D aspartate oxidase (DDO) (NM_004032) Human Tagged ORF Clone
CAT#: RC212769
DDO (Myc-DDK-tagged)-Human D-aspartate oxidase (DDO), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004032" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | D aspartate oxidase |
Synonyms | DASOX; DDO-1; DDO-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212769 representing NM_004032
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGGCTTCCAAGACTGCTTTT TCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGC TGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACCATCATTTCAGACAAGTTTACTCCAGATACC ACCAGTGATGTGGCAGCCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGC AGTGGTTCAGAGAAACCTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGT TCATTTGGTATCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAGAAGACCTGTGGGAA CTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCAGGAGACTCAA AGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTCAGGCTCCCTGGGTGGAGCATTTTATCCGAGATGG CAGTGGGCTGACATATATTTATCCTGGTACATCCCATGTAACCCTAGGTGGAACTAGGCAAAAAGGGGAC TGGAATCTGTCCCCGGATGCAGAAAATAGCAGAGAGATTCTTTCCCGATGCTGTGTTCTGGAGCCCTCCC TCCACGGAGCCTGCAACATCAGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGAC AGAGCTCCTTGCGCGAGATGGACAGAGGCTGCCTGTAGTCCACCACTATGGCCATGGGAGTGGGGGCATC TCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCATGCCCTCAGGACCC CCATTCCCAAGTCAAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212769 representing NM_004032
Red=Cloning site Green=Tags(s) MRPARHWETRFGARDFGGFQDCFFRDRLMDTARIAVVGAGVVGLSTAVCISKLVPRCSVTIISDKFTPDT TSDVAAGMLIPHTYPDTPIHTQKQWFRETFNHLFAIANSAEAGDAGVHLVSGIKGSGGWTLTRRIEDLWE LHPSFDIVVNCSGLGSRQLAGDSKIFPVRGQVLQVQAPWVEHFIRDGSGLTYIYPGTSHVTLGGTRQKGD WNLSPDAENSREILSRCCVLEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGI SVHWGTALEAARLVSECVHALRTPIPKSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004032 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004032.2, NP_004023.2 |
RefSeq Size | 1556 bp |
RefSeq ORF | 849 bp |
Locus ID | 8528 |
UniProt ID | Q99489 |
Cytogenetics | 6q21 |
Domains | DAO |
Protein Pathways | Alanine, aspartate and glutamate metabolism |
MW | 33.8 kDa |
Gene Summary | The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2019] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212769L3 | Lenti ORF clone of Human D-aspartate oxidase (DDO), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RC212769L4 | Lenti ORF clone of Human D-aspartate oxidase (DDO), transcript variant 2, mGFP tagged |
USD 600.00 |
|
RG212769 | DDO (tGFP-tagged) - Human D-aspartate oxidase (DDO), transcript variant 2 |
USD 500.00 |
|
SC117617 | DDO (untagged)-Human D-aspartate oxidase (DDO), transcript variant 2 |
USD 300.00 |
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