MDC (CCL22) (NM_002990) Human Tagged ORF Clone
CCL22 (Myc-DDK-tagged)-Human chemokine (C-C motif) ligand 22 (CCL22)
|Type||Human Tagged ORF Clone|
|Synonyms||A-152E5.1; ABCD-1; DC/B-CK; MDC; SCYA22; STCP-1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC206578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206578 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||279 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2933 bp|
|RefSeq ORF||282 bp|
|Protein Families||Druggable Genome, Secreted Protein, Transmembrane|
|Protein Pathways||Chemokine signaling pathway, Cytokine-cytokine receptor interaction|
|Gene Summary||This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]|
|cDNA Clone Resources|
|RC206578L1||Lenti ORF clone of Human chemokine (C-C motif) ligand 22 (CCL22), Myc-DDK-tagged||
|RC206578L2||Lenti ORF clone of Human chemokine (C-C motif) ligand 22 (CCL22), mGFP tagged||
|RC206578L3||Lenti ORF clone of Human chemokine (C-C motif) ligand 22 (CCL22), Myc-DDK-tagged||
|RC206578L4||Lenti ORF clone of Human chemokine (C-C motif) ligand 22 (CCL22), mGFP tagged||
|RG206578||CCL22 (GFP-tagged) - Human chemokine (C-C motif) ligand 22 (CCL22)||
|SC321689||CCL22 (untagged)-Human chemokine (C-C motif) ligand 22 (CCL22)||