CDS2 (NM_003818) Human Untagged Clone

CAT#: SC120671

CDS2 (untagged)-Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 (CDS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120671 sequence for NM_003818 edited (data generated by NextGen Sequencing)
  ATGACAGAGCTGAGGCAGAGGGTGGCCCATGAGCCGGTTGCGCCACCCGAGGACAAGGAG
  TCAGAGTCAGAAGCAAAGGTAGATGGAGAGACTGCATCGGACAGTGAGAGCCGGGCAGAA
  TCCGCACCCCTGCCAGTCTCTGCAGATGATACCCCGGAGGTCCTCAATAGGGCCCTTTCC
  AACTTGTCTTCAAGATGGAAGAACTGGTGGGTGAGAGGCATCCTGACTTTGGCCATGATT
  GCATTTTTCTTCATCATCATTTACCTGGGACCAATGGTTTTGATGATAATCGTGATGTGC
  GTTCAGATTAAGTGTTTCCATGAGATAATCACTATTGGCTACAACGTCTACCACTCATAT
  GATCTGCCCTGGTTCAGGACGCTCAGCTGGTACTTTCTCCTGTGTGTAAACTATTTCTTC
  TATGGTGAGACAGTGACGGATTACTTCTTCACCCTGGTCCAGAGAGAAGAGCCTTTGCGG
  ATTCTCAGTAAATACCACCGGTTCATTTCCTTTACTCTCTATCTAATAGGATTCTGCATG
  TTTGTACTGAGTCTGGTCAAGAAGCATTATCGACTGCAGTTCTACATGTTTGGCTGGACC
  CATGTGACATTGCTGATTGTTGTAACACAGTCACATCTTGTTATCCACAACCTATTTGAA
  GGAATGATCTGGTTCATTGTCCCCATATCTTGTGTGATCTGTAATGACATCATGGCCTAT
  ATGTTTGGCTTTTTCTTTGGTCGGACCCCACTCATCAAGCTGTCCCCGAAGAAGACCTGG
  GAAGGCTTCATTGGGGGCTTCTTTGCTACTGTGGTGTTTGGCCTTCTGCTGTCCTATGTG
  ATGTCCGGGTACAGATGCTTTGTCTGCCCTGTGGAGTACAACAATGACACCAACAGCTTC
  ACTGTGGACTGTGAGCCCTCGGACCTGTTTCGCCTGCAGGAGTACAACATTCCTGGGGTG
  ATCCAGTCAGTCATTGGCTGGAAAACGGTCCGGATGTACCCCTTCCAGATTCACAGCATC
  GCTCTCTCCACCTTTGCCTCGCTCATTGGCCCCTTTGGAGGATTCTTCGCAAGTGGATTC
  AAACGAGCCTTTAAAATCAAAGACTTTGCCAATACCATTCCTGGCCATGGAGGCATCATG
  GATCGCTTTGACTGCCAGTATCTGATGGCCACCTTTGTCAATGTATACATCGCCAGTTTT
  ATCAGAGGCCCTAACCCAAGCAAACTGATTCAGCAGTTCCTGACTTTACGGCCAGATCAG
  CAGCTCCACATCTTCAACACGCTGCGGTCTCATCTGATCGACAAAGGGATGCTGACATCC
  ACCACAGAGGACGAGTAG
  
  Clone variation with respect to NM_003818.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003818 unedited
  NGGTCACTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGGGCTGC
  TAAGGGAACTGTGAGCCGCTCAGAGCCGCGCGCCTCCCGGGCGGGGCGGGGCCGGCCGTG
  GGAGTCCGCGCGTGCCCGCGCCGAGCTGCCTGCTCCGGCGGCTTCGCTGCTAGCTCGCGG
  CGACGTCGGGCCGATTTTCCCAGGATGACAGAGCTGAGGCAGAGGGTGGCCCATGAGCCG
  GTTGCGCCACCCGAGGACAAGGAGTCAGAGTCAGAAGCAAAGGTAGATGGAGAGACTGCA
  TCGGACAGTGAGAGCCGGGCAGAATCCGCACCCCTGCCAGTCTCTGCAGATGATACCCCG
  GAGGTCCTCAATAGGGCCCTTTCCAACTTGTCTTCAAGATGGAAGAACTGGTGGGTGAGA
  GGCATCCTGACTTTGGCCATGATTGCATTTTTCTTCATCATCATTTACCTGGGACCAATG
  GTTTTGATGATAATCGTGATGTGCGTTCAGATTAAGTGTTTCCATGAGATAATCACTATT
  GGCTACAACGTCTACCACTCATATGATCTGCCCTGGTTCAGGACGCTCAGCTGGTACTTT
  CTCCTGTGTGTAAACTATTTCTTCTATGGTGAGACAGTGACGGATTACTTCTTCACCCTG
  GTCCAGAGAGAAGAGCCTTTGCGGATTCTCAGTAAATACCACCGGTTCATTTCCTTTACT
  CTCTATCTAATAGGATTCTGCATGTTTGTACTGAGTCTGGTCAAGAAGCATTATCGACTG
  CAGTTCTACATGTTTGGGCTGGACCCATGTGACATTGCTGATTGGTGTAACACAGTCACA
  TCTTGTTATCCACAACCTATTTGAAGGAATGATCTGGNTCATTGTCCCATATCTTGTGTG
  ATCTGTAATGACATCATGCNCCTATATGTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003818 unedited
  NNTTTTCGCTCTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAC
  ACATTATATAAAAGTGTGCATTTAATCTTCAAAATAGTCAAGGCTCTAATCAGGTTAGGT
  TTTCCATAGTTTTAAGCAGGACTTTGTGGTTTTAGTGAAGAAGTCATGGTGCAATTGAAA
  TCACTGTAAGAAATAAGTGACTTTTAAAACAAACACAGACACACACACTCCTCTTAAGAG
  TAATATATACACAACACAGCAGCTACATGGGTGTTCAGGCAAAGGGTGCATGAACGAGAA
  GCCCTCTGCTCCCTGCCCGATGAGAAAGTCCCCAGAAAGGATTCAGCAGCAGCAAGTCTA
  CAGCACAAACATGGATGGCATTGTCCCTGAAAACACACAGTTAGGTGGACCTACAGGAGA
  CATTGGAGCCTAGACATGTGGGAAAGGGCTCAGTTACAGTACATTCTACTGCATACACTT
  GAAATATTACAGTGTGTTTTTTCTCCAGACTATTATAAATAATTTTTCGTGCTTTCTGAA
  AAAAATAAAAACTGAAACTTTCAGTCTGCGATGAAGGTGAACCCATCTTATAAAGCAGAG
  CTTACTTACATTCTGCAGGATTTTGGTGTGGATGCATAGAAGGCTTACCTGGTTAGTAAG
  CCTCCATTCCTCCGACCTACAGAAGGCAACCCTTCTGCAGCTCCAAGCAGGGATTTCTAG
  AAAAGACCAGGTGTACAAAACATCTAAGCTGTGCCCTCCACCCACAGTGGGGAAGTCCCC
  TGATTGGTCAGACCAGATGATGTCTGTGTCTGACTGACCCCAAAACAGTTCATTTGTGGG
  GGCTGGAACCACCAAAAAACCTTCTGACCTGGGCAACATTGCTTCCAAATATTATACTTT
  TGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003818
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003818.2, NP_003809.1
• RefSeq Size: 2711 bp
• RefSeq ORF: 1338 bp
• Locus ID: 8760
• UniProt ID: O95674
• Cytogenetics: 20p12.3
• Domains: CTP_transf_1
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. [provided by RefSeq, Jul 2008]