BMAL1 (ARNTL) (NM_001178) Human Untagged Clone

SKU SC119378

ARNTL (untagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BMAL1
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001178 unedited; NGGTGCAAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGAGCC; ACGGTGGTGCTGGCTAGAGTGTATACGTTTGGACCCAAGCTTAACTTTTCCAATGTGGAA; TCCTGGGCCTTCATTGGTTTTTGGAAGAAGCTGACCGCCTGAAAAGAAATTATAAAACAT; GAAAATCGCTTTGAGGTGACCAAGTCCAGAGGCCCCTAACTCCTCCCAAGCTGGATCTGG; GGTGTAAGAACTGTGACTTCAGATCATCCAATGGCAGACCAGAGAATGGACATTTCTTCA; ACCATCAGTGATTTCATGTCCCCGGGCCCCACCGACCTGCTTTCCAGCTCTCTTGGTACC; AGTGGTGTGGATTGCAACCGCAAACGGAAAGGCAGCTCCACTGACTACCATTCACAGGTC; GAATTTGGGGAGCACAATGGCTGGAGGTCAGATGCCCACTAGGAGATGCTATGATTAATA; TAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACACC; AAGGAAGGATAAAAAATGCAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATA; AAATGAACAGTTTTATAGATGAATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCA; GGAAATTAGATAAACTTACTGTGCTAAGGATGGCTGTTCAGCACATGAAAACATTAAGAG; GTGCCACCAATCCATACACAGAAGCAAACTACAAACCAACTTTNTTATCAGACGATGAAT; TGAAACACCTCATTCTCAGGNCAGCAGATGGATTTTTGTTTGTCGTAGGATGTGACCGAG; GGAAGATACTCTNTGTCTCAGAGTCTGTCTTCAGATCCTCACTACAGCAGAATGATCTGA; NTGGTCANAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001178 unedited; CGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTATAGAACAAGGGAAACATTTATTAA; AAATATTTAACTGTCTAGTACATTATACTTTAACCCATATTTATAGTAAAGGATTCTTAC; AAGGAAGAATAAACGGCTTTATGATACAATTAGTATAAGAAATATCCACATGGGGGACTT; CTTTGTAGTGTAAAAACACCATACATCTGAAATGACCATTTGCATATAAAAGCTACCAAT; GATGCTTCTGTGCACAATGATTAAAAAATTCAACACAAACAGAATCCAAAATATACAATT; AATGCAAGCTATCCACAGCTAGCCCAATAATCAATGATGAGGAAACACTGGAGCAGGCTT; AGTTCCACTTTGTCTGAAGTTACATATAGTAAGATTGACTTGTATCAATGGCTCTGAGAT; GGCTTTTATGAAGTCAGTACATAAAAGCTATTCTCTCCTTATCCAGTAAGCTTCACAGAC; TGTAAAACTCCACCAGTAATGCACTTTGATACTATAGCTGTTGCCAAAGCAACATGTAGT; GTTTACAGCGGCCATGGCAAGTCACTAAAGTCAACAGGGCCACCCAGTCCAGCATCTGCT; TCCAGAGGCTCATGATGACAGCCATTGCTGCCTCATCATTACTGGGACNTACTGATCCTT; GGTCGTTGNCAATCATGTCTATACCTATGTGGAGTTTCTCACCAGAATAGAAGAACTATC; AGATTATGGATAACCTGGGTTCTCCTGAGCCTGGCCTGATAGTAAGCACTGGGAGGAATG; TCTGGAGTCCCTCCATTTAAATCTTCTTGCTCCTGGAGAGAAGCATTAGGGGGAAGCGGA; CTCGTGATGTTCAATGGGCTGGAGCCCAGCTAGATGCAATGACCNTCTATCCTGGGATTT; CCATGATTCCTCAAATTATTCGGCTATTTCNCTGCCCACCCGGGTCCCCTGGATCCTGAA; CAAGGGGAGGTTCTCTTGGACACCTTTCAAAGGCAAATGCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001178
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001178.4, NP_001169.3
• RefSeq Size: 2812 bp
• RefSeq ORF: 1878 bp
• Locus ID: 406
• UniProt ID: O00327
• Cytogenetics: 11p15.3
• Domains: HLH, PAC, PAS
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]; Transcript Variant: This variant (1) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 4. The resulting isoform (a) is 1 aa shorter compared to isoform c. Variants 1, 2, 10, and 11 all encode isoform a.