ZCCHC12 (NM_173798) Human Tagged ORF Clone

CAT#: RC206924

ZCCHC12 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 12 (ZCCHC12)

Product Images

Circular map for RC206924

• 

  Western blot validation of overexpression lysate (Cat# LY406574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206924 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZCCHC12
• Synonyms: PNMA7A; SIZN; SIZN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206924 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGCATCATTGCACGTGTCGGTAACAGCCGGCGGCTGAATGCACCCTTGCCGCCTTGGGCCCATT
  CCATGCTGAGGTCCCTGGGGAGAAGTCTCGGTCCTATAATGGCCAGCATGGCAGACAGAAACATGAAGTT
  GTTCTCGGGGAGGGTGGTGCCAGCCCAAGGGGAAGAAACCTTTGAAAACTGGCTGACCCAAGTCAATGGC
  GTCCTGCCAGATTGGAATATGTCTGAGGAGGAAAAGCTCAAGCGCTTGATGAAAACCCTTAGGGGCCCTG
  CCCGCGAGGTCATGCGTGTGCTTCAGGCGACCAACCCTAACCTAAGTGTGGCAGATTTCTTGCGAGCCAT
  GAAATTGGTGTTTGGGGAGTCTGAAAGCAGTGTGACTGCCCATGGTAAATTTTTTAACACCCTACAAGCT
  CAAGGGGAGAAAGCCTCCCTTTATGTGATCCGTTTAGAGGTGCAGCTCCAGAACGCTATTCAGGCAGGCA
  TTATAGCTGAGAAAGATGCAAACCGGACTCGCTTGCAGCAGCTCCTTTTAGGCGGTGAGCTGAGTAGGGA
  CCTCCGACTCAGACTTAAGGATTTTCTCAGGATGTATGCAAATGAGCAGGAGCGGCTTCCCAACTTTCTG
  GAGTTAATCAGAATGGTAAGGGAGGAAGAGGATTGGGATGATGCTTTTATTAAACGGAAGCGTCCAAAAA
  GGTCTGAGTCAATGGTGGAGAGGGCAGTCAGCCCTGTGGCATTTCAGGGCTCCCCACCGATAGTGATCGG
  CAGTGCTGACTGCAATGTGATAGAGATAGATGATACCCTCGACGACTCCGATGAGGATGTGATCCTGGTG
  GAGTCTCAGGACCCTCCACTTCCATCCTGGGGTGCCCCTCCCCTCAGAGACAGGGCCAGACCTCAGGATG
  AAGTGCTGGTCATTGATTCCCCCCACAATTCCAGGGCTCAGTTTCCTTCCACCAGTGGTGGTTCTGGCTA
  TAAGAATAACGGTCCTGGGGAGATGCGTAGAGCCAGGAAGCGAAAACACACAATCCGCTGTTCGTATTGT
  GGTGAGGAAGGCCACTCAAAAGAAACCTGTGACAACGAGAGTGACAAGGCCCAGGTTTTTGAGAATTTGA
  TCATCACTCTCCAGGAGCTGACCCATACTGAGATGGAGAGGTCAAGAGTGGCCCCTGGCGAATACAATGA
  CTTCTCTGAGCCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206924 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASIIARVGNSRRLNAPLPPWAHSMLRSLGRSLGPIMASMADRNMKLFSGRVVPAQGEETFENWLTQVNG
  VLPDWNMSEEEKLKRLMKTLRGPAREVMRVLQATNPNLSVADFLRAMKLVFGESESSVTAHGKFFNTLQA
  QGEKASLYVIRLEVQLQNAIQAGIIAEKDANRTRLQQLLLGGELSRDLRLRLKDFLRMYANEQERLPNFL
  ELIRMVREEEDWDDAFIKRKRPKRSESMVERAVSPVAFQGSPPIVIGSADCNVIEIDDTLDDSDEDVILV
  ESQDPPLPSWGAPPLRDRARPQDEVLVIDSPHNSRAQFPSTSGGSGYKNNGPGEMRRARKRKHTIRCSYC
  GEEGHSKETCDNESDKAQVFENLIITLQELTHTEMERSRVAPGEYNDFSEPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173798
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173798.4
• RefSeq Size: 2212 bp
• RefSeq ORF: 1209 bp
• Locus ID: 170261
• UniProt ID: Q6PEW1
• Cytogenetics: Xq24
• MW: 45.4 kDa
• Gene Summary: This gene encodes a downstream effector of bone morphogenetic protein (BMP) signalling. This protein contains a zinc finger domain and functions as a transcriptional coactivator. Variation in this gene may be associated with X-linked cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]