CNGB3 (NM_019098) Human Tagged ORF Clone

CAT#: RC212989

CNGB3 (Myc-DDK-tagged)-Human cyclic nucleotide gated channel beta 3 (CNGB3)

Product Images

Circular map for RC212989

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNGB3
• Synonyms: ACHM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212989 representing NM_019098.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTAAATCGCTGACAAAAGTCAACAAGGTGAAGCCTATAGGAGAGAACAATGAGAATGAACAAAGT
  TCTCGTCGGAATGAAGAAGGCTCTCACCCAAGTAATCAGTCTCAGCAAACCACAGCACAGGAAGAAAAC
  AAAGGTGAAGAGAAATCTCTCAAAACCAAGTCAACTCCAGTCACGTCTGAAGAGCCACACACCAACATA
  CAAGACAAACTCTCCAAGAAAAATTCCTCTGGAGATCTGACCACAAACCCTGACCCTCAAAATGCAGCA
  GAACCAACTGGAACAGTGCCAGAGCAGAAGGAAATGGACCCCGGGAAAGAAGGTCCAAACAGCCCACAA
  AACAAACCGCCTGCAGCTCCTGTTATAAATGAGTATGCCGATGCCCAGCTACACAACCTGGTGAAAAGA
  ATGCGTCAAAGAACAGCCCTCTACAAGAAAAAGTTGGTAGAGGGAGATCTCTCCTCACCCGAAGCCAGC
  CCACAAACTGCAAAGCCCACGGCTGTACCACCAGTAAAAGAAAGCGATGATAAGCCAACAGAACATTAC
  TACAGGCTGTTGTGGTTCAAAGTCAAAAAGATGCCTTTAACAGAGTACTTAAAGCGAATTAAACTTCCA
  AACAGCATAGATTCATACACAGATCGACTCTATCTCCTGTGGCTCTTGCTTGTCACTCTTGCCTATAAC
  TGGAACTGCTGTTTTATACCACTGCGCCTCGTCTTCCCATATCAAACCGCAGACAACATACACTACTGG
  CTTATTGCGGACATCATATGTGATATCATCTACCTTTATGATATGCTATTTATCCAGCCCAGACTCCAG
  TTTGTAAGAGGAGGAGACATAATAGTGGATTCAAATGAGCTAAGGAAACACTACAGGACTTCTACAAAA
  TTTCAGTTGGATGTCGCATCAATAATACCATTTGATATTTGCTACCTCTTCTTTGGGTTTAATCCAATG
  TTTAGAGCAAATAGGATGTTAAAGTACACTTCATTTTTTGAATTTAATCATCACCTAGAGTCTATAATG
  GACAAAGCATATATCTACAGAGTTATTCGAACAACTGGATACTTGCTGTTTATTCTGCACATTAATGCC
  TGTGTTTATTACTGGGCTTCAAACTATGAAGGAATTGGCACTACTAGATGGGTGTATGATGGGGAAGGA
  AACGAGTATCTGAGATGTTATTATTGGGCAGTTCGAACTTTAATTACCATTGGTGGCCTTCCAGAACCA
  CAAACTTTATTTGAAATTGTTTTTCAACTCTTGAATTTTTTTTCTGGAGTTTTTGTGTTCTCCAGTTTA
  ATTGGTCAGATGAGAGATGTGATTGGAGCAGCTACAGCCAATCAGAACTACTTCCGCGCCTGCATGGAT
  GACACCATTGCCTACATGAACAATTACTCCATTCCTAAACTTGTGCAAAAGCGAGTTCGGACTTGGTAT
  GAATATACATGGGACTCTCAAAGAATGCTAGATGAGTCTGATTTGCTTAAGACCCTACCAACTACGGTC
  CAGTTAGCCCTCGCCATTGATGTGAACTTCAGCATCATCAGCAAAGTCGACTTGTTCAAGGGTTGTGAT
  ACACAGATGATTTATGACATGTTGCTAAGATTGAAATCCGTTCTCTATTTGCCTGGTGACTTTGTCTGC
  AAAAAGGGAGAAATTGGCAAGGAAATGTATATCATCAAGCATGGAGAAGTCCAAGTTCTTGGAGGCCCT
  GATGGTACTAAAGTTCTGGTTACTCTGAAAGCTGGGTCGGTGTTTGGAGAAATCAGCCTTCTAGCAGCA
  GGAGGAGGAAACCGTCGAACTGCCAATGTGGTGGCCCACGGGTTTGCCAATCTTTTAACTCTAGACAAA
  AAGACCCTCCAAGAAATTCTAGTGCATTATCCAGATTCTGAAAGGATCCTCATGAAGAAAGCCAGAGTG
  CTTTTAAAGCAGAAGGCTAAGACCGCAGAAGCAACCCCTCCAAGAAAAGATCTTGCCCTCCTCTTCCCA
  CCGAAAGAAGAGACACCCAAACTGTTTAAAACTCTCCTAGGAGGCACAGGAAAAGCAAGTCTTGCAAGA
  CTACTCAAATTGAAGCGAGAGCAAGCAGCTCAGAAGAAAGAAAATTCTGAAGGAGGAGAGGAAGAAGGA
  AAAGAAAATGAAGATAAACAAAAAGAAAATGAAGATAAACAAAAAGAAAATGAAGATAAAGGAAAAGAA
  AATGAAGATAAAGATAAAGGAAGAGAGCCAGAAGAGAAGCCACTGGACAGACCTGAATGTACAGCAAGT
  CCTATTGCAGTGGAGGAAGAACCCCACTCAGTTAGAAGGACAGTTTTACCCAGAGGGACTTCTCGTCAA
  TCACTCATTATCAGCATGGCTCCTTCTGCTGAGGGCGGAGAAGAGGTTCTTACTATTGAAGTCAAAGAA
  AAGGCTAAGCAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC212989
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFKSLTKVNKVKPIGENNENEQSSRRNEEGSHPSNQSQQTTAQEENKGEEKSLKTKSTPVTSEEPHTNI
  QDKLSKKNSSGDLTTNPDPQNAAEPTGTVPEQKEMDPGKEGPNSPQNKPPAAPVINEYADAQLHNLVKR
  MRQRTALYKKKLVEGDLSSPEASPQTAKPTAVPPVKESDDKPTEHYYRLLWFKVKKMPLTEYLKRIKLP
  NSIDSYTDRLYLLWLLLVTLAYNWNCCFIPLRLVFPYQTADNIHYWLIADIICDIIYLYDMLFIQPRLQ
  FVRGGDIIVDSNELRKHYRTSTKFQLDVASIIPFDICYLFFGFNPMFRANRMLKYTSFFEFNHHLESIM
  DKAYIYRVIRTTGYLLFILHINACVYYWASNYEGIGTTRWVYDGEGNEYLRCYYWAVRTLITIGGLPEP
  QTLFEIVFQLLNFFSGVFVFSSLIGQMRDVIGAATANQNYFRACMDDTIAYMNNYSIPKLVQKRVRTWY
  EYTWDSQRMLDESDLLKTLPTTVQLALAIDVNFSIISKVDLFKGCDTQMIYDMLLRLKSVLYLPGDFVC
  KKGEIGKEMYIIKHGEVQVLGGPDGTKVLVTLKAGSVFGEISLLAAGGGNRRTANVVAHGFANLLTLDK
  KTLQEILVHYPDSERILMKKARVLLKQKAKTAEATPPRKDLALLFPPKEETPKLFKTLLGGTGKASLAR
  LLKLKREQAAQKKENSEGGEEEGKENEDKQKENEDKQKENEDKGKENEDKDKGREPEEKPLDRPECTAS
  PIAVEEEPHSVRRTVLPRGTSRQSLIISMAPSAEGGEEVLTIEVKEKAKQ
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019098
• ORF Size: 2427 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019098.5
• RefSeq Size: 4369 bp
• RefSeq ORF: 2430 bp
• Locus ID: 54714
• UniProt ID: Q9NQW8
• Cytogenetics: 8q21.3
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• MW: 92.2 kDa
• Gene Summary: This gene encodes the beta subunit of a cyclic nucleotide-gated ion channel. The encoded beta subunit appears to play a role in modulation of channel function in cone photoreceptors. This heterotetrameric channel is necessary for sensory transduction, and mutations in this gene have been associated with achromatopsia 3, progressive cone dystrophy, and juvenile macular degeneration, also known as Stargardt Disease. [provided by RefSeq, Feb 2010]