GLDC (NM_000170) Human Tagged ORF Clone

CAT#: RC211292

GLDC (Myc-DDK-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC211292

• 

  Western blot validation of overexpression lysate (Cat# LY424873) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211292 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLDC
• Synonyms: GCE; GCSP; HYGN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211292 representing NM_000170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCCTGTGCCAGGGCGTGGGGGCTGCGCCTGGGCCGCGGGGTCGGGGGCGGCCGCCGCCTGGCTG
  GGGGATCGGGGCCGTGCTGGGCGCCGCGGAGCCGGGACAGCAGCAGTGGCGGCGGGGACAGCGCCGCGGC
  TGGGGCCTCGCGCCTCCTGGAGCGCCTTCTGCCCAGACACGACGACTTCGCTCGGAGGCACATCGGCCCT
  GGGGACAAAGACCAGAGAGAGATGCTGCAGACCTTGGGGCTGGCGAGCATTGATGAATTGATCGAGAAGA
  CGGTCCCTGCCAACATCCGTTTGAAAAGACCCTTGAAAATGGAAGACCCTGTTTGTGAAAATGAAATCCT
  TGCAACTCTGCATGCCATTTCAAGCAAAAACCAGATCTGGAGATCGTATATTGGCATGGGCTATTATAAC
  TGCTCAGTGCCACAGACGATTTTGCGGAACTTACTGGAGAACTCAGGATGGATCACCCAGTATACTCCAT
  ACCAGCCTGAGGTGTCTCAGGGGAGGCTGGAGAGTTTACTCAACTACCAGACCATGGTGTGTGACATCAC
  AGGCCTGGACATGGCCAATGCATCCCTGCTGGATGAGGGGACTGCAGCCGCAGAGGCACTGCAGCTGTGC
  TACAGACACAACAAGAGGAGGAAATTTCTCGTTGATCCCCGTTGCCACCCACAGACAATAGCTGTTGTCC
  AGACTCGAGCCAAATATACTGGAGTCCTCACTGAGCTGAAGTTACCCTGTGAAATGGACTTCAGTGGAAA
  AGATGTCAGTGGAGTGTTGTTCCAGTACCCAGACACGGAGGGGAAGGTGGAAGACTTTACGGAACTCGTG
  GAGAGAGCTCATCAGAGTGGGAGCCTGGCCTGCTGTGCTACTGACCTTTTAGCTTTGTGCATCTTGAGGC
  CACCTGGAGAATTTGGGGTAGACATCGCCCTGGGCAGCTCCCAGAGATTTGGAGTGCCACTGGGCTATGG
  GGGACCCCATGCAGCATTTTTTGCTGTCCGAGAAAGCTTGGTGAGAATGATGCCTGGAAGAATGGTGGGG
  GTAACAAGAGATGCCACTGGGAAAGAAGTGTATCGTCTTGCTCTTCAAACCAGGGAGCAACACATTCGGA
  GAGACAAGGCTACCAGCAACATCTGTACAGCTCAGGCCCTCTTGGCGAATATGGCTGCCATGTTTGCAAT
  CTACCATGGTTCCCATGGGCTGGAGCATATTGCTAGGAGGGTACATAATGCCACTTTGATTTTGTCAGAA
  GGTCTCAAGCGAGCAGGGCATCAACTCCAGCATGACCTGTTCTTTGATACCTTGAAGATTCAGTGTGGCT
  GCTCAGTGAAGGAGGTCTTGGGCAGGGCCGCTCAGCGGCAGATCAATTTTCGGCTTTTTGAGGATGGCAC
  ACTTGGTATTTCTCTTGATGAAACAGTCAATGAAAAAGATCTGGACGATTTGTTGTGGATCTTTGGTTGT
  GAGTCATCTGCAGAACTGGTTGCTGAAAGCATGGGAGAGGAGTGCAGAGGTATTCCAGGGTCTGTGTTCA
  AGAGGACCAGCCCGTTCCTCACCCATCAAGTGTTCAACAGCTACCACTCTGAAACAAACATTGTCCGGTA
  CATGAAGAAACTGGAAAATAAAGACATTTCCCTTGTTCACAGCATGATTCCACTGGGATCCTGCACCATG
  AAACTGAACAGTTCGTCTGAACTCGCACCTATCACATGGAAAGAATTTGCAAACATCCACCCCTTTGTGC
  CTCTGGATCAAGCTCAAGGATATCAGCAGCTTTTCCGAGAGCTTGAGAAGGATTTGTGTGAACTCACAGG
  TTATGACCAGGTCTGTTTCCAGCCAAACAGCGGAGCCCAGGGAGAATATGCTGGACTGGCCACTATCCGA
  GCCTACTTAAACCAGAAAGGAGAGGGGCACAGAACGGTTTGCCTCATTCCGAAATCAGCACATGGGACCA
  ACCCAGCAAGTGCCCACATGGCAGGCATGAAGATTCAGCCTGTGGAGGTGGATAAATATGGGAATATCGA
  TGCAGTTCACCTCAAGGCCATGGTGGATAAGCACAAGGAGAACCTAGCAGCTATCATGATTACATACCCA
  TCCACCAATGGGGTGTTTGAAGAGAACATCAGTGACGTGTGTGACCTCATCCATCAACATGGAGGACAGG
  TCTACCTAGACGGGGCAAATATGAATGCTCAGGTGGGAATCTGTCGCCCTGGAGACTTCGGGTCTGATGT
  CTCGCACCTAAATCTTCACAAGACCTTCTGCATTCCCCACGGAGGAGGTGGTCCTGGCATGGGGCCCATC
  GGAGTGAAGAAACATCTCGCCCCGTTTTTGCCCAATCATCCCGTCATTTCACTAAAGCGGAATGAGGATG
  CCTGTCCTGTGGGAACCGTCAGTGCGGCCCCATGGGGCTCCAGTTCCATCTTGCCCATTTCCTGGGCTTA
  TATCAAGATGATGGGAGGCAAGGGTCTTAAACAAGCCACGGAAACTGCGATATTAAATGCCAACTACATG
  GCCAAGCGATTAGAAACACACTACAGAATTCTTTTCAGGGGTGCAAGAGGTTATGTGGGTCATGAATTTA
  TTTTGGACACGAGACCCTTCAAAAAGTCTGCAAATATTGAGGCTGTGGATGTGGCCAAGAGACTCCAGGA
  TTATGGATTTCACGCCCCTACCATGTCCTGGCCTGTGGCAGGGACCCTCATGGTGGAGCCCACTGAGTCG
  GAGGACAAGGCAGAGCTGGACAGATTCTGTGATGCCATGATCAGCATTCGGCAGGAAATTGCTGACATTG
  AGGAGGGCCGCATCGACCCCAGGGTCAATCCGCTGAAGATGTCTCCACACTCCCTGACCTGCGTTACATC
  TTCCCACTGGGACCGGCCTTATTCCAGAGAGGTGGCAGCATTCCCACTCCCCTTCGTGAAACCAGAGAAC
  AAATTCTGGCCAACGATTGCCCGGATTGATGACATATATGGAGATCAGCACCTGGTTTGTACCTGCCCAC
  CCATGGAAGTTTATGAGTCTCCATTTTCTGAACAAAAGAGGGCGTCTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211292 representing NM_000170
  Red=Cloning site Green=Tags(s)
  
  MQSCARAWGLRLGRGVGGGRRLAGGSGPCWAPRSRDSSSGGGDSAAAGASRLLERLLPRHDDFARRHIGP
  GDKDQREMLQTLGLASIDELIEKTVPANIRLKRPLKMEDPVCENEILATLHAISSKNQIWRSYIGMGYYN
  CSVPQTILRNLLENSGWITQYTPYQPEVSQGRLESLLNYQTMVCDITGLDMANASLLDEGTAAAEALQLC
  YRHNKRRKFLVDPRCHPQTIAVVQTRAKYTGVLTELKLPCEMDFSGKDVSGVLFQYPDTEGKVEDFTELV
  ERAHQSGSLACCATDLLALCILRPPGEFGVDIALGSSQRFGVPLGYGGPHAAFFAVRESLVRMMPGRMVG
  VTRDATGKEVYRLALQTREQHIRRDKATSNICTAQALLANMAAMFAIYHGSHGLEHIARRVHNATLILSE
  GLKRAGHQLQHDLFFDTLKIQCGCSVKEVLGRAAQRQINFRLFEDGTLGISLDETVNEKDLDDLLWIFGC
  ESSAELVAESMGEECRGIPGSVFKRTSPFLTHQVFNSYHSETNIVRYMKKLENKDISLVHSMIPLGSCTM
  KLNSSSELAPITWKEFANIHPFVPLDQAQGYQQLFRELEKDLCELTGYDQVCFQPNSGAQGEYAGLATIR
  AYLNQKGEGHRTVCLIPKSAHGTNPASAHMAGMKIQPVEVDKYGNIDAVHLKAMVDKHKENLAAIMITYP
  STNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGPGMGPI
  GVKKHLAPFLPNHPVISLKRNEDACPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKQATETAILNANYM
  AKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTES
  EDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPEN
  KFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000170
• ORF Size: 3060 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000170.3
• RefSeq Size: 3783 bp
• RefSeq ORF: 3063 bp
• Locus ID: 2731
• UniProt ID: P23378
• Cytogenetics: 9p24.1
• Domains: GDC-P
• Protein Families: Druggable Genome
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• MW: 112.73 kDa
• Gene Summary: Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]