XYLT2 (NM_022167) Human Untagged Clone

CAT#: SC112785

XYLT2 (untagged)-Human xylosyltransferase II (XYLT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XYLT2
• Synonyms: PXYLT2; SOS; XT-II; XT2; xylT-II
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112785 sequence for NM_022167 edited (data generated by NextGen Sequencing)
  ATGGTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACG
  GCGCTGGCCATCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAG
  GACGAGGCGGGCGAGAAAGGAAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGT
  TCCAAGGACACAGACAGTTCAGCAGGGCGACGGGGCAGCACAGGCAGAAGGCATGGGCGC
  TGGCGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCAAGGTGGTACGGGCAGTAACC
  AGCCGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGCCCCAGGCCGC
  CAGAACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCA
  CACGGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCC
  AAGTGCGAGATCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAG
  TGCCAGCAGGAGATCGCCAATGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAG
  GCTGTGCCCCGGCACTGTCAGCTGACTGGGAAGATGAGCCCCGGCATCCAGTGGGATGAG
  AGCCAAGCCCAGCAGCCCATGGATGGCCCCCCGGTGCGAATCGCCTACATGCTGGTGGTT
  CACGGCCGCGCCATCCGCCAGCTGAAGCGTCTCCTCAAGGCCGTTTATCACGAGCAGCAC
  TTCTTTTACATCCATGTGGACAAGCGTTCCGACTACCTGCACCGGGAGGTGGTGGAGCTG
  GCCCAGGGCTATGATAACGTGCGGGTGACGCCCTGGCGCATGGTTACCATCTGGGGCGGG
  GCCAGCCTCCTGACGATGTACCTGCGGAGCATGCGGGACCTGCTAGAGGTGCCTGGCTGG
  GCCTGGGACTTCTTCATCAACCTCAGTGCCACTGACTATCCAACCAGGACCAATGAGGAG
  CTGGTGGCATTCCTATCCAAGAACCGGGACAAGAATTTCCTCAAGTCACATGGCCGGGAC
  AACTCCAGGTTCATCAAGAAACAGGGCCTGGACCGGCTCTTCCATGAGTGCGACTCACAC
  ATGTGGCGCCTGGGCGAGCGGCAGATCCCAGCAGGCATTGTGGTGGATGGCGGTTCTGAC
  TGGTTCGTGCTGACACGCAGCTTTGTGGAGTATGTGGTGTACACAGATGACCCGCTTGTG
  GCCCAGCTGCGCCAGTTCTACACATACACACTGCTCCCAGCCGAGTCCTTCTTCCACACG
  GTGCTGGAGAACAGCCTGGCCTGTGAGACCCTCGTGGACAACAACCTGCGGGTCACCAAC
  TGGAACCGCAAGCTGGGCTGCAAGTGCCAGTACAAGCACATTGTGGACTGGTGTGGCTGC
  TCCCCCAACGACTTCAAGCCACAGGACTTCCTCCGGCTGCAGCAAGTCTCCAGACCCACC
  TTCTTCGCCCGGAAGTTCGAGTCGACTGTGAACCAGGAGGTGCTGGAAATCCTGGACTTC
  CACCTGTACGGCAGCTACCCCCCCGGCACGCCAGCCCTCAAGGCCTACTGGGAGAACACC
  TACGACGCGGCTGATGGCCCCAGTGGGCTCAGTGATGTCATGCTCACTGCTTACACAGCC
  TTCGCCCGCCTCAGCCTGCACCATGCCGCCACTGCTGCACCCCCAATGGGCACCCCACTC
  TGCAGGTTTGAGCCCAGGGGCTTGCCGTCCAGCGTGCACCTGTATTTCTATGACGACCAT
  TTCCAGGGCTACCTGGTGACGCAGGCGGTGCAGCCCTCAGCCCAGGGGCCGGCAGAGACG
  CTTGAGATGTGGCTGATGCCCCAAGGGTCGCTGAAGCTGTTGGGGCGCAGTGACCAGGCC
  AGCCGGCTCCAGAGTCTGGAGGTTGGCACTGATTGGGACCCCAAAGAGCGTCTTTTCCGG
  AACTTTGGGGGGTTACTGGGGCCGCTGGACGAGCCTGTGGCCGTGCAGCGCTGGGCCCGG
  GGCCCCAACCTCACAGCCACAGTGGTCTGGATCGACCCAACCTATGTGGTGGCCACATCT
  TATGACATCACAGTAGATACGGAGACTGAGGTCACGCAATACAAGCCCCCACTGAGCCGG
  CCCCTGCGGCCAGGGCCCTGGACTGTTCGACTCCTTCAGTTCTGGGAACCGCTGGGTGAG
  ACCCGCTTCCTTGTGCTGCCCTTGACCTTCAACCGCAAACTACCTCTCAGGAAAGATGAT
  GCCAGCTGGCTGCACGCAGGGCCACCCCACAACGAGTACATGGAGCAGAGTTTCCAGGGC
  CTGAGTAGCATCCTGAACCTGCCTCAGCCGGAGCTCGCGGAGGAGGCTGCCCAGCGGCAC
  ACACAGCTCACAGGCCCTGCGCTCGAGGCCTGGACAGACAGGGAACTGAGCAGCTTCTGG
  TCCGTGGCTGGACTGTGTGCCATAGGCCCCTCTCCCTGCCCCTCCCTGGAGCCCTGCAGA
  CTGACCAGCTGGAGCTCTCTGTCCCCCGACCCCAAATCAGAGCTGGGGCCTGTCAAAGCA
  GACGGGCGACTCAGGTAG
  
  Clone variation with respect to NM_022167.2
  177 g=>a;342 c=>t;914 g=>c;1569 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022167 unedited
  TATAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGTTGCAGGGCTGGGCGCGCGCCCCGCGTCCCGGGCAGGAAGATG
  GTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACGGCG
  CTGGCCATCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAGGAC
  GAGGCGGGCGAGAAAGGAAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGTTCC
  AAGGACACAGACAGTTCAGCAGGGCGACGGGGCAGCACAGGCAGAAGGCATGGGCGCTGG
  CGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCAAGGTGGTACGGGCAGTAACCAGC
  CGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGCCCCAGGCCGCCAG
  AACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCACAC
  GGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCCAAG
  TGCGAGATCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAGTGC
  CAGCAGGAGATCGCCAATGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAGGCT
  GTGCCCCGGCACTGTCAGCTGACTGGGAAGATGAGCCCCGGCATCCAGTGGGATGAGAGC
  CAAGCCCAGCAGCCCATGGATGGCCCCNCGGTGCGAATCGNCTCATGCTGGTGGTTCACG
  GCCGCGCCATCCGCCAGCTGGAGCGTCTNCTCAAGGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022167 unedited
  CGGGGAAATACTATGTACGCGGCCGCTTTCTANGATCGATTTTTTTTTTTTTTTTTTAGA
  GGAGAAGAGATATATTTCTTCTGTTGCATATTTCAGACTTCACGCTGAGCTTCAGGCACA
  ATTACTCCAGGTTCTTCTGAGAGTCTTGACAGAGTCTTCTTCCCACGCTGCTCATGTGAC
  CTCACTTCCAGTGCACACCTGGAAACTCTGCATGGGTCTCATTGGGCCCTGACCCTGCCT
  TTCCCATCCAGGGGCAGGGTATGCTGGAAAGCCCTGGAGCCGCCCCACCCCACTTCTAGG
  GTCCCCTGGCTACAAAAGAACTGTCCTGCTAGAGCCCAGCTCTGCAGCGCCCCCGATATC
  ACCTGTTCTTCGGGCCTTAGAGCCTGGCAGGGCACTTGCAGCCCTGTGGAGCCTCAGCCA
  CGAGCACCACAGGCCCAGAATGGGGCATGAGGCCCTGGAGAGTGAGGCAAGCCCAGCCTC
  GTGCAGAACAATAGCAGGTGGGCTCAACAAGCCCCTGCTCCCCACAGACATGGCCCACTT
  CCTGGGGCTGCTCTGAGCCCTATTGCACATCTTCTGGTCTAGTTTCTCTCCCAACCACCC
  ATTTTCCTTTTTAAATTTAAAACAAAAAAAGAAAAAGAAAAAAAAGAAATTCCCACTATA
  CCGGAAGGTGAAGAAGAACTGGGGGTGGAATGCTGCTGTGCCCCTTTTGGGTTTCCGCCA
  GCCCTCTGGATCCCCACAAAAAACTCAGCAGTAGTTTATAATGGGGCCACCTTTCCTGGC
  CCCTGTGTGGGGTTCTTGAAGGGTCAAAAGGGGGGGCAACCCTGGGCCCCTGGTTTCTTG
  CCCGGTGGGAGAGGAGAACCCCTCCCCTGGGTTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022167
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022167.1, NP_071450.1
• RefSeq Size: 3568 bp
• RefSeq ORF: 2598 bp
• Locus ID: 64132
• UniProt ID: Q9H1B5
• Cytogenetics: 17q21.33
• Domains: Branch
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (1) represents the protein-coding transcript.