RDHE2 (SDR16C5) (NM_138969) Human Tagged ORF Clone

CAT#: RC206759

SDR16C5 (Myc-DDK-tagged)-Human short chain dehydrogenase/reductase family 16C, member 5 (SDR16C5)

Product Images

Circular map for RC206759

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  Western blot validation of overexpression lysate (Cat# LY408444) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206759 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SDR16C5 protein (Cat# TP306759). The protein was produced from HEK293T cells transfected with SDR16C5 cDNA clone (Cat# RC206759) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RDHE2
• Synonyms: EPHD-2; RDH#2; RDH-E2; RDHE2; retSDR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206759 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTCAACCTGCAATCATCAAAGAAACTGTTCATTTTCTTAGGAAAATCACTGTTTAGTCTTCTGG
  AGGCTATGATTTTTGCCTTACTCCCAAAGCCACGGAAGAACGTTGCTGGTGAAATAGTCCTCATCACAGG
  TGCTGGAAGTGGACTCGGAAGGCTCTTAGCCTTGCAGTTTGCCCGGCTGGGATCTGTTCTTGTTCTCTGG
  GATATCAATAAGGAGGGGAATGAGGAAACATGTAAGATGGCTCGGGAAGCTGGAGCCACAAGAGTGCACG
  CCTATACCTGCGATTGCAGCCAAAAGGAAGGAGTGTATAGAGTAGCCGACCAGGTTAAAAAAGAAGTCGG
  CGATGTTTCCATCCTAATCAACAATGCCGGAATCGTAACAGGCAAAAAGTTCCTTGACTGTCCAGATGAG
  CTTATGGAAAAGTCATTTGATGTGAATTTCAAAGCACATTTATGGACTTATAAAGCCTTTCTACCTGCTA
  TGATTGCTAATGACCATGGACATTTGGTTTGCATTTCAAGTTCAGCTGGATTAAGTGGAGTAAATGGGCT
  GGCAGATTACTGTGCAAGTAAATTTGCAGCCTTTGGGTTTGCTGAATCTGTATTTGTAGAAACATTTGTC
  CAAAAACAAAAGGGGATCAAAACCACGATTGTGTGCCCCTTTTTTATAAAAACTGGAATGTTTGAAGGTT
  GTACTACAGGCTGTCCTTCTCTGTTGCCAATTCTGGAACCAAAATATGCAGTTGAAAAAATAGTAGAAGC
  TATTCTACAAGAAAAAATGTACTTGTATATGCCAAAGTTGTTATACTTCATGATGTTTCTTAAAAGCTTT
  TTGCCCCTCAAGACAGGACTGCTTATAGCTGACTATTTGGGCATCCTTCATGCAATGGATGGCTTTGTTG
  ACCAAAAGAAGAAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206759 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFNLQSSKKLFIFLGKSLFSLLEAMIFALLPKPRKNVAGEIVLITGAGSGLGRLLALQFARLGSVLVLW
  DINKEGNEETCKMAREAGATRVHAYTCDCSQKEGVYRVADQVKKEVGDVSILINNAGIVTGKKFLDCPDE
  LMEKSFDVNFKAHLWTYKAFLPAMIANDHGHLVCISSSAGLSGVNGLADYCASKFAAFGFAESVFVETFV
  QKQKGIKTTIVCPFFIKTGMFEGCTTGCPSLLPILEPKYAVEKIVEAILQEKMYLYMPKLLYFMMFLKSF
  LPLKTGLLIADYLGILHAMDGFVDQKKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138969
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138969.3, NP_620419.2
• RefSeq Size: 3039 bp
• RefSeq ORF: 930 bp
• Locus ID: 195814
• UniProt ID: Q8N3Y7
• Cytogenetics: 8q12.1
• Protein Families: Druggable Genome
• MW: 34.1 kDa
• Gene Summary: This gene encodes a member of the short-chain alcohol dehydrogenase/reductase superfamily of proteins and is involved in the oxidation of retinol to retinaldehyde. The encoded protein is associated with the endoplasmic reticulum and is predicted to contain three transmembrane helices, suggesting that it is an integral membrane protein. It recognizes all-trans-retinol and all-trans-retinaldehyde as substrates and exhibits a strong preference for NAD(+)/NADH as cofactors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]