BMAL1 (ARNTL) (NM_001178) Human Tagged ORF Clone

CAT#: RC207870

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ARNTL (Myc-DDK-tagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 1



  "NM_001178" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ARNTL
Synonyms bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGACCAGAGAATGGACATTTCTTCAACCATCAGTGATTTCATGTCCCCGGGCCCCACCGACCTGC
TTTCCAGCTCTCTTGGTACCAGTGGTGTGGATTGCAACCGCAAACGGAAAGGCAGCTCCACTGACTACCA
AGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACACCAAGGAAGGATA
AAAAATGCAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATAAAATGAACAGTTTTATAGATG
AATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCAGGAAATTAGATAAACTTACTGTGCTAAGGAT
GGCTGTTCAGCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCAAACTACAAACCAACT
TTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTTTTGTTTGTCGTAGGAT
GTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCTCAACTACAGCCAGAATGATCT
GATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGATATTGCCAAAGTCAAGGAGCAGCTCTCCTCC
TCTGACACCGCACCCCGGGAGCGGCTCATAGATGCAAAAACTGGACTTCCAGTTAAAACAGATATAACCC
CTGGGCCATCTCGATTATGTTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGTGTAACAGGCCTTC
AGTAAAGGTTGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAAGATCGAAAAAGCTTCTGCACA
ATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGATGAAGACAACGAACCAG
ACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGACTGCATTCTCATGTAGTTCCACAACC
AGTGAACGGGGAAATCAGGGTGAAATCTATGGAATATGTTTCTCGGCACGCGATAGATGGAAAGTTTGTT
TTTGTAGACCAGAGGGCAACAGCTATTTTGGCATATTTACCACAAGAACTTCTAGGCACATCGTGTTATG
AATATTTTCACCAAGATGACATAGGACATCTTGCAGAATGTCATAGGCAAGTTTTACAGACGAGAGAAAA
AATTACAACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTATCACACTACGGAGTCGATGGTTC
AGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATTGTCTCAACTAACACTGTTGTTTTAGCCAACG
TCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCCCCACAGCATGGACAGCATGCTGCC
CTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCACTGTTCCAGGGATTCCAGGGGGAACCCGGGCTGGG
GCAGGAAAAATAGGCCGAATGATTGCTGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGCCTT
CTAGCTGTGGCTCCAGCCCATTGAACATCACGAGTACGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAA
GAAGATTTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGCCTACTATCAGGCCAGGCTCAGGAGAAC
CCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGGTATAGACATGATTG
ACAACGACCAAGGATCAAGTAGTCCCAGTAATGATGAGGCAGCAATGGCTGTCATCATGAGCCTCTTGGA
AGCAGATGCTGGACTGGGTGGCCCTGTTGACTTTAGTGACTTGCCATGGCCGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207870 protein sequence
Red=Cloning site Green=Tags(s)

MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRI
KNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPT
FLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSS
SDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCT
IHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSHVVPQPVNGEIRVKSMEYVSRHAIDGKFV
FVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFITLRSRWF
SFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTASPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRAG
AGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSSGLLSGQAQEN
PGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001178
ORF Size 1875 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001178.5
RefSeq Size 2863 bp
RefSeq ORF 1878 bp
Locus ID 406
UniProt ID O00327
Cytogenetics 11p15.3
Domains PAS, HLH, PAC
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Circadian rhythm - mammal
MW 68.7 kDa
Gene Summary The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

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