CDS2 (NM_003818) Human Tagged ORF Clone

CAT#: RC206807

CDS2 (Myc-DDK-tagged)-Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 (CDS2)

Product Images

Circular map for RC206807

• 

  Western blot validation of overexpression lysate (Cat# LY418419) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206807 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206807 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAGCTGAGGCAGAGGGTGGCCCATGAGCCGGTTGCGCCACCCGAGGACAAGGAGTCAGAGTCAG
  AAGCAAAGGTAGATGGAGAGACTGCATCGGACAGTGAGAGCCGGGCAGAATCCGCACCCCTGCCAGTCTC
  TGCAGATGATACCCCGGAGGTCCTCAATAGGGCCCTTTCCAACTTGTCTTCAAGATGGAAGAACTGGTGG
  GTGAGAGGCATCCTGACTTTGGCCATGATTGCATTTTTCTTCATCATCATTTACCTGGGACCAATGGTTT
  TGATGATAATCGTGATGTGCGTTCAGATTAAGTGTTTCCATGAGATAATCACTATTGGCTACAACGTCTA
  CCACTCATATGATCTGCCCTGGTTCAGGACGCTCAGCTGGTACTTTCTCCTGTGTGTAAACTATTTCTTC
  TATGGTGAGACAGTGACGGATTACTTCTTCACCCTGGTCCAGAGAGAAGAGCCTTTGCGGATTCTCAGTA
  AATACCACCGGTTCATTTCCTTTACTCTCTATCTAATAGGATTCTGCATGTTTGTACTGAGTCTGGTCAA
  GAAGCATTATCGACTGCAGTTCTACATGTTTGGCTGGACCCATGTGACATTGCTGATTGTTGTAACACAG
  TCACATCTTGTTATCCACAACCTATTTGAAGGAATGATCTGGTTCATTGTCCCCATATCTTGTGTGATCT
  GTAATGACATCATGGCCTATATGTTTGGCTTTTTCTTTGGTCGGACCCCACTCATCAAGCTGTCCCCGAA
  GAAGACCTGGGAAGGCTTCATTGGGGGCTTCTTTGCTACTGTGGTGTTTGGCCTTCTGCTGTCCTATGTG
  ATGTCCGGGTACAGATGCTTTGTCTGCCCTGTGGAGTACAACAATGACACCAACAGCTTCACTGTGGACT
  GTGAGCCCTCGGACCTGTTTCGCCTGCAGGAGTACAACATTCCTGGGGTGATCCAGTCAGTCATTGGCTG
  GAAAACGGTCCGGATGTACCCCTTCCAGATTCACAGCATCGCTCTCTCCACCTTTGCCTCGCTCATTGGC
  CCCTTTGGAGGATTCTTCGCAAGTGGATTCAAACGAGCCTTTAAAATCAAAGACTTTGCCAATACCATTC
  CTGGCCATGGAGGCATCATGGATCGCTTTGACTGCCAGTATCTGATGGCCACCTTTGTCAATGTATACAT
  CGCCAGTTTTATCAGAGGCCCTAACCCAAGCAAACTGATTCAGCAGTTCCTGACTTTACGGCCAGATCAG
  CAGCTCCACATCTTCAACACGCTGCGGTCTCATCTGATCGACAAAGGGATGCTGACATCCACCACAGAGG
  ACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206807 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTELRQRVAHEPVAPPEDKESESEAKVDGETASDSESRAESAPLPVSADDTPEVLNRALSNLSSRWKNWW
  VRGILTLAMIAFFFIIIYLGPMVLMIIVMCVQIKCFHEIITIGYNVYHSYDLPWFRTLSWYFLLCVNYFF
  YGETVTDYFFTLVQREEPLRILSKYHRFISFTLYLIGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQ
  SHLVIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATVVFGLLLSYV
  MSGYRCFVCPVEYNNDTNSFTVDCEPSDLFRLQEYNIPGVIQSVIGWKTVRMYPFQIHSIALSTFASLIG
  PFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPSKLIQQFLTLRPDQ
  QLHIFNTLRSHLIDKGMLTSTTEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003818
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003818.4
• RefSeq Size: 9323 bp
• RefSeq ORF: 1338 bp
• Locus ID: 8760
• UniProt ID: O95674
• Cytogenetics: 20p12.3
• Domains: CTP_transf_1
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 51.4 kDa
• Gene Summary: Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. [provided by RefSeq, Jul 2008]