KPNA2 (NM_002266) Human Tagged ORF Clone

CAT#: RC208803

KPNA2 (Myc-DDK-tagged)-Human karyopherin alpha 2 (RAG cohort 1, importin alpha 1) (KPNA2)

Product Images

Circular map for RC208803

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  Western blot validation of overexpression lysate (Cat# LY419418) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208803 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KPNA2 protein (Cat# TP308803). The protein was produced from HEK293T cells transfected with KPNA2 cDNA clone (Cat# RC208803) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KPNA2
• Synonyms: IPOA1; QIP2; RCH1; SRP1-alpha; SRP1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208803 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCAACGAGAATGCTAATACACCAGCTGCCCGTCTTCACAGATTCAAGAACAAGGGAAAAGACA
  GTACAGAAATGAGGCGTCGCAGAATAGAGGTCAATGTGGAGCTGAGGAAAGCTAAGAAGGATGACCAGAT
  GCTGAAGAGGAGAAATGTAAGCTCATTTCCTGATGATGCTACTTCTCCGCTGCAGGAAAACCGCAACAAC
  CAGGGCACTGTAAATTGGTCTGTTGATGACATTGTCAAAGGCATAAATAGCAGCAATGTGGAAAATCAGC
  TCCAAGCTACTCAAGCTGCCAGGAAACTACTTTCCAGAGAAAAACAGCCCCCCATAGACAACATAATCCG
  GGCTGGTTTGATTCCGAAATTTGTGTCCTTCTTGGGCAGAACTGATTGTAGTCCCATTCAGTTTGAATCT
  GCTTGGGCACTCACTAACATTGCTTCTGGGACATCAGAACAAACCAAGGCTGTGGTAGATGGAGGTGCCA
  TCCCAGCATTCATTTCTCTGTTGGCATCTCCCCATGCTCACATCAGTGAACAAGCTGTCTGGGCTCTAGG
  AAACATTGCAGGTGATGGCTCAGTGTTCCGAGACTTGGTTATTAAGTACGGTGCAGTTGACCCACTGTTG
  GCTCTCCTTGCAGTTCCTGAGATGTCATCTTTAGCATGTGGCTACTTACGTAATCTTACCTGGACACTTT
  CTAATCTTTGCCGCAACAAGAATCCTGCACCCCCGATAGATGCTGTTGAGCAGATTCTTCCTACCTTAGT
  TCGGCTCCTGCATCATGATGATCCAGAAGTGTTAGCAGATACCTGCTGGGCTATTTCCTACCTTACTGAT
  GGTCCAAATGAACGAATTGGCATGGTGGTGAAAACAGGAGTTGTGCCCCAACTTGTGAAGCTTCTAGGAG
  CTTCTGAATTGCCAATTGTGACTCCTGCCCTAAGAGCCATAGGGAATATTGTCACTGGTACAGATGAACA
  GACTCAGGTTGTGATTGATGCAGGAGCACTCGCCGTCTTTCCCAGCCTGCTCACCAACCCCAAAACTAAC
  ATTCAGAAGGAAGCTACGTGGACAATGTCAAACATCACAGCCGGCCGCCAGGACCAGATACAGCAAGTTG
  TGAATCATGGATTAGTCCCATTCCTTGTCAGTGTTCTCTCTAAGGCAGATTTTAAGACACAAAAGGAAGC
  TGTGTGGGCCGTGACCAACTATACCAGTGGTGGAACAGTTGAACAGATTGTGTACCTTGTTCACTGTGGC
  ATAATAGAACCGTTGATGAACCTCTTAACTGCAAAAGATACCAAGATTATTCTGGTTATCCTGGATGCCA
  TTTCAAATATCTTTCAGGCTGCTGAGAAACTAGGTGAAACTGAGAAACTTAGTATAATGATTGAAGAATG
  TGGAGGCTTAGACAAAATTGAAGCTCTACAAAACCATGAAAATGAGTCTGTGTATAAGGCTTCGTTAAGC
  TTAATTGAGAAGTATTTCTCTGTAGAGGAAGAGGAAGATCAAAACGTTGTACCAGAAACTACCTCTGAAG
  GCTACACTTTCCAAGTTCAGGATGGGGCTCCTGGGACCTTTAACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208803 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNN
  QGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFES
  AWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLL
  ALLAVPEMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTD
  GPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTN
  IQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCG
  IIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLS
  LIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002266
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002266.4
• RefSeq Size: 2011 bp
• RefSeq ORF: 1590 bp
• Locus ID: 3838
• UniProt ID: P52292
• Cytogenetics: 17q24.2
• Domains: Armadillo_seg, IBB
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 57.9 kDa
• Gene Summary: The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]