BDH2 (NM_020139) Human Untagged Clone

CAT#: SC113190

BDH2 (untagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDH2
• Synonyms: DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113190 sequence for NM_020139 edited (data generated by NextGen Sequencing)
  ATGGGTCGACTTGATGGGAAAGTCATCATCCTGACGGCCGCTGCTCAGGGGATTGGCCAA
  GCAGCTGCCTTAGCTTTTGCAAGAGAAGGTGCCAAAGTCATAGCCACAGACATTAATGAG
  TCCAAACTTCAGGAACTGGAAAAGTACCCGGGTATTCAAACTCGTGTCCTTGATGTCACA
  AAGAAGAAACAAATTGATCAGTTTGCCAATGAAGTTGAGAGACTTGATGTTCTCTTTAAT
  GTTGCTGGTTTTGTCCATCATGGAACTGTCCTGGATTGTGAGGAGAAAGACTGGGACTTC
  TCGATGAATCTCAATGTGCGCAGCATGTACCTGATGATCAAGGCATTCCTTCCTAAAATG
  CTTGCTCAGAAATCTGGCAATATTATCAACATGTCTTCTGTGGCTTCCAGCGTCAAAGGA
  GTTGTGAACAGATGTGTGTACAGCACAACCAAGGCAGCCGTGATTGGCCTCACAAAATCT
  GTGGCTGCAGATTTCATCCAGCAGGGCATCAGGTGCAACTGTGTGTGCCCAGGAACAGTT
  GATACGCCATCTCTACAAGAAAGAATACAAGCCAGAGGAAATCCTGAAGAGGCACGGAAT
  GATTTCCTGAAGAGACAAAAGACGGGAAGATTCGCAACTGCAGAAGAAATAGCCATGCTC
  TGCGTGTATTTGGCTTCTGATGAATCTGCTTATGTAACTGGTAACCCTGTCATCATTGAT
  GGAGGCTGGAGCTTGTGA
  
  Clone variation with respect to NM_020139.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020139 unedited
  CCCCATTTATGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGTTCTGGA
  GAACGCCATCAGCTCGCTGCTTAAAATTAAACCACAGGTTCCATTATGGGTCGACTTGAT
  GGGAAAGTCATCATCCTGACGGCCGCTGCTCAGGGGATTGGCCAAGCAGCTGCCTTAGCT
  TTTGCAAGAGAAGGTGCCAAAGTCATAGCCACAGACATTAATGAGTCCAAACTTCAGGAA
  CTGGAAAAGTACCCGGGTATTCAAACTCGTGTCCTTGATGTCACAAAGAAGAAACAAATT
  GATCAGTTTGCCAATGAAGTTGAGAGACTTGATGTTCTCTTTAATGTTGCTGGTTTTGTC
  CATCATGGAACTGTCCTGGATTGTGAGGAGAAAGACTGGGACTTCTCGATGAATCTCAAT
  GTGCGCAGCATGTACCTGATGATCAAGGCATTCCTTCCTAAAATGCTTGCTCAGAAATCT
  GGCAATATTATCAACATGTCTTCTGTGGCTTCCAGCGTCAAAGGTGGGTCTGTCTCCTTC
  CGAGGACTGCGATGCTCATACACGCACATCATTAAGAGCTCTGCGTTTGGGAACAGGCAT
  AGCAGAGATTATAATTTCAAGTATTGAAATGATTTCAAAACTGCTTTTTTCAAAATTGGT
  ATTAAGTTCCTTAACCACAGATCTTTTGCTCTCGATGTGAGCCAGTGGTAAAATTAAATT
  AAAATGTGGGGTATTTTTGCCCTCCCTTTTATTCTTTCTAATGGACATGGAAATGAACAT
  CAAACTGGGAGAAAGAAACCATTTACATTTAATTAATTTAAAATAGTGTATTGAGCACCG
  GTATGTGCTCTGGCCATAAAGAATCACAGTCCAAACTAGGAGCAGGCAGCAACATCANTT
  CTCCATGTGATGAATAAACAGAGGTTGTCAAGCGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020139 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTCAATATTTTTATT
  TCTTTACAATGATTTCAGAAGAGATTACAAAGAGATTAATATACTTAAAGAATCAGACTC
  TTGCAAACAGTGACATCATTAAAAAGAGCTTATTTTCATTAACATGTGATTAACAGGAAG
  GAGATGATTGGTGAGTTTTCTTCGTAACCAGGTTCACTGTGGATAGGAAGGGCCTGCCTT
  CCTTCCCACCATGGAGATCCTAAAATCACAAGCTCCAGCCTCCATCAATGATGACAGGGT
  TACCAGTTACATAAGCAGATTCATCAGAAGCCAAATACACGCAGAGCATGGCTATTTCTT
  CTGCAGTTGCGAATCTTCCCGTCTTTTGTCTCTTCAGGAAATCATTCCGTGCCTCTTCAG
  GATTTCCTCTGGCTTGTATTCTTTCTTGTAGAGATGGCGTATCAACTGTTCCTGGGCACA
  CACAGTTGCACCTGATGCCCTGCTGGATGAAATCTGCAGCCACAGATTTTGTGAGGCCAA
  TCACGGCTGCCTTGGTTGTGCTGTACACACATCTGTTCACAACTCCTGAGGGTGGAGGAA
  AGGTTCAACCATTGAAGGAATAAAAAATGTATTTTCCTTCAGGAAAGGTACACAAATAAG
  TTTTCCCAGTAGCCATTTTCATGCTTTCTTCCCACATTCCTCCCCTTTTAAAAGATTGAA
  TCAGATTTTTTCCTTCATAGAGTCAGTCTCTGATCTAGGAAAAATATCAGTATAGCACAC
  TTATTGAGACTTAATGAATGCTGAACTACTTAAATGGGAGCCTTAGTTCTATCATCAGTG
  CCTACTCTTAGCCGATTCAAGGAAATGAGATTTTACCAACCAACACTAACCGGAGGCTAG
  CCCTTCTTACTTCCTCTGGGAGGAGAAAGGGAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020139
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020139.3, NP_064524.3
• RefSeq Size: 2936 bp
• RefSeq ORF: 738 bp
• Locus ID: 56898
• UniProt ID: Q9BUT1
• Cytogenetics: 4q24
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
• Gene Summary: Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity). Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).[UniProtKB/Swiss-Prot Function]