IQCF1 (NM_152397) Human Tagged ORF Clone

CAT#: RC206941

IQCF1 (Myc-DDK-tagged)-Human IQ motif containing F1 (IQCF1)

Product Images

Circular map for RC206941

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  Western blot validation of overexpression lysate (Cat# LY407565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206941 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IQCF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206941 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGAAGCAGCCCCAAAAGACGAAGGAACCCTCAAAAGAAGATGAGCCTCAGCAGAAGGAGATGC
  CAACCCATTTGTCCTTAGGAGCAGAGTCAAAGGCAGAGGCTAAAACTCCAGTTCTGGTTGAGACACAGAC
  AGTGGACAATGCCAATGAGAAATCAGAAAAACCCCCAGAAAACCAAAAGAAGCTCTCTGACAAAGATACG
  GTAGCCACCAAGATCAAGGCCTGGTGGCGGGGCACCCTGGTGCGTCGGGCACTGTTGCACGCAGCCCTCA
  GTGCCTGCATCATTCAGTGCTGGTGGCGGCTGATACTGTCCAAGATTCTGAAGAAGAGGCGGCAGGCAGC
  GCTAGAGGCCTTCTCCCGGAAGGAGTGGGCAGCAGTCACACTGCAGTCCCAGGCCCGCATGTGGCGCATC
  CGCAGACGCTATTGCCAGGTGCTCAATGCTGTTCGCATCATCCAGGCTTACTGGAGGTGCCGCTCCTGTG
  CTTCCCGGGGGTTCATCAAGGGCCAGTACAGAGTCACAGCCAACCAGCTGCATCTCCAGCTGGAGATCTT
  GCTGGACTCAGGGCCTTGCATTGTGACAGAGTGTATTCCCTTCTCAATAAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206941 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEKQPQKTKEPSKEDEPQQKEMPTHLSLGAESKAEAKTPVLVETQTVDNANEKSEKPPENQKKLSDKDT
  VATKIKAWWRGTLVRRALLHAALSACIIQCWWRLILSKILKKRRQAALEAFSRKEWAAVTLQSQARMWRI
  RRRYCQVLNAVRIIQAYWRCRSCASRGFIKGQYRVTANQLHLQLEILLDSGPCIVTECIPFSIKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152397
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152397.1, NP_689610.1
• RefSeq Size: 745 bp
• RefSeq ORF: 618 bp
• Locus ID: 132141
• UniProt ID: Q8N6M8
• Cytogenetics: 3p21.2
• MW: 23.7 kDa
• Gene Summary: Involved in sperm capacitation and acrosome reaction.[UniProtKB/Swiss-Prot Function]