CNGB3 (NM_019098) Human Tagged ORF Clone

CAT#: RG212989

CNGB3 (tGFP-tagged) - Human cyclic nucleotide gated channel beta 3 (CNGB3)

Product Images

Circular map for RG212989

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNGB3
• Synonyms: ACHM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212989 representing NM_019098
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAAATCGCTGACAAAAGTCAACAAGGTGAAGCCTATAGGAGAGAACAATGAGAATGAACAAAGTT
  CTCGTCGGAATGAAGAAGGCTCTCACCCAAGTAATCAGTCTCAGCAAACCACAGCACAGGAAGAAAACAA
  AGGTGAAGAGAAATCTCTCAAAACCAAGTCAACTCCAGTCACGTCTGAAGAGCCACACACCAACATACAA
  GACAAACTCTCCAAGAAAAATTCCTCTGGAGATCTGACCACAAACCCTGACCCTCAAAATGCAGCAGAAC
  CAACTGGAACAGTGCCAGAGCAGAAGGAAATGGACCCCGGGAAAGAAGGTCCAAACAGCCCACAAAACAA
  ACCGCCTGCAGCTCCTGTTATAAATGAGTATGCCGATGCCCAGCTACACAACCTGGTGAAAAGAATGCGT
  CAAAGAACAGCCCTCTACAAGAAAAAGTTGGTAGAGGGAGATCTCTCCTCACCCGAAGCCAGCCCACAAA
  CTGCAAAGCCCACGGCTGTACCACCAGTAAAAGAAAGCGATGATAAGCCAACAGAACATTACTACAGGCT
  GTTGTGGTTCAAAGTCAAAAAGATGCCTTTAACAGAGTACTTAAAGCGAATTAAACTTCCAAACAGCATA
  GATTCATACACAGATCGACTCTATCTCCTGTGGCTCTTGCTTGTCACTCTTGCCTATAACTGGAACTGCT
  GGTTTATACCACTGCGCCTCGTCTTCCCATATCAAACCGCAGACAACATACACTACTGGCTTATTGCGGA
  CATCATATGTGATATCATCTACCTTTATGATATGCTATTTATCCAGCCCAGACTCCAGTTTGTAAGAGGA
  GGAGACATAATAGTGGATTCAAATGAGCTAAGGAAACACTACAGGACTTCTACAAAATTTCAGTTGGATG
  TCGCATCAATAATACCATTTGATATTTGCTACCTCTTCTTTGGGTTTAATCCAATGTTTAGAGCAAATAG
  GATGTTAAAGTACACTTCATTTTTTGAATTTAATCATCACCTAGAGTCTATAATGGACAAAGCATATATC
  TACAGAGTTATTCGAACAACTGGATACTTGCTGTTTATTCTGCACATTAATGCCTGTGTTTATTACTGGG
  CTTCAAACTATGAAGGAATTGGCACTACTAGATGGGTGTATGATGGGGAAGGAAACGAGTATCTGAGATG
  TTATTATTGGGCAGTTCGAACTTTAATTACCATTGGTGGCCTTCCAGAACCACAAACTTTATTTGAAATT
  GTTTTTCAACTCTTGAATTTTTTTTCTGGAGTTTTTGTGTTCTCCAGTTTAATTGGTCAGATGAGAGATG
  TGATTGGAGCAGCTACAGCCAATCAGAACTACTTCCGCGCCTGCATGGATGACACCATTGCCTACATGAA
  CAATTACTCCATTCCTAAACTTGTGCAAAAGCGAGTTCGGACTTGGTATGAATATACATGGGACTCTCAA
  AGAATGCTAGATGAGTCTGATTTGCTTAAGACCCTACCAACTACGGTCCAGTTAGCCCTCGCCATTGATG
  TGAACTTCAGCATCATCAGCAAAGTCGACTTGTTCAAGGGTTGTGATACACAGATGATTTATGACATGTT
  GCTAAGATTGAAATCCGTTCTCTATTTGCCTGGTGACTTTGTCTGCAAAAAGGGAGAAATTGGCAAGGAA
  ATGTATATCATCAAGCATGGAGAAGTCCAAGTTCTTGGAGGCCCTGATGGTACTAAAGTTCTGGTTACTC
  TGAAAGCTGGGTCGGTGTTTGGAGAAATCAGCCTTCTAGCAGCAGGAGGAGGAAACCGTCGAACTGCCAA
  TGTGGTGGCCCACGGGTTTGCCAATCTTTTAACTCTAGACAAAAAGACCCTCCAAGAAATTCTAGTGCAT
  TATCCAGATTCTGAAAGGATCCTCATGAAGAAAGCCAGAGTGCTTTTAAAGCAGAAGGCTAAGACCGCAG
  AAGCAACCCCTCCAAGAAAAGATCTTGCCCTCCTCTTCCCACCGAAAGAAGAGACACCCAAACTGTTTAA
  AACTCTCCTAGGAGGCACAGGAAAAGCAAGTCTTGCAAGACTACTCAAATTGAAGCGAGAGCAAGCAGCT
  CAGAAGAAAGAAAATTCTGAAGGAGGAGAGGAAGAAGGAAAAGAAAATGAAGATAAACAAAAAGAAAATG
  AAGATAAACAAAAAGAAAATGAAGATAAAGGAAAAGAAAATGAAGATAAAGATAAAGGAAGAGAGCCAGA
  AGAGAAGCCACTGGACAGACCTGAATGTACAGCAAGTCCTATTGCAGTGGAGGAAGAACCCCACTCAGTT
  AGAAGGACAGTTTTACCCAGAGGGACTTCTCGTCAATCACTCATTATCAGCATGGCTCCTTCTGCTGAGG
  GCGGAGAAGAGGTTCTTACTATTGAAGTCAAAGAAAAGGCTAAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212989 representing NM_019098
  Red=Cloning site Green=Tags(s)
  
  MFKSLTKVNKVKPIGENNENEQSSRRNEEGSHPSNQSQQTTAQEENKGEEKSLKTKSTPVTSEEPHTNIQ
  DKLSKKNSSGDLTTNPDPQNAAEPTGTVPEQKEMDPGKEGPNSPQNKPPAAPVINEYADAQLHNLVKRMR
  QRTALYKKKLVEGDLSSPEASPQTAKPTAVPPVKESDDKPTEHYYRLLWFKVKKMPLTEYLKRIKLPNSI
  DSYTDRLYLLWLLLVTLAYNWNCWFIPLRLVFPYQTADNIHYWLIADIICDIIYLYDMLFIQPRLQFVRG
  GDIIVDSNELRKHYRTSTKFQLDVASIIPFDICYLFFGFNPMFRANRMLKYTSFFEFNHHLESIMDKAYI
  YRVIRTTGYLLFILHINACVYYWASNYEGIGTTRWVYDGEGNEYLRCYYWAVRTLITIGGLPEPQTLFEI
  VFQLLNFFSGVFVFSSLIGQMRDVIGAATANQNYFRACMDDTIAYMNNYSIPKLVQKRVRTWYEYTWDSQ
  RMLDESDLLKTLPTTVQLALAIDVNFSIISKVDLFKGCDTQMIYDMLLRLKSVLYLPGDFVCKKGEIGKE
  MYIIKHGEVQVLGGPDGTKVLVTLKAGSVFGEISLLAAGGGNRRTANVVAHGFANLLTLDKKTLQEILVH
  YPDSERILMKKARVLLKQKAKTAEATPPRKDLALLFPPKEETPKLFKTLLGGTGKASLARLLKLKREQAA
  QKKENSEGGEEEGKENEDKQKENEDKQKENEDKGKENEDKDKGREPEEKPLDRPECTASPIAVEEEPHSV
  RRTVLPRGTSRQSLIISMAPSAEGGEEVLTIEVKEKAKQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019098
• ORF Size: 2427 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019098.2, NP_061971.2
• RefSeq Size: 4369 bp
• RefSeq ORF: 2430 bp
• Locus ID: 54714
• UniProt ID: Q9NQW8
• Cytogenetics: 8q21.3
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• Gene Summary: This gene encodes the beta subunit of a cyclic nucleotide-gated ion channel. The encoded beta subunit appears to play a role in modulation of channel function in cone photoreceptors. This heterotetrameric channel is necessary for sensory transduction, and mutations in this gene have been associated with achromatopsia 3, progressive cone dystrophy, and juvenile macular degeneration, also known as Stargardt Disease. [provided by RefSeq, Feb 2010]