Parvin gamma (PARVG) (NM_022141) Human Tagged ORF Clone

CAT#: RC206755

PARVG (Myc-DDK-tagged)-Human parvin, gamma (PARVG), transcript variant 1

Product Images

Circular map for RC206755

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  Western blot validation of overexpression lysate (Cat# LY427945) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227051 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PARVG protein (Cat# TP306755). The protein was produced from HEK293T cells transfected with PARVG cDNA clone (Cat# RC206755) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parvin gamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206755 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGAGTTCTTGTACGACCTGCTGCAGCTCCCCAAGGGGGTGGAGCCCCCAGCGGAGGAGGAGC
  TCTCAAAAGGAGGAAAGAAGAAATACCTGCCACCCACTTCCCGGAAGGACCCCAAATTTGAAGAACTGCA
  GAAGGTGTTGATGGAGTGGATCAATGCCACTCTTCTCCCCGAGCACATTGTGGTCCGCAGCCTGGAGGAG
  GACATGTTCGACGGGCTCATCCTACACCACCTATTCCAGAGGCTGGCGGCGCTCAAGCTGGAAGCAGAGG
  ACATCGCCCTGACAGCCACAAGCCAGAAGCACAAGCTCACAGTGGTGCTGGAGGCCGTGAACCGGAGTCT
  GCAGCTGGAGGAGTGGCAGGCCAAGTGGAGCGTGGAGAGCATCTTCAACAAGGACCTGTTGTCTACCCTG
  CACCTCCTTGTGGCCCTGGCCAAGCGCTTCCAGCCCGACCTCTCCCTCCCAACCAACGTCCAGGTGGAGG
  TCATCACTATCGAGAGCACCAAAAGTGGTCTGAAGTCAGAGAAGTTGGTGGAACAGCTCACTGAATACAG
  CACAGACAAGGACGAGCCTCCAAAGGACGTCTTTGATGAATTATTTAAGCTGGCTCCGGAGAAAGTGAAC
  GCAGTGAAAGAGGCCATCGTGAACTTTGTCAACCAGAAGCTGGACCGCCTGGGCCTGTCTGTGCAGAATC
  TGGACACCCAGTTTGCAGATGGGGTCATCTTACTCTTGCTGATTGGACAACTTGAAGGCTTCTTCCTGCA
  CTTAAAGGAATTCTACCTCACTCCCAACTCTCCTGCAGAAATGCTGCACAACGTCACCCTGGCGCTGGAG
  CTGCTGAAGGACGAGGGCCTGCTCAGCTGCCCTGTCAGCCCTGAAGATATCGTGAACAAGGATGCCAAGA
  GCACACTGCGGGTGCTCTATGGTCTGTTCTGCAAGCACACGCAGAAGGCACACAGGGACAGGACGCCCCA
  TGGAGCCCCGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206755 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPEFLYDLLQLPKGVEPPAEEELSKGGKKKYLPPTSRKDPKFEELQKVLMEWINATLLPEHIVVRSLEE
  DMFDGLILHHLFQRLAALKLEAEDIALTATSQKHKLTVVLEAVNRSLQLEEWQAKWSVESIFNKDLLSTL
  HLLVALAKRFQPDLSLPTNVQVEVITIESTKSGLKSEKLVEQLTEYSTDKDEPPKDVFDELFKLAPEKVN
  AVKEAIVNFVNQKLDRLGLSVQNLDTQFADGVILLLLIGQLEGFFLHLKEFYLTPNSPAEMLHNVTLALE
  LLKDEGLLSCPVSPEDIVNKDAKSTLRVLYGLFCKHTQKAHRDRTPHGAPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022141
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022141.3
• RefSeq Size: 3535 bp
• RefSeq ORF: 996 bp
• Locus ID: 64098
• UniProt ID: Q9HBI0
• Cytogenetics: 22q13.31
• Domains: CH
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
• MW: 37.5 kDa
• Gene Summary: Members of the parvin family, including PARVG, are actin-binding proteins associated with focal contacts.[supplied by OMIM, Aug 2004]