CD31 (PECAM1) (NM_000442) Human Tagged ORF Clone

CAT#: RC208654

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PECAM1 (Myc-DDK-tagged)-Human platelet/endothelial cell adhesion molecule (PECAM1)



  "NM_000442" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PECAM1
Synonyms CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208654 representing NM_000442
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCGAGGTGGGCCCAAGGGGCCACGATGTGGCTTGGAGTCCTGCTGACCCTTCTGCTCTGTTCAA
GCCTTGAGGGTCAAGAAAACTCTTTCACAATCAACAGTGTTGACATGAAGAGCCTGCCGGACTGGACGGT
GCAAAATGGGAAGAACCTGACCCTGCAGTGCTTCGCGGATGTCAGCACCACCTCTCACGTCAAGCCTCAG
CACCAGATGCTGTTCTATAAGGATGACGTGCTGTTTTACAACATCTCCTCCATGAAGAGCACAGAGAGTT
ATTTTATTCCTGAAGTCCGGATCTATGACTCAGGGACATATAAATGTACTGTGATTGTGAACAACAAAGA
GAAAACCACTGCAGAGTACCAGGTGTTGGTGGAAGGAGTGCCCAGTCCCAGGGTGACACTGGACAAGAAA
GAGGCCATCCAAGGTGGGATCGTGAGGGTCAACTGTTCTGTCCCAGAGGAAAAGGCCCCAATACACTTCA
CAATTGAAAAACTTGAACTAAATGAAAAAATGGTCAAGCTGAAAAGAGAGAAGAATTCTCGAGACCAGAA
TTTTGTGATACTGGAATTCCCCGTTGAGGAACAGGACCGCGTTTTATCCTTCCGATGTCAAGCTAGGATC
ATTTCTGGGATCCATATGCAGACCTCAGAATCTACCAAGAGTGAACTGGTCACCGTGACGGAATCCTTCT
CTACACCCAAGTTCCACATCAGCCCCACCGGAATGATCATGGAAGGAGCTCAGCTCCACATTAAGTGCAC
CATTCAAGTGACTCACCTGGCCCAGGAGTTTCCAGAAATCATAATTCAGAAGGACAAGGCGATTGTGGCC
CACAACAGACATGGCAACAAGGCTGTGTACTCAGTCATGGCCATGGTGGAGCACAGTGGCAACTACACGT
GCAAAGTGGAGTCCAGCCGCATATCCAAGGTCAGCAGCATCGTGGTCAACATAACAGAACTATTTTCCAA
GCCCGAACTGGAATCTTCCTTCACACATCTGGACCAAGGTGAAAGACTGAACCTGTCCTGCTCCATCCCA
GGAGCACCTCCAGCCAACTTCACCATCCAGAAGGAAGATACGATTGTGTCACAGACTCAAGATTTCACCA
AGATAGCCTCAAAGTCGGACAGTGGGACGTATATCTGCACTGCAGGTATTGACAAAGTGGTCAAGAAAAG
CAACACAGTCCAGATAGTCGTATGTGAAATGCTCTCCCAGCCCAGGATTTCTTATGATGCCCAGTTTGAG
GTCATAAAAGGACAGACCATCGAAGTCCGTTGCGAATCGATCAGTGGAACTTTGCCTATTTCTTACCAAC
TTTTAAAAACAAGTAAAGTTTTGGAGAATAGTACCAAGAACTCAAATGATCCTGCGGTATTCAAAGACAA
CCCCACTGAAGACGTCGAATACCAGTGTGTTGCAGATAATTGCCATTCCCACGCCAAAATGTTAAGTGAG
GTTCTGAGGGTGAAGGTGATAGCCCCGGTGGATGAGGTCCAGATTTCTATCCTGTCAAGTAAGGTGGTGG
AGTCTGGAGAGGACATTGTGCTGCAATGTGCTGTGAATGAAGGATCTGGTCCCATCACCTATAAGTTTTA
CAGAGAAAAAGAGGGCAAACCCTTCTATCAAATGACCTCAAATGCCACCCAGGCATTTTGGACCAAGCAG
AAGGCTAACAAGGAACAGGAGGGAGAGTATTACTGCACAGCCTTCAACAGAGCCAACCACGCCTCCAGTG
TCCCCAGAAGCAAAATACTGACAGTCAGAGTCATTCTTGCCCCATGGAAGAAAGGACTTATTGCAGTGGT
TATCATCGGAGTGATCATTGCTCTCTTGATCATTGCGGCCAAATGTTATTTTCTGAGGAAAGCCAAGGCC
AAGCAGATGCCAGTGGAAATGTCCAGGCCAGCAGTACCACTTCTGAACTCCAACAACGAGAAAATGTCAG
ATCCCAATATGGAAGCTAACAGTCATTACGGTCACAATGACGATGTCGGAAACCATGCAATGAAACCAAT
AAATGATAATAAAGAGCCTCTGAACTCAGACGTGCAGTACACGGAAGTTCAAGTGTCCTCAGCTGAGTCT
CACAAAGATCTAGGAAAGAAGGACACAGAGACAGTGTACAGTGAAGTCCGGAAAGCTGTCCCTGATGCCG
TGGAAAGCAGATACTCTAGAACGGAAGGCTCCCTTGATGGAACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208654 representing NM_000442
Red=Cloning site Green=Tags(s)

MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQ
HQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKK
EAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARI
ISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVA
HNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIP
GAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFE
VIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSE
VLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQ
KANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKA
KQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAES
HKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000442
ORF Size 2214 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000442.2, NP_000433.2
RefSeq Size 3754 bp
RefSeq ORF 2217 bp
Locus ID 5175
UniProt ID P16284
Cytogenetics 17q23.3
Domains ig, IGc2, IG
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
MW 82.4 kDa
Gene Summary The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

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