PTPN18 (NM_014369) Human Untagged Clone

CAT#: SC102683

PTPN18 (untagged)-Human protein tyrosine phosphatase, non-receptor type 18 (brain-derived) (PTPN18), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPN18
• Synonyms: BDP1; PTP-HSCF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014369 edited
  GGGCGGCCGCGAATTCGGCACGAGGGAGGCAAGGGCGGGGACTCAGTCACCGGCCTCCGC
  CTCCCGCGGCGTCCACACTCGCCGCGCGCGCGGCGGCCGGGCTGGACCTTGCTGGCCCGC
  GGCGCCATGAGCCGCAGCCTGGACTCGGCGCGGAGCTTCCTGGAGCGGCTGGAAGCGCGG
  GGCGGCCGGGAGGGGGCAGTCCTCGCCGGCGAGTTCAGCGACATCCAGGCCTGCTCGGCC
  GCCTGGAAGGCTGACGGCGTGTGCTCCACCGTGGCCGGCAGTCGGCCAGAGAACGTGAGG
  AAGAACCGCTACAAAGACGTGCTGCCTTATGATCAGACGCGAGTAATCCTCTCCCTGCTC
  CAGGAAGAGGGACACAGCGACTACATTAATGGCAACTTCATCCGGGGCGTGGATGGAAGC
  CTGGCCTACATTGCCACGCAAGGACCCTTGCCTCACACCCTGCTAGACTTCTGGAGACTG
  GTCTGGGAGTTTGGGGTCAAGGTGATCCTGATGGCCTGTCGAGAGATAGAGAATGGGCGG
  AAAAGGTGTGAGCGGTACTGGGCCCAGGAGCAGGAGCCACTGCAGACTGGGCTTTTCTGC
  ATCACTCTGATAAAGGAGAAGTGGCTGAATGAGGACATCATGCTCAGGACCCTCAAGGTC
  ACATTCCAGAAGGAGTCCCGTTCTGTGTACCAGCTACAGTATATGTCCTGGCCAGACCGT
  GGGGTCCCCAGCAGTCCTGACCACATGCTCGCCATGGTGGAGGAAGCCCGTCGCCTCCAG
  GGATCTGGCCCTGAACCCCTCTGTGTCCACTGCAGTGCGGGTTGTGGGCGAACAGGCGTC
  CTGTGCACCGTGGATTATGTGAGGCAGCTGCTCCTGACCCAGATGATCCCACCTGACTTC
  AGTCTCTTTGATGTGGTCCTTAAGATGAGGAAGCAGCGGCCTGCGGCCGTGCAGACAGAG
  GAGCAGTACAGGTTCCTGTACCACACGGTGGCTCAGATGTTCTGCTCCACACTCCAGAAT
  GCCAGCCCCCACTACCAGAACATCAAAGAGAATTGTGCCCCACTCTACGACGATGCCCTC
  TTCCTCCGGACTCCCCAGGCACTTCTCGCCATACCCCGCCCACCAGGAGGGGTCCTCAGG
  AGCATCTCTGTGCCCGGGTCCCCGGGCCACGCCATGGCTGACACCTACGCGGTGGTGCAG
  AAGCGCGGGGCTCCAGCGGGCGCCGGGAGTGGGACGCAGACGGGGACGGGGACGGGGGCG
  CGCAGCGCGGAGGAGGCGCCGCTCTACAGCAAGGTGACGCCGCGCGCCCAGCGACCCGGG
  GCGCACGCGGAGGACGCGAGGGGGACGCTGCCTGGCCGCGTTCCTGCTGACCAAAGTCCT
  GCCGGATCTGGCGCCTACGAGGACGTGGCGGGTGGAGCTCAGACCGGTGGGCTAGGTTTC
  AACCTGCGCATTGGGAGGCCGAAGGGTCCCCGGGACCCGCCTGCTGAGTGGACCCGGGTG
  TAAGTCTAACGCCAGTTCCTGCCTGTTGCCTCTTGTGAGCTCGGACTGCTGATGCCCCGG
  TGCTGCTGAGCGCCGTGCGCAGAATGGAAACAGTGGGCCTGGATCAAAGTTAAAGTTTCT
  CAGGGTGGGAAATGTGGGAGGCTTTGCCCCAATGACTGTAGCATTCAAGGCTTGAGGCTG
  GAGGAGGTAGCTAGGGTATAGTGGCTGGTGAGGCTGCACAGAGCAGATTCAAGAAAGAAG
  ATCAGGAAGGGGCATGACCCCTGAGTTATG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014369 unedited
  AAAGAGTTTACAATAGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGAGGCA
  AGGCGGGGACTCAGTCACCGGCCTCCGCCTCCCGCGGCGTCCACACTCGCCGCGCGCGCG
  GCGGCCGGGCTGGACCTTGCTGGCCCGCGGCGCCATGAGCCGCAGCCTGGACTCGGCGCG
  GAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCGA
  GTTCAGCGACATCCAGGCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGTGCTCCACCGT
  GGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGCTGCCTTATGA
  TCAGACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACTACATTAATGG
  CAACTTCATCCGGGGCGTGGATGGAAGCCTGGCCTACATTGCCACGCAAGGACCCTTGCC
  TCACACCCTGCTAGACTTCTGGAGACTGGTCTGGGAGTTTGGGGTCAAGGTGATCCTGAT
  GGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGGTGTGAGCGGTACTGGGCCCAGGAGCA
  GGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATGA
  GGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACCA
  GCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCGC
  CATGGGTGGAGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACTG
  CAGTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGNATATGTGNAGCAGCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014369 unedited
  TGGCGTGTGCCTCCTCTTTTTTCTTTTAACAGCCGCTTCAAGTTTTATCCTAGCAGTAAC
  CCTACCATGGGCCATTTAAAGTGTTTCAAATCTTATGGGGCTGAGCCTGGTGGCTCACAC
  CTGTAATCTCAGCACTTTGGGAGGCTGAGGCAAGACGATTGCTCGACGTTGGCAGTTCAA
  GACCAGTCTGGGCAACATGGCAAAAACTCATTACTTAAAAAAAAAAAAAATTACACCTGT
  AGTCCCACCTCTTCCGCAGGCTGCGTGAACAGCAAGGGGTCTATCTGGTTGGGATTCTGA
  CTGGCGTAGGTTTGCCTGGACTCTATCTCATTAGGTACCTGGTTCGTCAGGGGACCTTCC
  AGTCTGTATAATTGTTTCTTTCCCCTTATTCTTCTTTTCTTTGTCCTCTCCCTCCTCACC
  CCCCTCCCCCTTCCCTTTTGTTCCTTATTCCTTCCCCTCCTTTCCCTCTTCGCTTCCCAT
  CTCCTCTCACTTTTTCATACTCCCCCCCCCTACCATCCCTGTTTTCTTTTCTCCCCCCTC
  CTTCCCCCCCTCCCTATCTTTTATCATCCCTCACTTTCCTTTTCCCAATTTCACCTTTGT
  TCTACGCCCTTTTCTCTTCCCTCCCTTCCTCTTACTCTTCTCTTTTCCCTTTTTCTCTTC
  TCCCATCCTTTTTACGCTCCCACCTCCCTCTTTTCTCCCCTTTTCTCACCCTCCCATCAC
  CCTTTCTATTACATCCCCCCCACCCTCTTCCTTTCCCTTCCTCCTCCCCCGCACTCCCTA
  TCTACCTCTCTACTCCTCCCCATCTTCTCTTATCATATTCCCATTTTTCTCTAACTTTTC
  CCCCCTTCACTTTTCTCTCCCCATCTCCCATCGCTTTTTATCCCATATCCGTCACCAATA
  CTCCCTCCACCTCCCCTCTGTTTATATCGCTCCCACCTTTCCCACATTTCATATAACTCA
  ATTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014369
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014369.2, NP_055184.2
• RefSeq Size: 2837 bp
• RefSeq ORF: 1383 bp
• Locus ID: 26469
• UniProt ID: Q99952
• Cytogenetics: 2q21.1
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. This PTP contains a PEST motif, which often serves as a protein-protein interaction domain, and may be related to protein intracellular half-live. This protein can differentially dephosphorylate autophosphorylated tyrosine kinases that are overexpressed in tumor tissues, and it appears to regulate HER2, a member of the epidermal growth factor receptor family of receptor tyrosine kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).