ARMC9 (NM_025139) Human Tagged ORF Clone

CAT#: RC209499

ARMC9 (Myc-DDK-tagged)-Human armadillo repeat containing 9 (ARMC9)

Product Images

Circular map for RC209499

• 

  Western blot validation of overexpression lysate (Cat# LY410879) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209499 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ARMC9 protein (Cat# TP309499). The protein was produced from HEK293T cells transfected with ARMC9 cDNA clone (Cat# RC209499) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMC9
• Synonyms: ARM; JBTS30; KU-MEL-1; NS21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209499 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACATTCTGGCTCATGAATCTGAATTACTTGGACTAGTGAAAGAGTATTTAGATTTTGCTGAAT
  TTGAAGACACCTTGAAAACATTTTCAAAAGAATGCAAAATAAAAGGAAAACCACTGTGTAAAACAGTAGG
  CGGATCTTTCAGAGACTCCAAATCATTGACAATTCAGAAGGATCTTGTCGCTGCATTTGACAACGGAGAC
  CAGAAGGTGTTCTTCGATCTGTGGGAGGAGCACATTTCAAGTTCCATCCGAGATGGGGACTCCTTTGCCC
  AGAAGCTGGAATTCTATCTCCACATCCATTTTGCCATCTATTTTTTGAAGTACTCTGTGGGGAGACCGGA
  CAAAGAGGAGCTGGATGAAAAGATTTCCTACTTCAAAACCTACCTGGAGACCAAAGGGGCAGCCTTGAGC
  CAGACCACAGAGTTTCTTCCTTTCTATGCCCTTCCTTTTGTTCCCAACCCTATGGTGCACCCCTCATTTA
  AAGAACTCTTCCAGGATTCCTGGACTCCAGAGTTAAAGTTGAAGTTGGAAAAGTTTCTAGCTTTAATATC
  TAAAGCCAGCAACACGCCAAAGCTTTTAACAATATATAAGGAGAATGGACAAAGTAACAAAGAAATCTTG
  CAGCAGCTCCACCAGCAGCTGGTTGAAGCTGAACGTAGGTCAGTGACATACCTCAAACGGTACAATAAGA
  TCCAGGCCGACTACCACAATCTCATTGGAGTCACAGCAGAGCTGGTGGATTCTCTAGAGGCCACAGTCAG
  CGGCAAGATGATCACCCCTGAGTACCTCCAGAGCGTCTGTGTCCGCCTGTTCAGTAACCAGATGCGGCAG
  AGCCTGGCGCATAGTGTGGACTTCACGAGGCCTGGGACGGCATCCACCATGTTACGAGCCTCCTTGGCAC
  CCGTGAAATTGAAGGATGTCCCATTACTGCCCTCCTTGGATTATGAGAAACTGAAGAAGGATTTGATTTT
  GGGGAGTGACCGCTTGAAAGCCTTCTTGTTGCAGGCTCTGCGCTGGCGCTTGACCACATCCCATCCTGGA
  GAGCAGAGGGAGACCGTTCTGCAAGCCTACATCAGCAATGACCTCTTGGACTGTTATAGCCACAACCAGA
  GGAGTGTGCTTCAGTTGCTGCACTCCACGAGCGACGTGGTGCGGCAGTACATGGCCAGGCTCATCAATGC
  TTTTGCGTCACTGGCAGAAGGTCGCCTCTACCTTGCCCAGAACACAAAGGTGCTGCAGATGCTGGAGGGA
  AGGCTGAAGGAGGAGGACAAGGATATCATCACCAGGGAGAATGTTCTTGGGGCCCTGCAGAAGTTCAGTC
  TCAGGCGCCCGCTGCAGACAGCGATGATTCAAGACGGCCTCATCTTCTGGCTGGTTGATGTTCTGAAGGA
  CCCTGACTGCCTGTCTGACTACACGCTGGAGTACTCGGTGGCTTTGCTCATGAACCTCTGCCTCCGCAGC
  ACAGGGAAGAACATGTGTGCCAAGGTGGCAGGCCTCGTGCTCAAAGTCCTTTCGGATCTTCTTGGCCATG
  AAAACCATGAGATACAGCCGTATGTGAATGGAGCTCTGTACAGCATCCTTTCTGTTCCATCCATTCGTGA
  GGAAGCAAGAGCAATGGGAATGGAAGACATCCTACGCTGCTTCATCAAAGAAGGCAATGCTGAAATGATC
  CGCCAGATAGAATTCATCATCAAGCAGCTAAATTCCGAAGAGCTACCAGATGGTGTTCTTGAATCTGATG
  ATGATGAAGATGAAGATGATGAAGAGGACCATGACATCATGGAAGCCGATCTGGACAAAGACGAACTGAT
  CCAGCCCCAGCTCGGAGAACTCTCAGGAGAGAAGCTTCTGACCACGGAGTACCTGGGGATCATGACCAAC
  ACGGGGAAGACAAGGCGGAAGGGGCTGGCTAATGTGCAGTGGAGCGGGGATGAGCCCCTGCAAAGGCCCG
  TCACCCCCGGCGGCCACAGAAACGGGTACCCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209499 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDILAHESELLGLVKEYLDFAEFEDTLKTFSKECKIKGKPLCKTVGGSFRDSKSLTIQKDLVAAFDNGD
  QKVFFDLWEEHISSSIRDGDSFAQKLEFYLHIHFAIYFLKYSVGRPDKEELDEKISYFKTYLETKGAALS
  QTTEFLPFYALPFVPNPMVHPSFKELFQDSWTPELKLKLEKFLALISKASNTPKLLTIYKENGQSNKEIL
  QQLHQQLVEAERRSVTYLKRYNKIQADYHNLIGVTAELVDSLEATVSGKMITPEYLQSVCVRLFSNQMRQ
  SLAHSVDFTRPGTASTMLRASLAPVKLKDVPLLPSLDYEKLKKDLILGSDRLKAFLLQALRWRLTTSHPG
  EQRETVLQAYISNDLLDCYSHNQRSVLQLLHSTSDVVRQYMARLINAFASLAEGRLYLAQNTKVLQMLEG
  RLKEEDKDIITRENVLGALQKFSLRRPLQTAMIQDGLIFWLVDVLKDPDCLSDYTLEYSVALLMNLCLRS
  TGKNMCAKVAGLVLKVLSDLLGHENHEIQPYVNGALYSILSVPSIREEARAMGMEDILRCFIKEGNAEMI
  RQIEFIIKQLNSEELPDGVLESDDDEDEDDEEDHDIMEADLDKDELIQPQLGELSGEKLLTTEYLGIMTN
  TGKTRRKGLANVQWSGDEPLQRPVTPGGHRNGYPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025139
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025139.6
• RefSeq Size: 2317 bp
• RefSeq ORF: 1998 bp
• Locus ID: 80210
• UniProt ID: Q7Z3E5
• Cytogenetics: 2q37.1
• MW: 75.6 kDa
• Gene Summary: Required for ciliogenesis.[UniProtKB/Swiss-Prot Function]