MCM4 (NM_182746) Human Tagged ORF Clone

CAT#: RC206122

MCM4 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2

Product Images

Circular map for RC206122

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  Western blot validation of overexpression lysate (Cat# LY405345) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206122 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MCM4 protein (Cat# TP306122). The protein was produced from HEK293T cells transfected with MCM4 cDNA clone (Cat# RC206122) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCM4
• Synonyms: CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD; P1-CDC21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206122 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCCGCCCAGACGC
  CTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAGGATTCCACCTCCACGGGGGA
  GTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAGAGCCCTGCTGCGCAGGACGTGCTGTTTTCC
  AGCCCTCCCCAAATGCATTCTTCAGCTATCCCTCTTGACTTTGATGTTAGTTCACCACTGACATACGGCA
  CTCCCAGCTCTCGGGTAGAGGGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGA
  CCTGGGCTCTGCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTG
  GCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCAGCATGCAAAG
  AAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAGAAGAAGAAAATGTTGGCATAGA
  TATTACTGAACCTCTATACATGCAACGACTTGGGGAGATTAATGTTATTGGTGAGCCATTTTTAAATGTG
  AACTGTGAACACATCAAATCATTTGACAAAAATTTGTACAGACAACTCATCTCTTACCCACAGGAAGTTA
  TTCCAACTTTTGACATGGCTGTCAATGAAATCTTCTTTGACCGTTACCCTGACTCAATCTTAGAACATCA
  GATTCAAGTAAGACCATTCAACGCATTGAAGACTAAGAATATGAGAAACCTGAATCCAGAAGACATTGAC
  CAGCTCATCACCATCAGCGGCATGGTGATCAGGACATCCCAGCTGATTCCCGAGATGCAGGAGGCCTTCT
  TCCAGTGCCAAGTGTGTGCCCACACGACCCGGGTGGAGATGGACCGCGGCCGCATTGCAGAGCCCAGTGT
  GTGCGGGCGCTGCCACACCACCCACAGCATGGCACTCATCCACAACCGCTCCCTCTTCTCTGACAAGCAG
  ATGATCAAGCTTCAGGAGTCTCCGGAAGACATGCCTGCAGGGCAGACACCACACACAGTTATCCTGTTTG
  CTCACAATGATCTCGTTGACAAGGTCCAGCCTGGGGACAGAGTGAATGTTACAGGCATCTATCGAGCTGT
  GCCTATTCGAGTCAATCCAAGAGTGAGTAATGTGAAGTCTGTCTACAAAACCCACATTGATGTCATTCAT
  TATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGA
  AACGTGTGGAATTGCTTAAGGAACTTTCCAGGAAACCAGACATTTATGAGAGGCTTGCTTCAGCCTTGGC
  TCCAAGCATTTATGAACATGAAGATATAAAGAAGGGAATTTTGCTTCAGCTCTTTGGCGGGACAAGGAAG
  GATTTTAGTCACACTGGAAGGGGCAAATTTCGGGCTGAGATCAACATCTTGCTGTGTGGCGACCCTGGTA
  CCAGCAAGTCCCAGCTGCTGCAGTACGTGTACAACCTCGTCCCCAGGGGCCAGTACACGTCTGGGAAGGG
  CTCCAGTGCAGTTGGCCTCACTGCGTACGTAATGAAAGACCCTGAGACAAGGCAGCTGGTCCTGCAGACA
  GGTGCTCTTGTCCTGAGTGACAACGGCATCTGCTGTATCGATGAGTTCGACAAGATGAATGAAAGTACAA
  GATCGGTATTGCATGAAGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCT
  CAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCCATTGAGTCTCAGTGGAATCCTAAAAAAACAACC
  ATTGAAAACATCCAGCTGCCTCATACTTTATTATCAAGGTTTGATTTGATCTTCCTCATGCTGGACCCTC
  AGGACGAAGCCTATGACAGGCGTCTGGCTCACCACCTGGTCGCACTGTACTACCAGAGCGAGGAGCAGGC
  AGAGGAGGAGCTCCTGGACATGGCGGTGCTAAAGGACTACATTGCCTACGCGCACAGCACCATCATGCCG
  CGGCTAAGTGAGGAAGCCAGCCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATTGGCAGTAGCC
  GGGGAATGGTTTCTGCATACCCTCGACAGCTAGAGTCATTAATCCGCTTAGCAGAAGCCCATGCTAAAGT
  AAGATTGTCTAACAAAGTTGAAGCCATTGATGTGGAAGAGGCCAAACGCCTCCATCGGGAAGCTCTGAAG
  CAGTCTGCAACTGATCCCCGGACTGGCATCGTGGACATATCTATTCTTACTACGGGGATGAGTGCCACCT
  CTCGTAAACGGAAAGAAGAATTAGCTGAAGCATTGAAAAAGCTTATTTTATCTAAGGGCAAAACACCAGC
  TCTAAAATACCAGCAACTTTTTGAAGATATTCGGGGACAATCTGACATAGCAATTACTAAAGATATGTTT
  GAAGAAGCACTGCGTGCCCTGGCAGATGATGATTTCCTGACAGTGACTGGGAAGACCGTGCGCTTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206122 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFS
  SPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIV
  ASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNV
  NCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDID
  QLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQ
  MIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIH
  YRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRK
  DFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQT
  GALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTT
  IENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMP
  RLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALK
  QSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMF
  EEALRALADDDFLTVTGKTVRLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182746
• ORF Size: 2589 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182746.3
• RefSeq Size: 4800 bp
• RefSeq ORF: 2592 bp
• Locus ID: 4173
• UniProt ID: P33991
• Cytogenetics: 8q11.21
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• MW: 96.6 kDa
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]