UNG (NM_003362) Human Tagged ORF Clone

CAT#: RC208667

UNG (Myc-DDK-tagged)-Human uracil-DNA glycosylase (UNG), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC208667

• 

  Western blot validation of overexpression lysate (Cat# LY418736) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208667 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UNG
• Synonyms: DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208667 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTCTTCTGCCTTGGGCCGTGGGGGTTGGGCCGGAAGCTGCGGACGCCTGGGAAGGGGCCGCTGC
  AGCTCTTGAGCCGCCTCTGCGGGGACCACTTGCAGGCCATCCCAGCCAAGAAGGCCCCGGCTGGGCAGGA
  GGAGCCTGGGACGCCGCCCTCCTCGCCGCTGAGTGCCGAGCAGTTGGACCGGATCCAGAGGAACAAGGCC
  GCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTGGGCTTTGGAGAGAGCTGGAAGAAGCACCTCA
  GCGGGGAGTTCGGGAAACCGTATTTTATCAAGCTAATGGGATTTGTTGCAGAAGAAAGAAAGCATTACAC
  TGTTTATCCACCCCCACACCAAGTCTTCACCTGGACCCAGATGTGTGACATAAAAGATGTGAAGGTTGTC
  ATCCTGGGACAGGATCCATATCATGGACCTAATCAAGCTCACGGGCTCTGCTTTAGTGTTCAAAGGCCTG
  TTCCGCCTCCGCCCAGTTTGGAGAACATTTATAAAGAGTTGTCTACAGACATAGAGGATTTTGTTCATCC
  TGGCCATGGAGATTTATCTGGGTGGGCCAAGCAAGGTGTTCTCCTTCTCAACGCTGTCCTCACGGTTCGT
  GCCCATCAAGCCAACTCTCATAAGGAGCGAGGCTGGGAGCAGTTCACTGATGCAGTTGTGTCCTGGCTAA
  ATCAGAACTCGAATGGCCTTGTTTTCTTGCTCTGGGGCTCTTATGCTCAGAAGAAGGGCAGTGCCATTGA
  TAGGAAGCGGCACCATGTACTACAGACGGCTCATCCCTCCCCTTTGTCAGTGTATAGAGGGTTCTTTGGA
  TGTAGACACTTTTCAAAGACCAATGAGCTGCTGCAGAAGTCTGGCAAGAAGCCCATTGACTGGAAGGAGC
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208667 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKA
  AALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVV
  ILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVR
  AHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFG
  CRHFSKTNELLQKSGKKPIDWKEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003362
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003362.4
• RefSeq Size: 2166 bp
• RefSeq ORF: 915 bp
• Locus ID: 7374
• UniProt ID: P13051
• Cytogenetics: 12q24.11
• Domains: UDG
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Primary immunodeficiency
• MW: 33.9 kDa
• Gene Summary: This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]