NDUFAB1 (NM_005003) Human Tagged ORF Clone

CAT#: RC208941

NDUFAB1 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa (NDUFAB1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC208941

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  Western blot validation of overexpression lysate (Cat# LY417576) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208941 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NDUFAB1 protein (Cat# TP308941). The protein was produced from HEK293T cells transfected with NDUFAB1 cDNA clone (Cat# RC208941) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDUFAB1
• Synonyms: ACP; ACP1; FASN2A; SDAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208941 representing NM_005003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCGTGTCCTTTCAGCCTATGTCAGCCGCCTGCCCGCGGCCTTTGCGCCGCTGCCCCGGGTCC
  GGATGCTGGCCGTGGCCCGGCCTCTCAGCACCGCTCTCTGCTCCGCGGGGACCCAGACGAGGCTCGGGAC
  TTTGCAGCCGGCCTTAGTGCTCGCGCAGGTTCCTGGTAGAGTTACACAGTTGTGCCGCCAGTATAGCGAC
  ATGCCTCCTTTGACGTTAGAGGGCATCCAGGACCGTGTTCTTTACGTATTGAAACTCTATGACAAGATTG
  ACCCAGAGAAGCTTTCAGTAAATTCTCATTTTATGAAAGACCTGGGCTTAGACAGTTTGGACCAAGTGGA
  GATTATCATGGCCATGGAAGACGAATTTGGGTTTGAAATTCCTGATATAGATGCTGAAAAGTTAATGTGT
  CCACAAGAAATTGTAGATTACATTGCAGATAAGAAGGATGTATATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208941 representing NM_005003
  Red=Cloning site Green=Tags(s)
  
  MASRVLSAYVSRLPAAFAPLPRVRMLAVARPLSTALCSAGTQTRLGTLQPALVLAQVPGRVTQLCRQYSD
  MPPLTLEGIQDRVLYVLKLYDKIDPEKLSVNSHFMKDLGLDSLDQVEIIMAMEDEFGFEIPDIDAEKLMC
  PQEIVDYIADKKDVYE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005003
• ORF Size: 468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005003.3
• RefSeq Size: 804 bp
• RefSeq ORF: 471 bp
• Locus ID: 4706
• UniProt ID: O14561
• Cytogenetics: 16p12.2
• Domains: pp-binding
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 17.2 kDa
• Gene Summary: Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (PubMed:27626371).[UniProtKB/Swiss-Prot Function]