IRX4 (NM_016358) Human Untagged Clone

CAT#: SC322077

IRX4 (untagged)-Human iroquois homeobox 4 (IRX4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRX4
• Synonyms: IRXA3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016358, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCTACCCGCAGTTTGGATACCCCTACTCCTCGGCTCCCCAGTTCTTGATGGCCACCAACTCCCTGA
  GCACGTGCTGCGAGTCCGGAGGCCGCACGCTGGCGGACTCCGGGCCCGCCGCCTCGGCCCAGGCGCCGGT
  CTACTGCCCGGTCTACGAGAGCCGGCTGCTGGCCACCGCGCGCCACGAGCTCAACTCGGCCGCGGCGCTG
  GGCGTCTATGGGGGTCCCTATGGCGGATCGCAGGGCTATGGCAACTACGTGACCTACGGCTCGGAGGCGT
  CCGCCTTCTACTCGCTGAACAGCTTTGATTCCAAGGATGGTTCGGGATCTGCGCATGGGGGCCTGGCACC
  AGCCGCTGCCGCCTACTACCCTTACGAGCCAGCTCTGGGCCAGTACCCCTATGACAGGTATGGAACCATG
  GACAGCGGCACGCGGCGCAAGAACGCCACGCGCGAGACCACCAGCACGCTCAAGGCCTGGCTGCAGGAGC
  ACCGCAAGAACCCCTACCCCACCAAGGGCGAGAAGATCATGCTGGCCATCATCACCAAGATGACCCTCAC
  ACAGGTCTCCACCTGGTTCGCCAACGCGCGCCGGCGCCTCAAGAAGGAGAACAAGATGACGTGGCCGCCG
  CGGAACAAGTGCGCAGACGAGAAGCGGCCCTACGCGGAGGGCGAGGAGGAGGAGGGGGGCGAGGAGGAGG
  CGCGGGAGGAGCCCCTCAAGAGCTCCAAGAACGCAGAGCCCGTGGGCAAAGAGGAGAAGGAGCTGGAGCT
  TAGTGACTTGGACGACTTCGACCCGCTGGAAGCAGAGCCGCCGGCGTGCGAGCTGAAGCCGCCCTTCCAC
  TCCCTGGACGGCGGTCTGGAGCGCGTCCCCGCCGCGCCCGACGGCCCGGTCAAGGAGGCCTCAGGCGCGC
  TCCGGATGTCTCTGGCCGCGGGTGGCGGAGCTGCTCTGGACGAGGACCTGGAGAGGGCCCGGAGCTGTCT
  CCGCAGCGCGGCGGCCGGGCCGGAGCCACTGCCGGGCGCAGAGGGCGGCCCTCAGGTCTGCGAGGCCAAG
  CTGGGGTTTGTGCCGGCGGGGGCGTCGGCAGGCCTGGAGGCTAAGCCGCGCATCTGGTCCCTGGCCCACA
  CAGCCACCGCCGCCGCCGCCGCCGCCACCTCCCTGAGCCAGACTGAGTTTCCGTCGTGCATGCTCAAGCG
  CCAAGGTCCCGCGGCCCCTGCGGCTGTGTCCTCCGCGCCCGCCACGTCCCCGTCTGTGGCCCTTCCCCAC
  TCTGGCGCCCTGGACAGGCACCAGGACTCCCCGGTAACCAGTCTCAGAAACTGGGTGGACGGGGTCTTCC
  ACGACCCCATCCTCAGGCACAGCACTTTGAACCAGGCCTGGGCCACCGCCAAGGGCGCCCTCCTGGACCC
  CGGGCCTCTGGGACGCTCGCTGGGGGCGGGCGCGAACGTGCTGACTGCACCCCTGGCCCGCGCCTTTCCG
  CCTGCCGTGCCCCAGGACGCCCCAGCTGCAGGCGCCGCCAGGGAGCTGCTCGCCCTGCCCAAGGCCGGCG
  GCAAACCCTTCTGCGCCTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_016358
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016358.1, NP_057442.1
• RefSeq Size: 2212 bp
• RefSeq ORF: 1560 bp
• Locus ID: 50805
• UniProt ID: P78413
• Cytogenetics: 5p15.33
• Gene Summary: Likely to be an important mediator of ventricular differentiation during cardiac development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR and lacks an alternate internal exon compared to variant 4. It encodes isoform b which is shorter than isoform a.