SLC6A1 (NM_003042) Human Tagged ORF Clone

CAT#: RC206290

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SLC6A1 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1)



  "NM_003042" in other vectors (4)

Reconstitution Protocol

USD 558.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC6A1
Synonyms GABATHG; GABATR; GAT1; MAE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206290 representing NM_003042
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCAACGGCAGCAAGGTGGCCGACGGGCAGATCTCCACCGAGGTCAGCGAGGCCCCTGTGGCCA
ATGACAAGCCCAAAACCTTGGTGGTCAAGGTGCAGAAGAAGGCGGCAGACCTCCCCGACCGGGACACGTG
GAAGGGCCGCTTCGACTTCCTCATGTCCTGTGTGGGCTATGCCATCGGCCTGGGCAACGTCTGGAGGTTC
CCCTATCTCTGCGGGAAAAATGGTGGGGGAGCCTTCCTGATCCCCTATTTCCTGACACTCATCTTTGCGG
GGGTCCCACTCTTCCTGCTGGAGTGCTCCCTGGGCCAGTACACCTCCATCGGGGGGCTAGGGGTATGGAA
GCTGGCTCCTATGTTCAAGGGCGTGGGCCTTGCGGCTGCTGTGCTATCATTCTGGCTGAACATCTACTAC
ATCGTCATCATCTCCTGGGCCATTTACTACCTGTACAACTCCTTCACCACGACACTGCCGTGGAAACAGT
GCGACAACCCCTGGAACACAGACCGCTGCTTCTCCAACTACAGCATGGTCAACACTACCAACATGACCAG
CGCTGTGGTGGAGTTCTGGGAGCGCAACATGCATCAGATGACGGACGGGCTGGATAAGCCAGGTCAGATC
CGCTGGCCACTGGCCATCACGCTGGCCATCGCCTGGATCCTTGTGTATTTCTGTATCTGGAAGGGTGTTG
GCTGGACTGGAAAGGTGGTCTACTTTTCAGCCACATACCCCTACATCATGCTGATCATCCTGTTCTTCCG
TGGAGTGACGCTGCCCGGGGCCAAGGAGGGCATCCTCTTCTACATCACACCCAACTTCCGCAAGCTGTCT
GACTCCGAGGTGTGGCTGGATGCGGCAACCCAGATCTTCTTCTCATACGGGCTGGGCCTGGGGTCCCTGA
TCGCTCTCGGGAGCTACAACTCTTTCCACAACAATGTCTACAGGGACTCCATCATCGTCTGCTGCATCAA
TTCGTGCACCAGCATGTTCGCAGGATTCGTCATCTTCTCCATCGTGGGCTTCATGGCCCATGTCACGAAG
AGGTCCATTGCTGATGTGGCGGCCTCAGGCCCCGGGCTGGCGTTCCTGGCATACCCAGAGGCGGTGACCC
AGCTGCCTATCTCCCCACTCTGGGCCATCCTCTTCTTCTCCATGCTGTTGATGCTGGGCATTGACAGCCA
GTTCTGCACTGTGGAGGGCTTCATCACAGCCCTGGTGGATGAGTACCCCAGGCTCCTCCGCAACCGCAGA
GAGCTCTTCATTGCTGCTGTCTGCATCATCTCCTACCTGATCGGTCTCTCTAACATCACTCAGGGGGGTA
TTTATGTCTTCAAACTCTTTGACTACTACTCTGCCAGTGGCATGAGCCTGCTGTTCCTCGTGTTCTTTGA
ATGTGTCTCTATTTCCTGGTTTTACGGTGTCAACCGATTCTATGACAATATCCAAGAGATGGTTGGATCC
AGGCCCTGCATCTGGTGGAAACTCTGCTGGTCTTTCTTCACACCAATCATTGTGGCGGGCGTGTTCATTT
TCAGTGCTGTGCAGATGACGCAACTCACCATGGGAAACTATGTTTTCCCCAAGTGGGGCCAGGGTGTGGG
CTGGCTGATGGCTCTGTCTTCCATGGTCCTCATCCCCGGGTACATGGCCTACATGTTCCTCACCTTAAAG
GGCTCCCTGAAGCAGCGCATCCAAGTCATGGTCCAGCCCAGCGAAGACATCGTTCGCCCAGAGAATGGTC
CTGAGCAGCCCCAGGCGGGCAGCTCCACCAGCAAGGAGGCCTACATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206290 representing NM_003042
Red=Cloning site Green=Tags(s)

MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFDFLMSCVGYAIGLGNVWRF
PYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYY
IVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSMVNTTNMTSAVVEFWERNMHQMTDGLDKPGQI
RWPLAITLAIAWILVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLS
DSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTK
RSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRR
ELFIAAVCIISYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGS
RPCIWWKLCWSFFTPIIVAGVFIFSAVQMTQLTMGNYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLK
GSLKQRIQVMVQPSEDIVRPENGPEQPQAGSSTSKEAYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003042
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003042.2, NP_003033.2
RefSeq Size 4493 bp
RefSeq ORF 1800 bp
Locus ID 6529
UniProt ID P30531
Cytogenetics 3p25.3
Domains SNF
Protein Families Druggable Genome, Transmembrane
MW 66.9 kDa
Gene Summary The protein encoded by this gene is a gamma-aminobutyric acid (GABA) transporter that localizes to the plasma membrane. The encoded protein removes GABA from the synaptic cleft, restoring it to presynaptic terminals. [provided by RefSeq, Jan 2017]

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