DAGK (DGKQ) (NM_001347) Human Tagged ORF Clone

CAT#: RC209068

DGKQ (Myc-DDK-tagged)-Human diacylglycerol kinase, theta 110kDa (DGKQ)

Product Images

Circular map for RC209068

• 

  Western blot validation of overexpression lysate (Cat# LY419993) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209068 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAGK
• Synonyms: DAGK; DAGK4; DAGK7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209068 representing NM_001347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCTGGCTGGGCGGCGGCTCCCCGCGCCCCGGCAGCCCGG
  CCTGCAGCCTCGTGCTGGGCTCAGGAGGCCGCGCGCGCCCGGGGCCGGGGCCGGGGCCGGGACCCGAGCG
  GGCGGGCGTCAGAGCCCCGGGCCCCGCTGCCGCGCCGGGACACAGCTTCCGGAAGGTGACGCTCACCAAG
  CCCACCTTCTGCCACCTCTGCTCCGACTTCATCTGGGGGCTGGCCGGCTTCCTGTGCGACGTCTGCAATT
  TCATGTCTCATGAGAAGTGCCTGAAGCACGTGAGGATCCCGTGCACGAGTGTGGCACCCAGCCTGGTCCG
  GGTTCCTGTAGCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGCGCAAGTTCTGTGCTGTCTGCCGCAAG
  GTCCTGGAGGCACCGGCGCTCCACTGCGAAGTGTGTGAGCTGCACCTCCACCCAGACTGTGTGCCCTTCG
  CCTGCAGTGACTGCCGCCAGTGCCACCAGGATGGGCACCAGGATCACGACACCCATCACCACCACTGGCG
  GGAGGGGAACCTGCCCTCGGGAGCGCGCTGCGAGGTCTGCAGGAAGACGTGCGGCTCCTCTGACGTGCTG
  GCCGGCGTGCGCTGCGAGTGGTGCGGGGTCCAGGCGCACTCCCTCTGCTCCGCGGCACTGGCTCCCGAGT
  GTGGCTTCGGGCGTCTGCGCTCCCTGGTCCTGCCTCCCGCGTGCGTGCGCCTTCTGCCCGGCGGCTTCAG
  CAAGACGCAGAGCTTCCGCATCGTGGAGGCCGCGGAGCCGGGCGAGGGGGGCGACGGCGCCGACGGGAGC
  GCTGCCGTGGGTCCAGGCAGAGAGACACAGGCAACTCCGGAGTCCGGGAAGCAAACGCTGAAGATCTTTG
  ATGGCGACGACGCGGTGAGAAGAAGCCAGTTCCGCCTCGTCACGGTGTCCCGCCTGGCCGGTGCCGAGGA
  GGTGCTGGAGGCCGCACTGCGGGCCCACCACATCCCCGAGGACCCTGGCCACCTGGAGCTGTGCCGGCTG
  CCCCCTTCCTCTCAGGCCTGTGACGCCTGGGCTGGGGGCAAGGCTGGGAGTGCTGTGATCTCGGAGGAGG
  GCAGAAGCCCCGGGTCCGGCGAGGCCACGCCAGAGGCCTGGGTCATCCGGGCTCTGCCGCGGGCCCAGGA
  GGTCCTGAAGATCTACCCTGGCTGGCTCAAGGTGGGCGTGGCCTACGTGTCCGTGCGAGTGACCCCGAAG
  AGCACGGCCCGCTCTGTGGTGCTGGAGGTCCTGCCGCTGCTCGGCCGCCAGGCCGAGAGTCCCGAGAGCT
  TCCAGCTGGTGGAGGTGGCGATGGGCTGCAGGCACGTCCAGCGGACGATGCTGATGGACGAACAGCCCCT
  GCTGGACCGGCTACAGGACATCCGGCAGATGTCTGTGCGGCAGGTGAGCCAGACGCGGTTCTACGTGGCA
  GAGAGCAGGGATGTAGCCCCGCACGTCTCCCTGTTTGTTGGCGGCCTGCCTCCCGGCCTGTCTCCCGAGG
  AGTACAGCAGCCTGCTGCATGAGGCCGGGGCTACCAAAGCCACCGTGGTGTCCGTGAGTCACATCTACTC
  CTCCCAAGGCGCGGTAGTGTTGGACGTTGCCTGCTTTGCGGAGGCCGAGCGGCTGTACATGCTGCTGAAG
  GACATGGCTGTGCGGGGCCGGCTGCTCACTGCCCTGGTGCTCCCCGACCTGCTGCACGCGAAGCTGCCCC
  CAGACAGCTGTCCCCTCCTTGTGTTCGTGAACCCCAAGAGTGGAGGCCTCAAGGGCCGAGACCTGCTCTG
  CAGCTTCCGGAAGCTACTGAACCCTCATCAGGTCTTCGACCTGACCAACGGAGGTCCTCTTCCCGGGCTC
  CACCTGTTCTCCCAGGTGCCCTGCTTCCGGGTGCTGGTGTGTGGTGGCGATGGCACTGTGGGCTGGGTGC
  TTGGCGCCCTGGAGGAGACACGGTACCGACTGGCCTGCCCGGAGCCTTCTGTGGCCATCCTGCCCCTGGG
  CACAGGGAATGACCTTGGTCGAGTCCTCCGCTGGGGGGCGGGCTACAGCGGCGAGGACCCGTTCTCCGTA
  CTGCTGTCTGTGGACGAGGCCGACGCCGTGCTCATGGACCGCTGGACCATCCTGCTGGATGCCCACGAGG
  CTGGCAGTGCAGAGAACGACACGGCAGACGCAGAGCCCCCCAAGATCGTGCAGATGAGTAACTACTGTGG
  CATTGGCATCGACGCGGAGCTGAGCCTGGACTTCCACCAGGCACGGGAAGAGGAGCCTGGCAAGTTCACA
  AGCAGGCTGCACAACAAGGGTGTGTACGTGCGGGTGGGGCTGCAGAAGATCAGTCACTCTCGGAGCCTGC
  ACAAGCAGATCCGGCTGCAGGTGGAGCGGCAGGAGGTGGAGCTGCCCAGTATTGAAGGCCTCATCTTCAT
  CAACATCCCCAGCTGGGGCTCGGGGGCCGACCTGTGGGGCTCCGACAGCGACACCAGGTTTGAGAAGCCA
  CGCATGGACGACGGGCTGCTGGAGGTTGTGGGCGTGACGGGCGTCGTGCACATGGGCCAGGTCCAGGGTG
  GGCTGCGCTCCGGAATCCGGATTGCCCAGGGTTCCTACTTCCGAGTCACGCTCCTCAAGGCCACCCCGGT
  GCAGGTGGACGGGGAGCCCTGGGTCCAGGCCCCGGGGCACATGATCATCTCAGCTGCTGGCCCTAAGGTG
  CACATGCTGAGGAAGGCCAAGCAGAAGCCGAGGAGGGCCGGGACCACCAGGGATGCCCGGGCGGATGCTG
  CGCCTGCCCCTGAGAGCGATCCTAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209068 representing NM_001347
  Red=Cloning site Green=Tags(s)
  
  MAAAAEPGARAWLGGGSPRPGSPACSLVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKVTLTK
  PTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPVAHCFGPRGLHKRKFCAVCRK
  VLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVL
  AGVRCEWCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGS
  AAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRL
  PPSSQACDAWAGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPK
  STARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVRQVSQTRFYVA
  ESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLK
  DMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGL
  HLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSV
  LLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFT
  SRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKP
  RMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKV
  HMLRKAKQKPRRAGTTRDARADAAPAPESDPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001347
• ORF Size: 2826 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001347.4
• RefSeq Size: 4653 bp
• RefSeq ORF: 2829 bp
• Locus ID: 1609
• UniProt ID: P52824
• Cytogenetics: 4p16.3
• Domains: RA, DAGKa, DAGKc, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 101 kDa
• Gene Summary: The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]