APOBEC2 (NM_006789) Human Tagged ORF Clone

CAT#: RC208399

APOBEC2 (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2 (APOBEC2)

Product Images

Circular map for RC208399

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  Western blot validation of overexpression lysate (Cat# LY416413) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208399 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified APOBEC2 protein (Cat# TP308399). The protein was produced from HEK293T cells transfected with APOBEC2 cDNA clone (Cat# RC208399) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APOBEC2
• Synonyms: ARCD1; ARP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208399 representing NM_006789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGAAGGAAGAGGCTGCTGTGGCCACTGAGGCTGCCTCCCAGAATGGGGAGGATCTGGAGAACC
  TGGACGACCCTGAGAAGCTGAAAGAGCTGATTGAGCTGCCGCCCTTTGAGATTGTCACAGGAGAACGGCT
  GCCTGCCAACTTCTTTAAATTCCAGTTCCGGAATGTGGAGTACAGTTCCGGGAGGAACAAGACCTTCCTC
  TGCTATGTGGTTGAAGCACAGGGCAAGGGGGGCCAAGTGCAGGCATCTCGGGGATACCTAGAGGATGAGC
  ATGCGGCTGCCCATGCAGAGGAAGCTTTCTTCAACACCATCCTGCCAGCCTTCGACCCAGCCCTGCGGTA
  CAATGTCACCTGGTATGTGTCCTCCAGCCCCTGTGCAGCGTGTGCTGACCGCATTATCAAAACCCTTAGC
  AAGACCAAGAACCTGCGTCTGCTCATTCTGGTGGGTCGACTCTTCATGTGGGAGGAGCCGGAGATCCAGG
  CTGCTCTGAAGAAGCTGAAGGAGGCTGGCTGTAAACTGCGCATCATGAAGCCCCAGGACTTCGAATATGT
  CTGGCAGAATTTTGTGGAGCAAGAAGAGGGTGAATCCAAGGCCTTTCAGCCCTGGGAGGACATTCAGGAG
  AACTTCCTATACTACGAGGAGAAGTTGGCAGACATCCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208399 representing NM_006789
  Red=Cloning site Green=Tags(s)
  
  MAQKEEAAVATEAASQNGEDLENLDDPEKLKELIELPPFEIVTGERLPANFFKFQFRNVEYSSGRNKTFL
  CYVVEAQGKGGQVQASRGYLEDEHAAAHAEEAFFNTILPAFDPALRYNVTWYVSSSPCAACADRIIKTLS
  KTKNLRLLILVGRLFMWEEPEIQAALKKLKEAGCKLRIMKPQDFEYVWQNFVEQEEGESKAFQPWEDIQE
  NFLYYEEKLADILK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006789
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006789.3, NP_006780.1
• RefSeq Size: 1195 bp
• RefSeq ORF: 675 bp
• Locus ID: 10930
• UniProt ID: Q9Y235
• Cytogenetics: 6p21.1
• MW: 25.5 kDa
• Gene Summary: Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.[UniProtKB/Swiss-Prot Function]