BMP5 (NM_021073) Human Tagged ORF Clone

CAT#: RC206456

BMP5 (Myc-DDK-tagged)-Human bone morphogenetic protein 5 (BMP5)

Product Images

Circular map for RC206456

• 

  Western blot validation of overexpression lysate (Cat# LY402826) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206456 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206456 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTGACTGTATTTTTACTTAAGGGTATTGTGGGTTTCCTCTGGAGCTGCTGGGTTCTAGTGGGTT
  ATGCAAAAGGAGGTTTGGGAGACAATCATGTTCACTCCAGTTTTATTTATAGAAGACTACGGAACCACGA
  AAGACGGGAAATACAAAGGGAAATTCTCTCTATCTTGGGTTTGCCTCACAGACCCAGACCATTTTCACCT
  GGAAAACAAGCGTCCTCTGCACCTCTCTTTATGCTGGATCTCTACAATGCCATGACCAATGAAGAAAATC
  CTGAAGAGTCGGAGTACTCAGTAAGGGCATCCTTGGCAGAAGAGACCAGAGGGGCAAGAAAGGGATACCC
  AGCCTCTCCCAATGGGTATCCTCGTCGCATACAGTTATCTCGGACGACTCCTCTGACCACCCAGAGTCCT
  CCTCTAGCCAGCCTCCATGATACCAACTTTCTGAATGATGCTGACATGGTCATGAGCTTTGTCAACTTAG
  TTGAAAGAGACAAGGATTTTTCTCACCAGCGAAGGCATTACAAAGAATTTCGATTTGATCTTACCCAAAT
  TCCTCATGGAGAGGCAGTGACAGCAGCTGAATTCCGGATATACAAGGACCGGAGCAACAACCGATTTGAA
  AATGAAACAATTAAGATTAGCATATATCAAATCATCAAGGAATACACAAATAGGGATGCAGATCTGTTCT
  TGTTAGACACAAGAAAGGCCCAAGCTTTAGATGTGGGTTGGCTTGTCTTTGATATCACTGTGACCAGCAA
  TCATTGGGTGATTAATCCCCAGAATAATTTGGGCTTACAGCTCTGTGCAGAAACAGGGGATGGACGCAGT
  ATCAACGTAAAATCTGCTGGTCTTGTGGGAAGACAGGGACCTCAGTCAAAACAACCATTCATGGTGGCCT
  TCTTCAAGGCGAGTGAGGTACTTCTTCGATCCGTGAGAGCAGCCAACAAACGAAAAAATCAAAACCGCAA
  TAAATCCAGCTCTCATCAGGACTCCTCCAGAATGTCCAGTGTTGGAGATTATAACACAAGTGAGCAAAAA
  CAAGCCTGTAAGAAGCACGAACTCTATGTGAGCTTCCGGGATCTGGGATGGCAGGACTGGATTATAGCAC
  CAGAAGGATACGCTGCATTTTATTGTGATGGAGAATGTTCTTTTCCACTTAACGCCCATATGAATGCCAC
  CAACCACGCTATAGTTCAGACTCTGGTTCATCTGATGTTTCCTGACCACGTACCAAAGCCTTGTTGTGCT
  CCAACCAAATTAAATGCCATCTCTGTTCTGTACTTTGATGACAGCTCCAATGTCATTTTGAAAAAATATA
  GAAATATGGTAGTACGCTCATGTGGCTGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206456 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHLTVFLLKGIVGFLWSCWVLVGYAKGGLGDNHVHSSFIYRRLRNHERREIQREILSILGLPHRPRPFSP
  GKQASSAPLFMLDLYNAMTNEENPEESEYSVRASLAEETRGARKGYPASPNGYPRRIQLSRTTPLTTQSP
  PLASLHDTNFLNDADMVMSFVNLVERDKDFSHQRRHYKEFRFDLTQIPHGEAVTAAEFRIYKDRSNNRFE
  NETIKISIYQIIKEYTNRDADLFLLDTRKAQALDVGWLVFDITVTSNHWVINPQNNLGLQLCAETGDGRS
  INVKSAGLVGRQGPQSKQPFMVAFFKASEVLLRSVRAANKRKNQNRNKSSSHQDSSRMSSVGDYNTSEQK
  QACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCA
  PTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021073
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021073.4
• RefSeq Size: 2207 bp
• RefSeq ORF: 1365 bp
• Locus ID: 653
• UniProt ID: P22003
• Cytogenetics: 6p12.1
• Domains: TGFb_propeptide, TGF-beta
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
• Protein Pathways: Hedgehog signaling pathway, TGF-beta signaling pathway
• MW: 51.7 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Polymorphisms in this gene may be associated with osteoarthritis in human patients. This gene is differentially regulated in multiple human cancers. This gene encodes distinct protein isoforms that may be similarly proteolytically processed. [provided by RefSeq, Jul 2016]