XYLT2 (NM_022167) Human Tagged ORF Clone

CAT#: RC208572

XYLT2 (Myc-DDK-tagged)-Human xylosyltransferase II (XYLT2)

Product Images

Circular map for RC208572

• 

  Western blot validation of overexpression lysate (Cat# LY411722) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208572 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XYLT2
• Synonyms: PXYLT2; SOS; XT-II; XT2; xylT-II
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208572 representing NM_022167
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACGGCGCTGGCCA
  TCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAGGACGAGGCGGGCGAGAAAGG
  AAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGTTCCAAGGACACAGACAGTTCAGCAGGGCGA
  CGGGGCAGCACAGGCAGAAGGCATGGGCGCTGGCGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCA
  AGGTGGTACGGGCAGTAACCAGCCGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGC
  CCCAGGCCGCCAGAACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCA
  CACGGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCCAAGTGCGAGA
  TCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAGTGCCAGCAGGAGATCGCCAA
  TGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAGGCTGTGCCCCGGCACTGTCAGCTGACTGGG
  AAGATGAGCCCCGGCATCCAGTGGGATGAGAGCCAAGCCCAGCAGCCCATGGATGGCCCCCCGGTGCGAA
  TCGCCTACATGCTGGTGGTTCACGGCCGCGCCATCCGCCAGCTGAAGCGTCTCCTCAAGGCCGTTTATCA
  CGAGCAGCACTTCTTTTACATCCATGTGGACAAGCGTTCCGACTACCTGCACCGGGAGGTGGTGGAGCTG
  GCCCAGGGCTATGATAACGTGCGGGTGACGCCCTGGCGCATGGTTACCATCTGGGGCGGGGCCAGCCTCC
  TGACGATGTACCTGCGGAGCATGCGGGACCTGCTAGAGGTGCCTGGCTGGGCCTGGGACTTCTTCATCAA
  CCTCAGTGCCACTGACTATCCAACCAGGACCAATGAGGAGCTGGTGGCATTCCTATCCAAGAACCGGGAC
  AAGAATTTCCTCAAGTCACATGGCCGGGACAACTCCAGGTTCATCAAGAAACAGGGCCTGGACCGGCTCT
  TCCATGAGTGCGACTCACACATGTGGCGCCTGGGCGAGCGGCAGATCCCAGCAGGCATTGTGGTGGATGG
  CGGTTCTGACTGGTTCGTGCTGACACGCAGCTTTGTGGAGTATGTGGTGTACACAGATGACCCGCTTGTG
  GCCCAGCTGCGCCAGTTCTACACATACACACTGCTCCCAGCCGAGTCCTTCTTCCACACGGTGCTGGAGA
  ACAGCCTGGCCTGTGAGACCCTCGTGGACAACAACCTGCGGGTCACCAACTGGAACCGCAAGCTGGGCTG
  CAAGTGCCAGTACAAGCACATTGTGGACTGGTGTGGCTGCTCCCCCAACGACTTCAAGCCACAGGACTTC
  CTCCGGCTGCAGCAAGTCTCCAGACCCACCTTCTTCGCCCGGAAGTTCGAGTCGACTGTGAACCAGGAGG
  TGCTGGAAATCCTGGACTTCCACCTGTACGGCAGCTACCCCCCCGGCACGCCAGCCCTCAAGGCCTACTG
  GGAGAACACCTACGACGCGGCTGATGGCCCCAGTGGGCTCAGTGATGTCATGCTCACTGCTTACACAGCC
  TTCGCCCGCCTCAGCCTGCACCATGCCGCCACTGCTGCACCCCCAATGGGCACCCCACTCTGCAGGTTTG
  AGCCCAGGGGCTTGCCGTCCAGCGTGCACCTGTATTTCTATGACGACCATTTCCAGGGCTACCTGGTGAC
  GCAGGCGGTGCAGCCCTCAGCCCAGGGGCCGGCAGAGACGCTTGAGATGTGGCTGATGCCCCAAGGGTCG
  CTGAAGCTGTTGGGGCGCAGTGACCAGGCCAGCCGGCTCCAGAGTCTGGAGGTTGGCACTGATTGGGACC
  CCAAAGAGCGTCTTTTCCGGAACTTTGGGGGGTTACTGGGGCCGCTGGACGAGCCTGTGGCCGTGCAGCG
  CTGGGCCCGGGGCCCCAACCTCACAGCCACAGTGGTCTGGATCGACCCAACCTATGTGGTGGCCACATCT
  TATGACATCACAGTAGATACGGAGACTGAGGTCACGCAATACAAGCCCCCACTGAGCCGGCCCCTGCGGC
  CAGGGCCCTGGACTGTTCGACTCCTTCAGTTCTGGGAACCGCTGGGTGAGACCCGCTTCCTTGTGCTGCC
  CTTGACCTTCAACCGCAAACTACCTCTCAGGAAAGATGATGCCAGCTGGCTGCACGCAGGGCCACCCCAC
  AACGAGTACATGGAGCAGAGTTTCCAGGGCCTGAGTAGCATCCTGAACCTGCCTCAGCCGGAGCTCGCGG
  AGGAGGCTGCCCAGCGGCACACACAGCTCACAGGCCCTGCGCTCGAGGCCTGGACAGACAGGGAACTGAG
  CAGCTTCTGGTCCGTGGCTGGACTGTGTGCCATAGGCCCCTCTCCCTGCCCCTCCCTGGAGCCCTGCAGA
  CTGACCAGCTGGAGCTCTCTGTCCCCCGACCCCAAATCAGAGCTGGGGCCTGTCAAAGCAGACGGGCGAC
  TCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208572 representing NM_022167
  Red=Cloning site Green=Tags(s)
  
  MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEAGEKGRQRKPRPLDPGEGSKDTDSSAGR
  RGSTGRRHGRWRGRAESPGVPVAKVVRAVTSRQRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPP
  HGDTGSVEGAPQPTDNGFTPKCEIVGKDALSALARASTKQCQQEIANVVCLHQAGSLMPKAVPRHCQLTG
  KMSPGIQWDESQAQQPMDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSDYLHREVVEL
  AQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRD
  KNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAGIVVDGGSDWFVLTRSFVEYVVYTDDPLV
  AQLRQFYTYTLLPAESFFHTVLENSLACETLVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDF
  LRLQQVSRPTFFARKFESTVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDAADGPSGLSDVMLTAYTA
  FARLSLHHAATAAPPMGTPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMWLMPQGS
  LKLLGRSDQASRLQSLEVGTDWDPKERLFRNFGGLLGPLDEPVAVQRWARGPNLTATVVWIDPTYVVATS
  YDITVDTETEVTQYKPPLSRPLRPGPWTVRLLQFWEPLGETRFLVLPLTFNRKLPLRKDDASWLHAGPPH
  NEYMEQSFQGLSSILNLPQPELAEEAAQRHTQLTGPALEAWTDRELSSFWSVAGLCAIGPSPCPSLEPCR
  LTSWSSLSPDPKSELGPVKADGRLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022167
• ORF Size: 2595 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022167.1, NP_071450.1
• RefSeq Size: 3567 bp
• RefSeq ORF: 2598 bp
• Locus ID: 64132
• UniProt ID: Q9H1B5
• Cytogenetics: 17q21.33
• Domains: Branch
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• MW: 96.6 kDa
• Gene Summary: The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]