BDH2 (NM_020139) Human Tagged ORF Clone

CAT#: RC210586

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BDH2 (Myc-DDK-tagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2)



  "NM_020139" in other vectors (4)

Reconstitution Protocol

USD 300.00

USD 450.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol BDH2
Synonyms DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210586 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCGACTTGATGGGAAAGTCATCATCCTGACGGCCGCTGCTCAGGGGATTGGCCAAGCAGCTGCCT
TAGCTTTTGCAAGAGAAGGTGCCAAAGTCATAGCCACAGACATTAATGAGTCCAAACTTCAGGAACTGGA
AAAGTACCCGGGTATTCAAACTCGTGTCCTTGATGTCACAAAGAAGAAACAAATTGATCAGTTTGCCAAT
GAAGTTGAGAGACTTGATGTTCTCTTTAATGTTGCTGGTTTTGTCCATCATGGAACTGTCCTGGATTGTG
AGGAGAAAGACTGGGACTTCTCGATGAATCTCAATGTGCGCAGCATGTACCTGATGATCAAGGCATTCCT
TCCTAAAATGCTTGCTCAGAAATCTGGCAATATTATCAACATGTCTTCTGTGGCTTCCAGCGTCAAAGGA
GTTGTGAACAGATGTGTGTACAGCACAACCAAGGCAGCCGTGATTGGCCTCACAAAATCTGTGGCTGCAG
ATTTCATCCAGCAGGGCATCAGGTGCAACTGTGTGTGCCCAGGAACAGTTGATACGCCATCTCTACAAGA
AAGAATACAAGCCAGAGGAAATCCTGAAGAGGCACGGAATGATTTCCTGAAGAGACAAAAGACGGGAAGA
TTCGCAACTGCAGAAGAAATAGCCATGCTCTGCGTGTATTTGGCTTCTGATGAATCTACTTATGTAACTG
GTAACCCTGTCATCATTGATGGAGGCTGGAGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210586 protein sequence
Red=Cloning site Green=Tags(s)

MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFAN
EVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKG
VVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGR
FATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020139
ORF Size 735 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020139.4
RefSeq Size 2936 bp
RefSeq ORF 738 bp
Locus ID 56898
UniProt ID Q9BUT1
Cytogenetics 4q24
Domains adh_short
Protein Families Druggable Genome
Protein Pathways Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
MW 26.8 kDa
Gene Summary Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity). Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).[UniProtKB/Swiss-Prot Function]

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