PTPN18 (NM_014369) Human Tagged ORF Clone

CAT#: RC213476

PTPN18 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 18 (brain-derived) (PTPN18), transcript variant 1

Product Images

Circular map for RC213476

• 

  Coomassie blue staining of purified PTPN18 protein (Cat# TP313476). The protein was produced from HEK293T cells transfected with PTPN18 cDNA clone (Cat# RC213476) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPN18
• Synonyms: BDP1; PTP-HSCF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213476 representing NM_014369
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGCAGCCTGGACTCGGCGCGGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGG
  CAGTCCTCGCCGGCGAGTTCAGCGACATCCAGGCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGTGCTC
  CACCGTGGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGCTGCCTTATGATCAG
  ACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACTACATTAATGGCAACTTCATCCGGG
  GCGTGGATGGAAGCCTGGCCTACATTGCCACGCAAGGACCCTTGCCTCACACCCTGCTAGACTTCTGGAG
  ACTGGTCTGGGAGTTTGGGGTCAAGGTGATCCTGATGGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGG
  TGTGAGCGGTACTGGGCCCAGGAGCAGGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGG
  AGAAGTGGCTGAATGAGGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGT
  GTACCAGCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCGCCATG
  GTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACTGCAGTGCGGGTTGTG
  GGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGCTCCTGACCCAGATGATCCCACCTGA
  CTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGAAGCAGCGGCCTGCGGCCGTGCAGACAGAGGAGCAG
  TACAGGTTCCTGTACCACACGGTGGCTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCCCCCACTACC
  AGAACATCAAAGAGAATTGTGCCCCACTCTACGACGATGCCCTCTTCCTCCGGACTCCCCAGGCACTTCT
  CGCCATACCCCGCCCACCAGGAGGGGTCCTCAGGAGCATCTCTGTGCCCGGGTCCCCGGGCCACGCCATG
  GCTGACACCTACGCGGTGGTGCAGAAGCGCGGGGCTCCAGCGGGCGCCGGGAGTGGGACGCAGACGGGGA
  CGGGGACGGGGACGGGGGCGCGCAGCGCGGAGGAGGCGCCGCTCTACAGCAAGGTGACGCCGCGCGCCCA
  GCGACCCGGGGCGCACGCGGAGGACGCGAGGGGGACGCTGCCTGGCCGCGTTCCTGCTGACCAAAGTCCT
  GCCGGATCTGGCGCCTACGAGGACGTGGCGGGTGGAGCTCAGACCGGTGGGCTAGGTTTCAACCTGCGCA
  TTGGGAGGCCGAAGGGTCCCCGGGACCCGCCTGCTGAGTGGACCCGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213476 representing NM_014369
  Red=Cloning site Green=Tags(s)
  
  MSRSLDSARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNRYKDVLPYDQ
  TRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWEFGVKVILMACREIENGRKR
  CERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPSSPDHMLAM
  VEEARRLQGSGPEPLCVHCSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQ
  YRFLYHTVAQMFCSTLQNASPHYQNIKENCAPLYDDALFLRTPQALLAIPRPPGGVLRSISVPGSPGHAM
  ADTYAVVQKRGAPAGAGSGTQTGTGTGTGARSAEEAPLYSKVTPRAQRPGAHAEDARGTLPGRVPADQSP
  AGSGAYEDVAGGAQTGGLGFNLRIGRPKGPRDPPAEWTRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014369
• ORF Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014369.4
• RefSeq Size: 2837 bp
• RefSeq ORF: 1383 bp
• Locus ID: 26469
• UniProt ID: Q99952
• Cytogenetics: 2q21.1
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• MW: 50.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. This PTP contains a PEST motif, which often serves as a protein-protein interaction domain, and may be related to protein intracellular half-live. This protein can differentially dephosphorylate autophosphorylated tyrosine kinases that are overexpressed in tumor tissues, and it appears to regulate HER2, a member of the epidermal growth factor receptor family of receptor tyrosine kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]