Cytochrome p450 2J2 (CYP2J2) (NM_000775) Human Tagged ORF Clone

CAT#: RC207417

CYP2J2 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2J2 (Cat# RC207417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2(Cat# TA503636). Positive lysates LY424525 (100ug) and LC424525 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207417 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CYP2J2 protein (Cat# TP307417). The protein was produced from HEK293T cells transfected with CYP2J2 cDNA clone (Cat# RC207417) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP2J2
• Synonyms: CPJ2; CYPIIJ2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207417 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCGGCGATGGGCTCTCTGGCGGCTGCCCTCTGGGCAGTGGTCCATCCTCGGACTCTCCTACTGG
  GCACTGTCGCCTTTCTGCTCGCTGCTGACTTTCTCAAAAGACGGCGCCCAAAGAACTACCCGCCGGGGCC
  CTGGCGCCTGCCCTTCCTTGGCAACTTCTTCCTTGTGGACTTCGAGCAGTCGCACCTGGAGGTTCAGCTG
  TTTGTGAAGAAATATGGGAACCTTTTTAGCTTGGAGCTTGGTGACATATCTGCAGTTCTTATTACTGGCT
  TGCCCTTAATCAAAGAAGCCCTTATCCACATGGACCAAAACTTTGGGAACCGCCCCGTGACCCCTATGCG
  AGAACATATCTTTAAGAAAAATGGATTGATTATGTCAAGTGGCCAGGCATGGAAGGAGCAAAGAAGGTTC
  ACTCTGACAGCACTAAGGAACTTTGGTTTAGGAAAGAAGAGCTTAGAGGAACGCATTCAGGAGGAGGCCC
  AACACCTCACTGAAGCAATAAAAGAGGAGAACGGACAGCCTTTTGACCCTCATTTCAAGATCAACAATGC
  AGTTTCCAATATCATTTGCTCCATCACCTTCGGAGAACGCTTTGAGTACCAGGATAGTTGGTTTCAGCAG
  CTGCTGAAGTTACTAGATGAAGTCACATACTTGGAGGCTTCAAAGACATGCCAGCTCTACAATGTCTTTC
  CATGGATAATGAAATTCCTGCCTGGACCCCACCAAACTCTCTTCAGCAACTGGAAAAAACTGAAATTGTT
  TGTTTCTCATATGATTGACAAACACAGAAAGGATTGGAATCCTGCAGAAACAAGAGACTTTATTGATGCT
  TACCTTAAAGAAATGTCAAAGCACACAGGCAATCCTACTTCAAGTTTCCATGAAGAAAACCTCATCTGCA
  GCACCCTGGACCTCTTCTTTGCCGGAACCGAGACAACTTCCACAACTCTGCGATGGGCTCTGCTTTATAT
  GGCCCTCTACCCAGAAATCCAAGAAAAAGTACAAGCTGAGATTGACAGAGTGATTGGCCAGGGGCAGCAG
  CCGAGCACAGCCGCCCGGGAGTCCATGCCCTACACCAATGCTGTCATCCATGAGGTGCAGAGAATGGGCA
  ACATCATCCCCCTGAACGTTCCCAGGGAAGTGACAGTTGATACCACTTTGGCTGGGTACCACCTGCCCAA
  GGGTACCATGATCCTGACCAATTTGACGGCGCTGCACAGGGACCCCACAGAGTGGGCCACCCCTGACACA
  TTCAATCCGGACCATTTTCTGGAGAATGGACAGTTTAAGAAAAGGGAAGCCTTTATGCCTTTCTCAATAG
  GAAAGCGGGCATGCCTCGGAGAACAGTTGGCCAGGACTGAGCTGTTTATTTTCTTCACTTCCCTTATGCA
  AAAATTTACCTTCAGGCCCCCAAACAATGAGAAGCTGAGCCTGAAGTTTAGAATGGGTATCACCATTTCC
  CCAGTCAGTCACCGCCTCTGCGCTGTTCCTCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207417 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLAAMGSLAAALWAVVHPRTLLLGTVAFLLAADFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQL
  FVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRF
  TLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQ
  LLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDA
  YLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQ
  PSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDT
  FNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITIS
  PVSHRLCAVPQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000775
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000775.4
• RefSeq Size: 1876 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1573
• UniProt ID: P51589
• Cytogenetics: 1p32.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways
• MW: 57.6 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]