Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone

CAT#: RC207051

KCNJ12 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)

Product Images

Circular map for RC207051

• 

  Western blot validation of overexpression lysate (Cat# LY412138) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207051 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kir2.2
• Synonyms: hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207051 representing NM_021012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTGCACCTGGTCA
  CCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGT
  CAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGAC
  ATGTTCACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCT
  CCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGG
  CCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG
  CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGG
  CCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCC
  CAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCCGTGGTGGCCCTGCGTGACGGCAAGCTCTGC
  CTCATGTGGCGTGTGGGTAACCTGCGCAAGAGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCA
  AGCCGCGGGTCACCGAGGAGGGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAA
  GGGCCTGGACCGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC
  TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAAGGCATGGTGG
  AGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTT
  TGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATTGACTACTCGCACTTCCACAAGACCTATGAG
  GTGCCCTCTACGCCCCGCTGCAGTGCGAAGGATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACT
  CCTTCTGCTACGAGAACGAGCTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGA
  CGGCCGAAGCCGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG
  GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207051 representing NM_021012
  Red=Cloning site Green=Tags(s)
  
  MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLAD
  MFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVMQVHGFMAAFLFSIET
  QTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLC
  LMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPL
  FGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYE
  VPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGG
  VLEQRPYRRESEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021012
• ORF Size: 1299 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021012.5
• RefSeq Size: 5230 bp
• RefSeq ORF: 1302 bp
• Locus ID: 3768
• UniProt ID: Q14500
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 48.8 kDa
• Gene Summary: This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]