Apolipoprotein CIII (APOC3) (NM_000040) Human Tagged ORF Clone
APOC3 (Myc-DDK-tagged)-Human apolipoprotein C-III (APOC3)
|Product Name||Apolipoprotein CIII (APOC3) (NM_000040) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC206566 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206566 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||297 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||533 bp|
|RefSeq ORF||300 bp|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||PPAR signaling pathway|
|Gene Summary||This gene encodes a protein component of triglyceride (TG)-rich lipoproteins (TRLs) including very low density lipoproteins (VLDL), high density lipoproteins (HDL) and chylomicrons. The encoded protein plays a role in role in the metabolism of these TRLs through multiple modes. This protein has been shown to promote the secretion of VLDL1, inhibit lipoprotein lipase enzyme activity, and delay catabolism of TRL remnants. Mutations in this gene are associated with low plasma triglyceride levels and reduced risk of ischemic cardiovascular disease, and hyperalphalipoproteinemia, which is characterized by elevated levels of high density lipoprotein (HDL) and HDL cholesterol in human patients. This gene and other related genes comprise an apolipoprotein gene cluster on chromosome 11. [provided by RefSeq, Sep 2017]|
|The use of this cDNA Clones has been cited in the following citations:|
A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels
,Khetarpal, SA;Zeng, X;Millar, JS;Vitali, C;Somasundara, AVH;Zanoni, P;Landro, JA;Barucci, N;Zavadoski, WJ;Sun, Z;de Haard, H;Toth, IV;Peloso, GM;Natarajan, P;Cuchel, M;Lund-Katz, S;Phillips, MC;Tall, AR;Kathiresan, S;DaSilva-Jardine, P;Yates, NA;Rader, DJ;,
,PubMed ID 28825717
|cDNA Clone Resources|
|SC113775||APOC3 (untagged)-Human apolipoprotein C-III (APOC3)||
|RG206566||APOC3 (GFP-tagged) - Human apolipoprotein C-III (APOC3)||
|RC206566L1||Lenti ORF clone of Human apolipoprotein C-III (APOC3), Myc-DDK-tagged||
|RC206566L2||Lenti ORF clone of Human apolipoprotein C-III (APOC3), mGFP tagged||
|RC206566L3||Lenti ORF clone of Human apolipoprotein C-III (APOC3), Myc-DDK-tagged||
|RC206566L4||Lenti ORF clone of Human apolipoprotein C-III (APOC3), mGFP tagged||