IRX4 (NM_016358) Human Tagged ORF Clone

CAT#: RC211129

IRX4 (Myc-DDK-tagged)-Human iroquois homeobox 4 (IRX4)

Product Images

Circular map for RC211129

• 

  Western blot validation of overexpression lysate (Cat# LY414026) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211129 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRX4
• Synonyms: IRXA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211129 representing NM_016358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTACCCGCAGTTTGGATACCCCTACTCCTCGGCTCCCCAGTTCTTGATGGCCACCAACTCCCTGA
  GCACGTGCTGCGAGTCCGGAGGCCGCACGCTGGCGGACTCCGGGCCCGCCGCCTCGGCCCAGGCGCCGGT
  CTACTGCCCGGTCTACGAGAGCCGGCTGCTGGCCACCGCGCGCCACGAGCTCAACTCGGCCGCGGCGCTG
  GGCGTCTATGGGGGTCCCTATGGCGGATCGCAGGGCTATGGCAACTACGTGACCTACGGCTCGGAGGCGT
  CCGCCTTCTACTCGCTGAACAGCTTTGATTCCAAGGATGGTTCGGGATCTGCGCATGGGGGCCTGGCACC
  AGCCACTGCCGCCTACTACCCTTACGAGCCAGCTCTGGGCCAGTACCCCTATGACAGGTATGGAACCATG
  GACAGCGGCACGCGGCGCAAGAACGCCACGCGCGAGACCACCAGCACGCTCAAGGCCTGGCTGCAGGAGC
  ACCGCAAGAACCCCTACCCCACCAAGGGCGAGAAGATCATGCTGGCCATCATCACCAAGATGACCCTCAC
  ACAGGTCTCCACCTGGTTCGCCAACGCGCGCCGGCGCCTCAAGAAGGAGAACAAGATGACGTGGCCGCCG
  CGGAACAAGTGCGCAGACGAGAAGCGGCCCTACGCGGAGGGCGAGGAGGAGGAGGGGGGCGAGGAGGAGG
  CGCGGGAGGAGCCCCTCAAGAGCTCCAAGAACGCAGAGCCCGTGGGCAAAGAGGAGAAGGAGCTGGAGCT
  TAGTGACTTGGACGACTTCGACCCGCTGGAAGCAGAGCCGCCGGCGTGCGAGCTGAAGCCGCCCTTCCAC
  TCCCTGGACGGCGGTCTGGAGCGCGTCCCCGCCGCGCCCGACGGCCCGGTCAAGGAGGCCTCAGGCGCGC
  TCCGGATGTCTCTGGCCGCGGGTGGCGGAGCTGCTCTGGACGAGGACCTGGAGAGGGCCCGGAGCTGTCT
  CCGCAGCGCGGCGGCCGGGCCGGAGCCACTGCCGGGCGCAGAGGGCGGCCCTCAGGTCTGCGAGGCCAAG
  CTGGGGTTTGTGCCGGCGGGGGCGTCGGCAGGCCTGGAGGCTAAGCCGCGCATCTGGTCCCTGGCCCACA
  CAGCCACCGCCGCCGCCGCCGCCGCCACCTCCCTGAGCCAGACTGAGTTTCCGTCGTGCATGCTCAAGCG
  CCAAGGTCCCGCGGCCCCTGCGGCTGTGTCCTCCGCGCCCGCCACGTCCCCGTCTGTGGCCCTTCCCCAC
  TCTGGCGCCCTGGACAGGCACCAGGACTCCCCGGTAACCAGTCTCAGAAACTGGGTGGACGGGGTCTTCC
  ACGACCCCATCCTCAGGCACAGCACTTTGAACCAGGCCTGGGCCACCGCCAAGGGCGCCCTCCTGGACCC
  CGGGCCTCTGGGACGCTCGCTGGGGGCGGGCGCGAACGTGCTGACTGCACCCCTGGCCCGCGCCTTTCCG
  CCTGCCGTGCCCCAGGACGCCCCAGCTGCAGGCGCCGCCAGGGAGCTGCTCGCCCTGCCCAAGGCCGGCG
  GCAAACCCTTCTGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211129 representing NM_016358
  Red=Cloning site Green=Tags(s)
  
  MSYPQFGYPYSSAPQFLMATNSLSTCCESGGRTLADSGPAASAQAPVYCPVYESRLLATARHELNSAAAL
  GVYGGPYGGSQGYGNYVTYGSEASAFYSLNSFDSKDGSGSAHGGLAPATAAYYPYEPALGQYPYDRYGTM
  DSGTRRKNATRETTSTLKAWLQEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWPP
  RNKCADEKRPYAEGEEEEGGEEEAREEPLKSSKNAEPVGKEEKELELSDLDDFDPLEAEPPACELKPPFH
  SLDGGLERVPAAPDGPVKEASGALRMSLAAGGGAALDEDLERARSCLRSAAAGPEPLPGAEGGPQVCEAK
  LGFVPAGASAGLEAKPRIWSLAHTATAAAAAATSLSQTEFPSCMLKRQGPAAPAAVSSAPATSPSVALPH
  SGALDRHQDSPVTSLRNWVDGVFHDPILRHSTLNQAWATAKGALLDPGPLGRSLGAGANVLTAPLARAFP
  PAVPQDAPAAGAARELLALPKAGGKPFCA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016358
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016358.3
• RefSeq Size: 2212 bp
• RefSeq ORF: 1560 bp
• Locus ID: 50805
• UniProt ID: P78413
• Cytogenetics: 5p15.33
• MW: 54.3 kDa
• Gene Summary: Likely to be an important mediator of ventricular differentiation during cardiac development.[UniProtKB/Swiss-Prot Function]