NXF1 (NM_006362) Human Tagged ORF Clone

CAT#: RC207039

NXF1 (Myc-DDK-tagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1

Product Images

Circular map for RC207039

• 

  Western blot validation of overexpression lysate (Cat# LY401913) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207039 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NXF1
• Synonyms: MEX67; TAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207039 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCCCTCAAAGAAAGAAGA
  AAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTAGGTCTGGAAGAGGCGGTTCTGGTAT
  TCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATGTGGCAATGAGTGATGCCCAGGATGGTCCCCGAGTA
  CGATACAACCCCTATACCACCCGACCTAACCGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATG
  TTACTGTGCGGAGAGACAGAGCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAA
  GAACTGGTTCAAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGATTCAG
  AGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGGCCCAGTTCTTCGTTG
  AAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGATTTTGGATCGGGAGAACCGAAGGAT
  ATCTATCATCATCAACTCTTCTGCTCCACCCCACACTATACTGAATGAACTGAAGCCAGAACAAGTAGAA
  CAGCTAAAGCTGATCATGAGCAAACGATACGATGGCTCCCAACAAGCCCTTGACCTCAAAGGCCTCCGTT
  CAGACCCAGATTTGGTGGCCCAGAACATTGACGTTGTCCTGAATCGCAGAAGCTGTATGGCAGCTACCCT
  GAGGATCATTGAAGAGAACATCCCTGAGCTATTGTCCTTGAACTTGAGCAACAACAGGCTGTACAGGCTG
  GATGACATGTCTAGCATTGTTCAGAAGGCACCCAACCTGAAGATCCTAAACCTTTCTGGAAATGAATTGA
  AGTCTGAGCGGGAATTGGACAAGATAAAGGGGCTGAAGCTAGAAGAGCTCTGGCTCGATGGAAACTCCCT
  GTGTGACACCTTCCGAGACCAGTCCACCTACATCAGCGCCATTCGCGAACGATTTCCCAAGTTACTACGC
  CTGGATGGCCATGAGCTACCCCCACCAATTGCCTTTGATGTTGAAGCCCCCACGACGTTACCGCCCTGCA
  AGGGAAGCTATTTTGGAACAGAAAACTTGAAGAGTCTGGTCTTGCACTTCCTGCAACAGTACTATGCAAT
  TTACGACTCTGGAGACCGACAAGGGCTCCTGGATGCCTACCATGATGGGGCCTGCTGTTCCCTGAGCATT
  CCTTTCATTCCTCAGAACCCTGCCCGAAGCAGCTTAGCCGAGTATTTCAAGGATAGCAGAAATGTGAAGA
  AGCTTAAAGACCCTACCTTGCGGTTCCGGCTGCTGAAGCACACGCGTCTCAACGTTGTTGCCTTCCTCAA
  TGAGTTGCCCAAAACCCAGCACGACGTCAATTCCTTCGTGGTAGACATAAGCGCCCAGACAAGCACATTG
  CTGTGTTTTTCTGTCAATGGAGTCTTCAAGGAAGTGGACGGAAAGTCCCGGGATTCTTTGCGAGCCTTCA
  CCCGGACATTCATTGCTGTTCCTGCTAGCAATTCAGGGCTATGTATTGTAAATGATGAGCTATTTGTGCG
  GAATGCCAGTTCTGAAGAGATCCAAAGAGCCTTCGCTATGCCTGCACCCACGCCTTCCTCCAGCCCGGTG
  CCCACCCTCTCTCCAGAGCAGCAGGAAATGTTGCAAGCATTCTCTACCCAGTCTGGCATGAACCTCGAGT
  GGTCCCAGAAGTGCCTTCAGGACAACAACTGGGACTACACCAGATCTGCCCAGGCCTTCACTCATCTCAA
  GGCCAAGGGCGAGATCCCAGAAGTGGCATTCATGAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207039 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRV
  RYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQ
  SKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVE
  QLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRL
  DDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLR
  LDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSI
  PFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTL
  LCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPV
  PTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006362
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006362.5
• RefSeq Size: 2346 bp
• RefSeq ORF: 1860 bp
• Locus ID: 10482
• UniProt ID: Q9UBU9
• Cytogenetics: 11q12.3
• Domains: LRR, NTF2, TAP_C
• MW: 70.2 kDa
• Gene Summary: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]