ATP1A2 (NM_000702) Human Tagged ORF Clone

CAT#: RC208606

ATP1A2 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, alpha 2 polypeptide (ATP1A2)

Product Images

Western blot validation of overexpression lysate (Cat# LY424562) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208606 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP1A2
• Synonyms: FHM2; MHP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208606 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGTGGGGCTGGCCGTGAGTACTCACCTGCCGCCACCACGGCAGAGAATGGGGGCGGCAAGAAGA
  AACAGAAGGAGAAGGAACTGGATGAGCTGAAGAAGGAGGTGGCAATGGATGACCACAAGCTGTCCTTGGA
  TGAGCTGGGCCGCAAATACCAAGTGGACCTGTCCAAGGGCCTCACCAACCAGCGGGCTCAGGACGTTCTG
  GCTCGAGATGGGCCCAACGCCCTCACACCACCTCCCACAACCCCTGAGTGGGTCAAGTTCTGCCGTCAGC
  TTTTCGGGGGGTTCTCCATCCTGCTGTGGATTGGGGCTATCCTCTGCTTCCTGGCCTACGGCATCCAGGC
  TGCCATGGAGGATGAACCATCCAACGACAATCTATATCTGGGTGTGGTGCTGGCAGCTGTGGTCATTGTC
  ACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGATCATGGATTCCTTCAAGAACATGGTAC
  CTCAGCAAGCCCTTGTGATCCGGGAGGGAGAGAAGATGCAGATCAACGCAGAGGAAGTGGTGGTGGGAGA
  CCTGGTGGAGGTGAAGGGTGGAGACCGCGTCCCTGCTGACCTCCGGATCATCTCTTCTCATGGCTGTAAG
  GTGGATAACTCATCCTTAACAGGAGAGTCGGAGCCCCAGACCCGCTCCCCCGAGTTCACCCATGAGAACC
  CCCTGGAGACCCGCAATATCTGTTTCTTCTCCACCAACTGTGTTGAAGGCACTGCCAGGGGCATTGTGAT
  TGCCACAGGAGACCGGACGGTGATGGGCCGCATAGCTACTCTCGCCTCAGGCCTGGAGGTTGGGCGGACA
  CCCATAGCAATGGAGATTGAACACTTCATCCAGCTGATCACAGGGGTCGCTGTATTCCTGGGGGTCTCCT
  TCTTCGTGCTCTCCCTCATCCTGGGCTACAGCTGGCTGGAGGCAGTCATCTTCCTCATCGGCATCATAGT
  GGCCAACGTGCCTGAGGGGCTTCTGGCCACTGTCACTGTGTGCCTGACCCTGACAGCCAAGCGCATGGCA
  CGGAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGG
  ACAAGACGGGCACCCTCACCCAGAACCGCATGACCGTCGCCCACATGTGGTTCGACAACCAAATCCATGA
  GGCTGACACCACCGAAGATCAGTCTGGGGCCACTTTTGACAAACGATCCCCTACGTGGACGGCCCTGTCT
  CGAATTGCTGGTCTCTGCAACCGCGCCGTCTTCAAGGCAGGACAGGAGAACATCTCCGTGTCTAAGCGGG
  ACACAGCTGGTGATGCCTCTGAGTCAGCTCTGCTCAAGTGCATTGAGCTCTCCTGTGGCTCAGTGAGGAA
  AATGAGAGACAGAAACCCCAAGGTGGCAGAGATTCCTTTCAACTCTACCAACAAGTACCAGCTGTCTATC
  CACGAGCGAGAAGACAGCCCCCAGAGCCACGTGCTGGTGATGAAGGGGGCCCCAGAGCGCATTCTGGACC
  GGTGCTCCACCATCCTGGTGCAGGGCAAGGAGATCCCGCTCGACAAGGAGATGCAAGATGCCTTTCAAAA
  TGCCTACATGGAGCTGGGGGGACTTGGGGAGCGTGTGCTGGGATTCTGTCAACTGAATCTGCCATCTGGA
  AAGTTTCCTCGGGGCTTCAAATTCGACACGGATGAGCTGAACTTTCCCACGGAGAAGCTTTGCTTTGTGG
  GGCTCATGTCTATGATTGACCCTCCCCGGGCTGCTGTGCCAGATGCTGTGGGCAAGTGCCGAAGCGCAGG
  CATCAAGGTGATCATGGTAACCGGGGATCACCCTATCACAGCCAAGGCCATTGCCAAAGGCGTGGGCATC
  ATATCAGAGGGTAACGAGACTGTGGAGGACATTGCAGCCCGGCTCAACATTCCCATGAGTCAAGTCAACC
  CCAGAGAAGCCAAGGCATGCGTGGTGCACGGCTCTGACCTGAAGGACATGACATCGGAGCAGCTCGATGA
  GATCCTCAAGAACCACACAGAGATCGTCTTTGCTCGAACGTCTCCCCAGCAGAAGCTCATCATTGTGGAG
  GGATGTCAGAGGCAGGGAGCCATTGTGGCCGTGACGGGTGACGGGGTGAACGACTCCCCTGCATTGAAGA
  AGGCTGACATTGGCATTGCCATGGGCATCTCTGGCTCTGACGTCTCTAAGCAGGCAGCCGACATGATCCT
  GCTGGATGACAACTTTGCCTCCATCGTCACGGGGGTGGAGGAGGGCCGCCTGATCTTTGACAACTTGAAG
  AAATCCATCGCCTACACCCTGACCAGCAACATCCCCGAGATCACCCCCTTCCTGCTGTTCATCATTGCCA
  ACATCCCCCTACCTCTGGGCACTGTGACCATCCTTTGCATTGACCTGGGCACAGATATGGTCCCTGCCAT
  CTCCTTGGCCTATGAGGCAGCTGAGAGTGATATCATGAAGCGGCAGCCACGAAACTCCCAGACGGACAAG
  CTGGTGAATGAGAGGCTCATCAGCATGGCCTACGGACAGATCGGGATGATCCAGGCACTGGGTGGCTTCT
  TCACCTACTTTGTGATCCTGGCAGAGAACGGTTTCCTGCCATCACGGCTACTGGGAATCCGCCTCGACTG
  GGATGACCGGACCATGAATGATCTGGAGGACAGCTATGGACAGGAGTGGACCTATGAGCAGCGGAAGGTG
  GTGGAGTTCACGTGCCACACGGCATTCTTTGCCAGCATCGTGGTGGTGCAGTGGGCTGACCTCATCATCT
  GCAAGACCCGCCGCAACTCAGTCTTCCAGCAGGGCATGAAGAACAAGATCCTGATTTTTGGGCTCCTGGA
  GGAGACGGCGTTGGCTGCCTTTCTCTCTTACTGCCCAGGCATGGGTGTAGCCCTCCGCATGTACCCGCTC
  AAAGTCACCTGGTGGTTCTGCGCCTTCCCCTACAGCCTCCTCATCTTCATCTATGATGAGGTCCGAAAGC
  TCATCCTGCGGCGGTATCCTGGTGGCTGGGTGGAGAAGGAGACATACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208606 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRGAGREYSPAATTAENGGGKKKQKEKELDELKKEVAMDDHKLSLDELGRKYQVDLSKGLTNQRAQDVL
  ARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAAMEDEPSNDNLYLGVVLAAVVIV
  TGCFSYYQEAKSSKIMDSFKNMVPQQALVIREGEKMQINAEEVVVGDLVEVKGGDRVPADLRIISSHGCK
  VDNSSLTGESEPQTRSPEFTHENPLETRNICFFSTNCVEGTARGIVIATGDRTVMGRIATLASGLEVGRT
  PIAMEIEHFIQLITGVAVFLGVSFFVLSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMA
  RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALS
  RIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSI
  HEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSG
  KFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGI
  ISEGNETVEDIAARLNIPMSQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVE
  GCQRQGAIVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLK
  KSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIMKRQPRNSQTDK
  LVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPSRLLGIRLDWDDRTMNDLEDSYGQEWTYEQRKV
  VEFTCHTAFFASIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLLEETALAAFLSYCPGMGVALRMYPL
  KVTWWFCAFPYSLLIFIYDEVRKLILRRYPGGWVEKETYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000702
• ORF Size: 3060 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000702.4
• RefSeq Size: 5496 bp
• RefSeq ORF: 3063 bp
• Locus ID: 477
• UniProt ID: P50993
• Cytogenetics: 1q23.2
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction
• MW: 112.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]