D aspartate oxidase (DDO) (NM_004032) Human Tagged ORF Clone

CAT#: RC212769

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DDO (Myc-DDK-tagged)-Human D-aspartate oxidase (DDO), transcript variant 2



  "NM_004032" in other vectors (4)

USD 433.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name D aspartate oxidase (DDO) (NM_004032) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DDO
Synonyms DASOX; DDO-1; DDO-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC212769 representing NM_004032
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGGCTTCCAAGACTGCTTTT
TCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGC
TGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACCATCATTTCAGACAAGTTTACTCCAGATACC
ACCAGTGATGTGGCAGCCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGC
AGTGGTTCAGAGAAACCTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGT
TCATTTGGTATCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAGAAGACCTGTGGGAA
CTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCAGGAGACTCAA
AGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTCAGGCTCCCTGGGTGGAGCATTTTATCCGAGATGG
CAGTGGGCTGACATATATTTATCCTGGTACATCCCATGTAACCCTAGGTGGAACTAGGCAAAAAGGGGAC
TGGAATCTGTCCCCGGATGCAGAAAATAGCAGAGAGATTCTTTCCCGATGCTGTGTTCTGGAGCCCTCCC
TCCACGGAGCCTGCAACATCAGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGAC
AGAGCTCCTTGCGCGAGATGGACAGAGGCTGCCTGTAGTCCACCACTATGGCCATGGGAGTGGGGGCATC
TCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCATGCCCTCAGGACCC
CCATTCCCAAGTCAAACCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212769 representing NM_004032
Red=Cloning site Green=Tags(s)

MRPARHWETRFGARDFGGFQDCFFRDRLMDTARIAVVGAGVVGLSTAVCISKLVPRCSVTIISDKFTPDT
TSDVAAGMLIPHTYPDTPIHTQKQWFRETFNHLFAIANSAEAGDAGVHLVSGIKGSGGWTLTRRIEDLWE
LHPSFDIVVNCSGLGSRQLAGDSKIFPVRGQVLQVQAPWVEHFIRDGSGLTYIYPGTSHVTLGGTRQKGD
WNLSPDAENSREILSRCCVLEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGI
SVHWGTALEAARLVSECVHALRTPIPKSNL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004032
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004032.2, NP_004023.2
RefSeq Size 1556 bp
RefSeq ORF 849 bp
Locus ID 8528
Cytogenetics 6q21
Domains DAO
Protein Pathways Alanine, aspartate and glutamate metabolism
MW 33.8 kDa
Gene Summary The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2019]

Other Versions

Frequently bought together (3)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

USD 350.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 570.00


Rabbit Polyclonal Anti-DDO Antibody
    • 100 ul

USD 360.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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