Zfp219 (ZNF219) (NM_016423) Human Tagged ORF Clone

CAT#: RC206341

ZNF219 (Myc-DDK-tagged)-Human zinc finger protein 219 (ZNF219), transcript variant 1

Product Images

Circular map for RC206341

• 

  Western blot validation of overexpression lysate (Cat# LY420325) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223274 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp219
• Synonyms: ZFP219
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206341 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCTCACGTCCCCGCGCCCCGAGCGGCCACTTAGCGCCGTCGCCGCCGGCTTTCGACGGCGAGC
  TGGATCTGCAGCGATACTCCAACGGGCCAGCCGTGAGCGCAGGGTCGCTCGGGATGGGAGCGGTGAGCTG
  GTCTGAGAGTCGTGCAGGCGAACGGCGCTTCCCCTGCCCTGTATGCGGGAAGCGCTTCCGCTTCAACTCT
  ATCCTTGCTTTGCACCTGCGGGCGCACCCAGGAGCCCAGGCCTTCCAGTGCCCTCACTGCGGCCACCGCG
  CGGCGCAGCGGGCTCTGCTGCGCTCGCACCTGCGCACACACCAGCCCGAGCGCCCACGTAGTCCTGCTGC
  ACGCCTGTTGCTGGAGTTGGAAGAGCGCGCGCTACTACGCGAGGCCCGACTGGGGAGAGCCCGAAGCTCA
  GGGGGCATGCAGGCCACCCCTGCCACTGAGGGTCTGGCGCGGCCCCAGGCTCCTTCATCGTCCGCCTTCC
  GTTGCCCCTACTGCAAAGGCAAGTTTCGCACCTCGGCGGAGCGCGAACGCCACCTGCACATCCTGCATAG
  GCCCTGGAAGTGCGGCCTGTGCAGTTTCGGCTCCAGCCAGGAGGAGGAGCTGCTGCACCACAGCCTGACG
  GCCCACGGGGCTCCCGAGCGTCCCCTGGCGGCCACCTCCGCTGCGCCTCCGCCTCAGCCTCAGCCTCAGC
  CTCCACCCCAGCCCGAACCCAGATCAGTCCCCCAGCCGGAGCCGGAGCCGGAGCCCGAACGTGAGGCAAC
  CCCGACCACAGCTCCTGCCGCTCCCGAGGAGCCCCCAGCGCCTCCGGAGTTCCGCTGCCAAGTGTGCGGC
  CAGAGCTTTACACAGTCTTGGTTTCTCAAGGGCCACATGCGTAAGCACAAGGCCTCCTTCGATCATGCGT
  GTCCGGTGTGCGGCCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAGGTGCACGCCAGCAA
  GCTGGGCCCACTGCGTGCCCCGGGGCCTGCCTCCGGGCCTGCCCGCGCCCCCCAGCCTCCTGACCTCGGC
  CTGCTGGCCTATGAGCCGTTGGGCCCAGCGCTCCTCTTGGCCCCGGCTCCCACCCCGGCCGAGCGCCGTG
  AGCCCCCGAGCCTCTTGGGCTACCTGAGCCTGCGAGCTGGCGAGGGCCGGCCCAACGGCGAGGGTGCTGA
  GCCCGGTCCCGGCCGCAGCTTCGGAGGCTTCCGCCCGCTGTCCTCTGCTCTCCCGGCCCGGGCTCGCCGG
  CACCGTGCGGAGGAGCCTGAGGAAGAAGAGGAGGTGGTGGAGGCCGAGGAGGAAACCTGGGCCCGGGGCA
  GGTCGCTGGGCTCTCTGGCTTCCCTGCACCCGCGCCCGGGTGAGGGACCGGGGCACTCTGCCTCTGCTGC
  TGGGGCCCAGGCAAGATCGACCGCCACGCAGGAAGAGAATGGGCTGTTGGTTGGAGGGACCCGGCCTGAA
  GGGGGCCGGGGCGCCACCGGCAAGGATTGTCCTTTCTGCGGAAAATCTTTCCGCTCAGCACATCACCTCA
  AAGTGCATCTGCGAGTGCACACAGGCGAGCGGCCCTACAAGTGTCCGCACTGCGACTACGCGGGCACCCA
  GTCCGGCTCGCTCAAGTATCACCTACAGCGCCACCACCGGGAGCAGAGGAGCGGGGCCGGCCCCGGGCCA
  CCCCCGGAGCCACCGCCTCCTTCCCAGCGGGGTTCGGCCCCGCAATCTGGAGCCAAGCCGTCTCCGCAGC
  CTGCGACCTGGGTGGAGGGCGCCTCAAGTCCCCGGCCTCCTTCTAGCGGTGCTGGGCCGGGGTCCCGTCG
  GAAGCCCGCCAGCCCTGGGAGGACCCTGCGCAACGGGCGAGGCGGTGAGGCCGAACCCCTGGACCTGTCC
  TTGCGGGCAGGGCCGGGAGGCGAGGCCGGGCCTGGGGGTGCCCTCCACCGCTGCCTCTTCTGCCCGTTCG
  CCACTGGAGCCCCAGAGCTCATGGCCTTGCACCTTCAAGTGCACCACAGCCGCCGGGCTAGGGGCCGCCG
  GCCACCCCAGGCTGACGCGTCCCCGCCCTATGCCCGAGTACCATCAGGAGAGACCCCTCCCAGTCCTTCG
  CAGGAAGGGGAGGAGGGCTCCGGGCTGTCCAGACCCGGAGAGGCAGGGCTGGGGGGGCAAGAACGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206341 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGSRPRAPSGHLAPSPPAFDGELDLQRYSNGPAVSAGSLGMGAVSWSESRAGERRFPCPVCGKRFRFNS
  ILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPERPRSPAARLLLELEERALLREARLGRARSS
  GGMQATPATEGLARPQAPSSSAFRCPYCKGKFRTSAERERHLHILHRPWKCGLCSFGSSQEEELLHHSLT
  AHGAPERPLAATSAAPPPQPQPQPPPQPEPRSVPQPEPEPEPEREATPTTAPAAPEEPPAPPEFRCQVCG
  QSFTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMKVHASKLGPLRAPGPASGPARAPQPPDLG
  LLAYEPLGPALLLAPAPTPAERREPPSLLGYLSLRAGEGRPNGEGAEPGPGRSFGGFRPLSSALPARARR
  HRAEEPEEEEEVVEAEEETWARGRSLGSLASLHPRPGEGPGHSASAAGAQARSTATQEENGLLVGGTRPE
  GGRGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCDYAGTQSGSLKYHLQRHHREQRSGAGPGP
  PPEPPPPSQRGSAPQSGAKPSPQPATWVEGASSPRPPSSGAGPGSRRKPASPGRTLRNGRGGEAEPLDLS
  LRAGPGGEAGPGGALHRCLFCPFATGAPELMALHLQVHHSRRARGRRPPQADASPPYARVPSGETPPSPS
  QEGEEGSGLSRPGEAGLGGQER
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016423
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016423.3
• RefSeq Size: 3098 bp
• RefSeq ORF: 2169 bp
• Locus ID: 51222
• UniProt ID: Q9P2Y4
• Cytogenetics: 14q11.2
• Protein Families: Transcription Factors
• MW: 76.9 kDa
• Gene Summary: This gene is a member of the Kruppel-like zinc finger gene family. The encoded protein functions as a transcriptional repressor of the high mobility group nucleosome binding domain 1 protein, which is associated with transcriptionally active chromatin. [provided by RefSeq, Apr 2017]