIRRGSKKRPARSSHQELRRDEGSQDQTGSLSRARPSSRHVRHASVPATFMPIVVPEPPTSVGP
  PVAVPEPIGFPTRAHPTLQAPSLEDVTKQYMLNLHSGEVPAPVPVDMPCLPLAAPPSAEAKPPEAARPAD
  EPTPASKCCSKPQVDMRKHVAMTLLDTEQSYVESLRTLMQGYMQPLKQPENSVLCDPSLVDEIFDQIPEL
  LEHHEQFLEQVRHCMQTWHAQQKVGALLVQSFSKDVLVNIYSAYIDNFLNAKDAVRVAKEARPAFLKFLE
  QSMRENKEKQALSDLMIKPVQRIPRYELLVKDLLKHTPEDHPDHPLLLEAQRNIKQVAERINKGVRSAEE
  AERHARVLQEIEAHIEGMEDLQAPLRRFLRQEMVIEVKAIGGKKDRSLFLFTDLIVCTTLKRKSGSLRRS
  SMSLYTAASVIDTASKYKMLWKLPLEDADIIKGASQATNRENIQKAISRLDEDLTTLGQMSKLSESLGFP
  HQSLDDALRDLSAAMHRDLSEKQALCYALSFPPTKLELCATRPEGTDSYIFEFPHPDARLGFEQAFDEAK
  RKLASSKSCLDPEFLKAIPIMKTRSGMQFSCAAPTLNSCPEPSPEVWVCNSDGYVGQVCLLSLRAEPDVE
  ACIAVCSARILCIGAVPGLQPRCHREPPPSLRSPPETAPEPAGPELDVEAAADEEAATLAEPGPQPCLHI
  SIAGSGLEMTPGLGEGDPRPELVPFDSDSDDESSPSPSGTLQSQASRSTISSSFGNEETPSSKEATAETT
  SSEEEQEPGFLPLSGSFGPGGPCGTSPMDGRALRRSSHGSFTRGSLEDLLSVDPEAYQSSVWLGTEDGCV
  HVYQSSDSIRDRRNSMKLQHAASVTCILYLNNQVFVSLANGELVVYQREAGHFWDPQNFKSVTLGTQGSP
  ITKMVSVGGRLWCGCQNRVLVLSPDTLQLEHMFYVGQDSSRCVACMVDSSLGVWVTLKGSAHVCLYHPDT
  FEQLAEVDVTPPVHRMLAGSDAIIRQHKAACLRITALLVCEELLWVGTSAGVVLTMPTSPGTVSCPRAPL
  SPTGLGQGHTGHVRFLAAVQLPDGFNLLCPTPPPPPDTGPEKLPSLEHRDSPWHRGPAPARPKMLVISGG
  DGYEDFRLSSGGGSSSETVGRDDSTNHLLLWRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014786
• ORF Size: 6189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014786.2, NM_014786.3, NP_055601.2
• RefSeq Size: 7830 bp
• RefSeq ORF: 6192 bp
• Locus ID: 9828
• UniProt ID: Q96PE2
• Cytogenetics: 11q13.4
• Protein Families: Druggable Genome
• MW: 221.7 kDa
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases.[UniProtKB/Swiss-Prot Function]